Volume 6 Issue 3
May  2015
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2015  >  6(3): 162-168
Cao Jinglin, Wang Miao, Wang Yang, et al. Protective effect of creatine phosphate on isolated rat liver against cold preservation[J]. ORGAN TRANSPLANTATION, 2015, 6(3): 162-168. doi: 10.3969/j.issn.1674-7445.2015.03.007
Citation: Cao Jinglin, Wang Miao, Wang Yang, et al. Protective effect of creatine phosphate on isolated rat liver against cold preservation[J]. ORGAN TRANSPLANTATION, 2015, 6(3): 162-168. doi: 10.3969/j.issn.1674-7445.2015.03.007
shu

Protective effect of creatine phosphate on isolated rat liver against cold preservation

doi: 10.3969/j.issn.1674-7445.2015.03.007

  • Department of Hepatobiliary Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, China

More Information
  • Corresponding author: Dou Jian, Email:2423978344@qq.com
  • Received Date: 2015-01-26
    Available Online: 2021-01-19
  • Publish Date: 2015-05-15
    Abstract
  •   Objective  To discuss the protective effect of creatine phosphate(CP) on isolated rat liver against cold preservation.  Methods  Isolated perfused rat liver model under simple cold preservation was established. The liver of the control group was perfused with pure University of Wisconsin(UW) solution. With UW solution as the base fluid, the liver of the low-dose group was perfused with 1 g/100 ml CP in UW solution; the liver of the middle-dose group was perfused with 2 g/100 ml CP in UW solution; the liver of the high-dose group was perfused with 3 g/100 ml CP in UW solution. The livers of each group were cold preserved in the corresponding perfusion fluid at 4℃. The content of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) in preservation solution in infrahepatic vena cava were determined. The content of malondialdehyde (MDA) and activity of myeloperoxidase (MPO) in liver tissues were detected. The apoptosis index (AI) of liver cells in liver tissues and positive expression rate of NF-κB in liver tissues were observed. Pathologic changes of liver tissues were observed under optical microscope.  Results  At 12 h after the cold preservation, the content of ALT and LDH in the rat livers of low-, middle-and high-dose groups were lower than those of the control group (all in P < 0.05). At 18 h after the cold preservation, the content of MDA and MPO in the liver tissues of low-, middle-and high-dose groups were lower than those of the control group (all in P < 0.05). At 12 h and 18 h after the cold preservation, AI and positive expression rate of NF-κB in liver cells in the rat livers of low-, middle-and high-dose groups were lower than those of the control group (all in P < 0.05). At 24 h after the cold preservation, the content of ALT and MDA in preservation solution of the high-dose group was obviously higher than that of the control group as well as the low-and middle-dose groups (all in P < 0.05). The results of pathological examination indicated that the injuries to the livers of the high-, middle-and low-dose groups were obviously lighter than that of the control group. There was no obvious difference among each dose group.  Conclusions  CP in UW solution may well protect the isolated rat liver against cold preservation, which is better than pure UW solution.

     

    • FullText(HTML)
  • loading
    • References(22)
  • [1]
    万钧, 范上达.肝癌肝移植的临床及基础研究[J].临床肝胆病杂志, 2012, 28(4):243-244. http://www.cnki.com.cn/Article/CJFDTOTAL-LCGD201204003.htm

    Wang J, Fan SD. Clinical and basic research on liver transplantation for hepatocellular carcinoma[J]. J Clin Hepatol, 2012, 28(4):243-244. http://www.cnki.com.cn/Article/CJFDTOTAL-LCGD201204003.htm
    [2]
    侯萍, 李剑平.器官移植前保存的理论研究与临床应用[J].中国组织工程研究, 2012, 16(5):895-898. http://www.cnki.com.cn/Article/CJFDTOTAL-XDKF201205040.htm

    Hou P, Li JP. Clinical application and theoretical study of organ preservation before transplantation[J]. Chin J Tissue Eng Res, 2012, 16(5):895-898. http://www.cnki.com.cn/Article/CJFDTOTAL-XDKF201205040.htm
    [3]
    钱伟民, 张中明, 张伟, 等.磷酸肌酸、左旋精氨酸对移植供心保护作用的实验研究[J].徐州医学院学报, 2006, 26(2):112-115. http://www.cnki.com.cn/Article/CJFDTOTAL-XZYX200602007.htm

    Qian WM, Zhang ZM, Zhang W, et al. Experimental study on the protective effects of creatine phosphate and L-arginine on donor heart in rats[J]. Acta Acad Med Xuzhou, 2006, 26(2):112-115. http://www.cnki.com.cn/Article/CJFDTOTAL-XZYX200602007.htm
    [4]
    王宇, 周杰, 蒋晓青.丹参对原位肝移植大鼠供肝冷保存再灌注损伤的保护作用[J].中国组织工程研究与临床康复, 2008, 12(5):801-804. http://www.cnki.com.cn/Article/CJFDTOTAL-XDKF200805005.htm

    Wang Y, Zhou J, Jiang XQ. Effects of radix salvia miltiorrhiz on protecting donor liver against cold preservation and reperfusion injury during orthotopic liver transplantation in rats[J]. Chin J Clin Rehabil Tissue Eng Res, 2008, 12(5):801-804. http://www.cnki.com.cn/Article/CJFDTOTAL-XDKF200805005.htm
    [5]
    Gökakın AK, Atabey M, Deveci K, et al. The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn:a biochemical and histopathological study[J]. Ulus Travma Acil Cerrahi Derg, 2014, 20(5):319-327. doi: 10.5505/tjtes.2014.39586
    [6]
    Ramalho FS, Fernandez-Monteiro I, Rosello-Catafau J, et al. Hepatic microcirculatory failure[J]. Acta Cir Bras, 2006, 21(Suppl 1):48-53.
    [7]
    Franco-Gou R, Mosbah IB, Serafin A, et al. New preservation strategies for preventing liver grafts against cold ischemia reperfusion injury[J]. J Gastroenterol Hepatol, 2007, 22(7):1120-1126. doi: 10.1111/jgh.2007.22.issue-7
    [8]
    Puhl G, Schaser KD, Pust D, et al. Initial hepatic microcirculation correlates with early graft function in human orthotopic liver transplantation[J]. Liver Transpl, 2005, 11(5):555-563. doi: 10.1002/lt.v11:5
    [9]
    Nieuwenhuijs VB, De Bruijn MT, Padbury RT, et al. Hepatic ischemia-reperfusion injury:roles of Ca2+ and other intracellular mediators of impaired bile flow and hepatocyte damage[J]. Dig Dis Sci, 2006, 51(6):1087-1102. doi: 10.1007/s10620-006-8014-y
    [10]
    Riddle-Taylor E, Nagasaki K, Lopez J, et al. Mutations to bid cleavage sites protect hepatocytes from apoptosis after ischemia/reperfusion injury[J]. Transplantation, 2007, 84(6):778-785. doi: 10.1097/01.tp.0000281555.18782.2b
    [11]
    McAnulty JF, Reid TW, Waller KR, et al. Successful six-day kidney preservation using trophic factor supplemented media and simple cold storage[J].Am J Transplant, 2002, 2(8):712-718. doi: 10.1034/j.1600-6143.2002.20805.x
    [12]
    Desurmont T, Giraud S, Cau J, et al. Trophic factor and FR167653 supplementation during cold storage rescue chronic renal injury[J].J Urol, 2011, 185(3):1139-1146. doi: 10.1016/j.juro.2010.10.032
    [13]
    Ambiru S, Uryuhara K, Talpe S, et al. Improved survival of orthotopic liver allograft in swine by addition of trophic factors to University of Wisconsin solution[J].Transplantation, 2004, 77(2):302-319. doi: 10.1097/01.TP.0000100468.94126.AF
    [14]
    Van Beek JH. Adenine nucleotide-creatine-phosphate module in myocardial metabolic system explains fast phase of dynamic regulation of oxidative phosphorylation[J]. Am J Physiol Cell Physiol, 2007, 293(3):815-829. doi: 10.1152/ajpcell.00355.2006
    [15]
    Inserte J, Barba I, Hernando V, et al. Effect of acidic reperfusion on prolongation of intracellular acidosis and myocardial salvage[J]. Cardiovasc Res, 2008, 77(4):782-790. https://www.researchgate.net/publication/5790980_Effect_of_acidic_reperfusion_on_prolongation_of_intracellular_acidosis_and_myocardial_salvage._Cardiovasc_Res
    [16]
    Masuda Y, Vaziri ND, Takasu C, et al. Salutary effect of pre-treatment with an Nrf2 inducer on ischemia reperfusion injury in the rat liver[J]. Gastroenterol Hepatol, 2014, 1(1):1-7. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280567/
    [17]
    Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, et al. Inflammation, oxidative stress, and obesity[J].Int J Mol Sci, 2011, 12(5):3117-3132. http://cn.bing.com/academic/profile?id=1583401207&encoded=0&v=paper_preview&mkt=zh-cn
    [18]
    葛缅, 池信锦, 刘德昭.大鼠自体原位肝移植模型中肠道羟自由基、丙二醛和总抗氧化能力的变化[J].中国病理生理杂志, 2012, 28(4):723-726. http://www.cnki.com.cn/Article/CJFDTOTAL-ZBLS201204030.htm

    Ge M, Chi XJ, Liu DZ, et al. Changes of hydroxyl radical, malondialdehyde and total antioxidant capacity in intestines after orthotopic liver autotransplantation in rats[J]. Chin J Pathophysiol, 2012, 28(4):723-726. http://www.cnki.com.cn/Article/CJFDTOTAL-ZBLS201204030.htm
    [19]
    Locatelli F, Gauly A, Czekalski S, et al. The MPO Study:just a European HEMO Study or something very different?[J]. Blood Purif, 2008, 26(1):100-104. doi: 10.1159/000110574
    [20]
    王淼, 曹经琳, 窦剑.磷酸肌酸对心肌缺血-再灌注损伤的保护机制及其在肝移植中的拓展应用[J].器官移植, 2013, 4(4):243-246. http://www.organtranspl.com/browse/detail/cat/37/id/358

    Wang M, Cao JL, Dou J, et al. Protection mechanism of creatine phosphate in myocardium ischemia reperfusion injury and its further application in liver transplantation[J]. Organ Transplant, 2013, 4(4):243-246. http://www.organtranspl.com/browse/detail/cat/37/id/358
    [21]
    Lavorgna A, Harhaj EW. Regulation of HTLV-1 tax stability, cellular trafficking and NF-κB activation by the ubiquitin-proteasome pathway[J]. Viruses, 2014, 6(10):3925-3943. doi: 10.3390/v6103925
    [22]
    Zhao Y, Feng G, Wang Y, et al. A key mediator, PTX3, of IKK/IκB/NF-κB exacerbates human umbilical vein endothelial cell injury and dysfunction[J].Int J Clin Exp Pathol, 2014, 7(11):7699-7707. http://www.ijcep.com/files/ijcep0002338.pdf
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (107) PDF downloads(6) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return