Volume 6 Issue 3
May  2015
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2015  >  6(3): 157-161, 168
Zhou Lina, Cui Xiaojun, Su Kaixin, et al. Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve[J]. ORGAN TRANSPLANTATION, 2015, 6(3): 157-161, 168. doi: 10.3969/j.issn.1674-7445.2015.03.006
Citation: Zhou Lina, Cui Xiaojun, Su Kaixin, et al. Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve[J]. ORGAN TRANSPLANTATION, 2015, 6(3): 157-161, 168. doi: 10.3969/j.issn.1674-7445.2015.03.006
shu

Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve

doi: 10.3969/j.issn.1674-7445.2015.03.006

  • Department of Anatomy, Guangdong Medical College, Zhanjiang 524023, China

More Information
  • Corresponding author: Zhou Lina, Email:21456783@qq.com
  • Received Date: 2015-01-25
    Available Online: 2021-01-19
  • Publish Date: 2015-05-15
    Abstract
  •   Objective  To discuss the effect of bone marrow mesenchymal stem cell (BMSC) as the seed cell transplantation of tissue-engineered artificial nerve in the treatment of peripheral nerve injury.  Methods  BMSC was obtained from the bone marrow of adult rat through isolation and culture and combined with acellular nerve scaffold to construct'tissue-engineered artificial nerve'. After transplantation, rats were divided into two groups, the BMSC+acellular nerve conduit group(BMSC treatment group)and the empty cell conduit group(negative control group)with 5 rats in each group. Sciatic functional index (SFI) of the affected side of rats was compared between two groups at 2 weeks, 4 weeks and 8 weeks after the surgery. Moreover, the sciatic conduction, recovery rate of tricipital muscle wet weight and other repair effects of the affected side were compared between two groups at 8 weeks after the surgery.  Results  The indicators of BMSC treatment group, including SFI assessed at 2 weeks, 4 weeks and 8 weeks after the surgery as well as the sciatic conduction and recovery rate of tricipital muscle wet weight assessed at 8 weeks after the surgery, were better than those of the negative control group(all in P < 0.05).  Conclusions  BMSC combined with tissue-engineered artificial nerve of acellular nerve scaffold can effectively promote nerve regeneration and function recovery.

     

    • FullText(HTML)
  • loading
    • References(16)
  • [1]
    Erakat MS, Chuang SK, Shanti RM, et al. Interval between injury and lingual nerve repair as a prognostic factor for success using type Ⅰ collagen conduit[J]. J Oral Maxillofac Surg, 2013, 71(5):833-838. doi: 10.1016/j.joms.2011.11.026
    [2]
    Bonilla C, Zurita M, Otero L, et al. The severity of brain damage determines bone marrow stromal cell therapy efficacy in a traumatic brain injury model[J]. J Trauma Acute Care Surg, 2012, 72(5):1203-1212. doi: 10.1097/TA.0b013e318248bdcf
    [3]
    Zhou LN, Zhang JW, Wang JC, et al. Bone marrow stromal and Schwann cells from adult rats can interact synergistically to aid in peripheral nerve repair even without intercellular contact in vitro[J]. J Tissue Eng Regen Med, 2012, 6(7):579-588. doi: 10.1002/term.v6.7
    [4]
    Elias PZ, Spector M. Implantation of a collagen scaffold seeded with adult rat hippocampal progenitors in a rat model of penetrating brain injury[J]. J Neurosci Methods, 2012, 209(1):199-211. doi: 10.1016/j.jneumeth.2012.06.003
    [5]
    Yu H, Cao B, Feng M, et al. Combinated transplantation of neural stem cells and collagen type Ⅰ promote functional recovery after cerebral ischemia in rats[J]. Anat Rec, 2010, 293(5):911-917. doi: 10.1002/ar.20941
    [6]
    Wang D, Huang X, Fu G, et al. A simple model of radial nerve injury in the rhesus monkey to evaluate peripheral nerve repair[J]. Neural Regen Res, 2014, 9(10):1041-1046. doi: 10.4103/1673-5374.133166
    [7]
    Mimura T, Dezawa M, Kanno H, et al. Peripheral nerve regeneration by transplantation of bone marrow stromal cell-derived Schwann cells in adult rats[J]. J Neurosurg, 2004, 101(5):806-812. doi: 10.3171/jns.2004.101.5.0806
    [8]
    Wang D, Liu XL, Zhu JK, et al. Repairing large radial nerve defects by acellular nerve allografts seeded with autologous bone marrow stromal cells in a monkey model[J]. J Neurotrauma, 2010, 27(10):1935-1943. doi: 10.1089/neu.2010.1352
    [9]
    Mohammadi R, Azizi S, Delirezh N, et al. The use of undifferentiated bone marrow stromal cells for sciatic nerve regeneration in rats[J]. Int J Oral Maxillofac Surg, 2012, 41(5):650-656. doi: 10.1016/j.ijom.2011.10.028
    [10]
    Lin W, Chen X, Wang X, et al. Adult rat bone marrow stromal cells differentiate into Schwann cell-like cells in vitro[J]. In Vitro Cell Dev Biol Anim, 2008, 44(1/2):31-40. doi: 10.1007/s11626-007-9064-y
    [11]
    柴树宏, 鲍艳春.人脐带间充质干细胞与骨髓间充质干细胞分化为胰岛素分泌细胞的能力比较[J].实用医学杂志, 2014, 30(1):52-54. http://www.cnki.com.cn/Article/CJFDTOTAL-SYYZ201401021.htm

    Chai SH, Bao YC. Comparison of the ability of umbilical cord mesenchymal stem cells and bone marrow mesenchymal stem cells to differentiate into insulin-producing cells[J]. J Pract Med, 2014, 30(1):52-54. http://www.cnki.com.cn/Article/CJFDTOTAL-SYYZ201401021.htm
    [12]
    Johnson EO, Zoubos AB, Soucacos PN. Regeneration and repair of peripheral nerves[J]. Injury, 2005, 36(Suppl 4):S24-S29. http://www.ncbi.nlm.nih.gov/pubmed/16288757
    [13]
    Hou SY, Zhang HY, Quan DP, et al. Tissue-engineered peripheral nerve grafting by differentiated bone marrow stromal cells[J]. Neuroscience, 2006, 140(1):101-110. doi: 10.1016/j.neuroscience.2006.01.066
    [14]
    尹东亮, 孙翀, 朱焕斌, 等.CTLA4-Ig基因修饰骨髓间充质干细胞抑制大鼠肝移植排斥反应[J].器官移植, 2014, 5(4):231-236. http://www.organtranspl.com/browse/detail/cat/33/id/210

    Yin DL, Sun C, Zhu HB, et al. Bone marrow mesenchymal stem cell modified by CTLA4-Ig gene can inhibit the rejection of liver transplantation in rats[J]. Organ Transplant, 2014, 5(4):231-236. http://www.organtranspl.com/browse/detail/cat/33/id/210
    [15]
    王月, 牛坚.同种大鼠骨髓间充质干细胞诱导调节性B淋巴细胞的免疫负调节作用[J].中华器官移植杂志, 2013, 34(7):428-431. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201307012

    Wang Y, Niu J. An experimental study on the role of MSCs in inducing regulatory B cells and the underlying mechanisms[J]. Chin J Organ Transplant, 2013, 34(7):428-431. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201307012
    [16]
    陈规划, 杨扬, 张琪, 等.间充质干细胞应用现状及展望[J/CD].中华肝脏外科手术学电子杂志, 2013, 2(4):1-3. http://www.cnki.com.cn/article/cjfdtotal-zhzw201304001.htm

    Chen GH, Yang Y, Zhang Q, et al. Application status and prospects of mesenchymal stem cells[J/CD]. Chin J Hepatic Surg:Electr Edit, 2013, 2(4):1-3. http://www.cnki.com.cn/article/cjfdtotal-zhzw201304001.htm
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (99) PDF downloads(4) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return