低温机械灌注对大鼠肾脏炎症因子表达水平的影响

罗德; 刘江; 周鹏程; 王飘; 李栩嘉; 林号民; 苏松

doi:10.3969/j.issn.1674-7445.2023132

低温机械灌注对大鼠肾脏炎症因子表达水平的影响

doi: 10.3969/j.issn.1674-7445.2023132

罗德^1,,
刘江²,
周鹏程³,
王飘¹,
李栩嘉¹,
林号民¹,
苏松^1, ,

1.
646000　四川泸州，西南医科大学附属医院肝胆外科
2.
肾病内科
3.
西南医科大学附属中医医院肾病科

基金项目: 四川省科技厅联合创新项目（2022YFS0632-B4）

详细信息

作者简介:

通讯作者:
苏松（ORCID：0000-0002-0894-1361），博士，研究方向为器官移植与免疫，Email：13882778554@163.com

中图分类号: R617， R364.5
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出版历程
- 收稿日期: 2023-07-05
- 录用日期: 2023-09-11
- 网络出版日期: 2023-09-19
- 刊出日期: 2023-11-09

Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

1.
Department of Hepatobiliary Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China

More Information

Corresponding author: Su Song, Email: 13882778554@163.com

摘要

摘要: 目的探讨低温机械灌注（HMP）对大鼠肾脏炎症因子表达水平的影响。方法雄性大鼠30只，随机分为对照组（Control组），静态冷保存组（SCS组）和HMP组，每组10只。记录HMP过程中流速、肾内阻力和灌注流出液pH值。收集每组肾脏组织，采用逆转录聚合酶链反应（RT-PCR）检测CXC趋化因子配体（CXCL）1、CXCL2、干扰素（IFN）-β1、IFN-α4、CC趋化因子配体（CCL）2、CCL20、白细胞介素（IL）-17α、IL-17C和肿瘤坏死因子（TNF）-α的信使RNA（mRNA）表达水平，苏木素-伊红（HE）染色观察肾脏组织病理学改变。结果 HMP过程中的流速、肾内阻力保持稳定，灌注流出液pH值缓慢降低。RT-PCR结果显示，与Control组比较，SCS组和HMP组CXCL1、CXCL2、CCL2、CCL20、IL-17α、IL-17C和TNF-α的mRNA相对表达量升高；与SCS组比较，HMP组CXCL1、CXCL2、CCL2、CCL20、IL-17α和TNF-α的mRNA相对表达量升高（均为P<0.05）。HE染色结果显示，Control组肾脏细胞形态正常，SCS组出现明显的上皮坏死、胞质空泡化、刷状缘丢失、上皮脱落，与SCS组比较，HMP组病理改变程度减轻。结论 HMP能激活肾脏炎症反应，抑制HMP过程中的炎症反应激活有望进一步提高移植物保存效果。
- 肾移植 /
- 低温机械灌注 /
- 静态冷保存 /
- 炎症因子 /
- 白细胞介素 /
- 干扰素 /
- 肿瘤坏死因子 /
- 趋化因子
Abstract: Objective To evaluate the effect of hypothermic machine perfusion (HMP) on the expression levels of inflammatory cytokines in rat kidney. Methods Thirty male rats were randomly divided into the control (Control group), static cold storage group (SCS group) and HMP group, with 10 rats in each group. The velocity, intrarenal resistance and pH value of perfusion effluent were recorded during HMP. The expression levels of CXC chemokine ligand (CXCL)1, CXCL2, interferon (IFN)-β1, IFN-α4, CC chemokine ligand (CCL)2, CCL20, interleukin (IL)-17α, IL-17C and tumor necrosis factor (TNF)-α messenger RNA (mRNA) in renal tissues were evaluated by reverse transcription polymerase chain reaction (RT-PCR). Pathological changes of the kidney were observed by hematoxylin-eosin (HE) staining. Results During HMP, the velocity and intrarenal resistance remained stable, and the pH value of perfusion effluent was decreased slowly. RT-PCR showed that the relative expression levels of CXCL1, CXCL2, CCL2, CCL20, IL-17α, IL-17C and TNF-α mRNA in the SCS and HMP groups were higher compared with those in the Control group. Compared with the SCS group, the relative expression levels of CXCL1, CXCL2, CCL2, CCL20, IL-17α and TNF-α mRNA were up-regulated in the HMP group (all P<0.05). HE staining revealed that the morphology of renal cells was normal in the Control group, whereas evident epithelial necrosis, cytoplasmic vacuolation, brush border loss and epithelial shedding were observed in the SCS group. Compared with the SCS group, pathological changes in the HMP group were alleviated. Conclusions HMP may activate renal inflammation, and inhibiting the activation of inflammation during HMP is expected to further improve the effect of allograft preservation.
- Kidney transplantation /
- Hypothermic mechanical perfusion /
- Static cold storage /
- Inflammatory factor /
- Interleukin /
- Interferon /
- Tumor necrosis factor /
- Chemokine

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图 1 HMP过程中各参数变化

注：A图为HMP过程中流速的变化；B图为HMP过程中肾内阻力的变化；C图为HMP过程中静脉流出液pH值的变化。

Figure 1. Changes of parameters during HMP

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图 2 各组肾脏炎症因子mRNA表达水平

注：与Control组比较，^aP< 0.05；与SCS组比较，^bP< 0.05。

Figure 2. mRNA expression levels of inflammatory factors of kidneys in each group

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图 3 各组肾脏组织病理学改变（HE，×400）

注：A图为Control组；B图为SCS组；C图为HMP组。绿色箭头为上皮坏死，黑色箭头为刷状缘丢失，蓝色箭头为胞质空泡化，红色箭头为上皮脱落。

Figure 3. Histopathological changes of the kidney tissues in each group

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