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人牙龈间充质干细胞对B细胞的作用及机制研究

张楷 陈柯妍 李凯 马铁梁 古鉴

张楷, 陈柯妍, 李凯, 等. 人牙龈间充质干细胞对B细胞的作用及机制研究[J]. 器官移植, 2020, 11(2): 253-258. doi: 10.3969/j.issn.1674-7445.2020.02.011
引用本文: 张楷, 陈柯妍, 李凯, 等. 人牙龈间充质干细胞对B细胞的作用及机制研究[J]. 器官移植, 2020, 11(2): 253-258. doi: 10.3969/j.issn.1674-7445.2020.02.011
Zhang Kai, Chen Keyan, Li Kai, et al. Effect and mechanism of human gingival mesenchymal stem cell on B cells[J]. ORGAN TRANSPLANTATION, 2020, 11(2): 253-258. doi: 10.3969/j.issn.1674-7445.2020.02.011
Citation: Zhang Kai, Chen Keyan, Li Kai, et al. Effect and mechanism of human gingival mesenchymal stem cell on B cells[J]. ORGAN TRANSPLANTATION, 2020, 11(2): 253-258. doi: 10.3969/j.issn.1674-7445.2020.02.011

人牙龈间充质干细胞对B细胞的作用及机制研究

doi: 10.3969/j.issn.1674-7445.2020.02.011
基金项目: 

江苏省2013年度“六大人才高峰”高层次人才资助项目 2013-WSN-025

宜兴市科技创新专项资金项目 2017SF06

详细信息
    通讯作者:

    张楷,男,1974年生,博士,主任医师,研究方向为肝移植,Email:zk_104@163.com

  • 中图分类号: R617, R392.4

Effect and mechanism of human gingival mesenchymal stem cell on B cells

More Information
  • 摘要:   目的  探讨人牙龈间充质干细胞(GMSC)对B细胞增殖与分化的调节功能及其相关分子机制。  方法  分离提取GMSC,从外周血分选出B细胞。将GMSC或纤维母细胞分别与B细胞进行体外共培养,设为GMSC组和纤维母细胞组,检测两组B细胞的扩增情况;检测两组细胞上清中IgG1与IgM的表达情况;检测两组细胞白细胞介素(IL)-6、Perforin、干扰素(IFN)-γ和肿瘤坏死因子(TNF)-α的分泌情况;检测两组B细胞表达IL-10与转化生长因子(TGF)-β的水平;检测两组B细胞中PC-1的表达情况;检测GMSC调控B细胞功能的信号通路;检测GMSC对B细胞活化T细胞功能的调控。  结果  与纤维母细胞组比较,GMSC组B细胞的扩增明显减弱(P < 0.05),GMSC与B细胞共培养后,能明显抑制B细胞分泌IgG1和IgM,明显抑制IL-6、Perforin、IFN-γ和TNF-α的分泌(均为P < 0.05)。与纤维母细胞组比较,GMSC组中的IL-10与TGF-β分泌量更高(均为P < 0.05)。GMSC组中PC-1的表达水平明显降低(P < 0.05)。加入TGF-β受体抑制剂ALK5后,GMSC对B细胞的抑制作用明显减弱(P < 0.05)。与纤维母细胞组比较,GMSC组的B细胞活化和扩增T细胞的能力明显减弱(P < 0.05)。  结论  GMSC对B细胞及其介导的免疫反应具有抑制功能,可通过TGF-β信号通路抑制B细胞的活化以及其他相关功能。

     

  • 图  1  GMSC细胞标志物表型的表达情况

    Figure  1.  Expression of GMSC cell marker phenotypes

    图  2  GMSC对B细胞扩增的调节情况

    注:与纤维母细胞组比较,aP < 0.05。

    Figure  2.  Regulation of GMSC in B cell proliferation

    图  3  GMSC对B细胞分泌功能的调节情况

    注:A图示B细胞分泌IgG1的情况;B图示B细胞分泌IgM的情况;C图示B细胞分泌细胞因子的情况;与纤维母细胞组比较,aP < 0.05。

    Figure  3.  Regulation of GMSC in B cell secretion function

    图  4  GMSC对B细胞分化的调节情况

    注:A图示B细胞表达IL-10与TGF-β的水平,与纤维母细胞组比较,aP < 0.05;B图示B细胞中PC-1的表达情况。

    Figure  4.  Regulation of GMSC in B cell differentiation

    图  5  ALK5在GMSC调节B细胞中的作用

    Figure  5.  The effect of ALK5 on the regulation of B cell by GMSC

    图  6  GMSC对B细胞活化T细胞能力的影响

    Figure  6.  Effect of GMSC on the ability of B cell toactivate T cell

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出版历程
  • 收稿日期:  2020-01-14
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2020-03-15

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