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氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响

程骁 杨家杰 钟金淑子

程骁, 杨家杰, 钟金淑子. 氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响[J]. 器官移植, 2018, 9(2): 116-121. doi: 10.3969/j.issn.1674-7445.2018.02.005
引用本文: 程骁, 杨家杰, 钟金淑子. 氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响[J]. 器官移植, 2018, 9(2): 116-121. doi: 10.3969/j.issn.1674-7445.2018.02.005
Cheng Xiao, Yang Jiajie, Sookjia Kim Chung. Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/ progenitor cells[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 116-121. doi: 10.3969/j.issn.1674-7445.2018.02.005
Citation: Cheng Xiao, Yang Jiajie, Sookjia Kim Chung. Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/ progenitor cells[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 116-121. doi: 10.3969/j.issn.1674-7445.2018.02.005

氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响

doi: 10.3969/j.issn.1674-7445.2018.02.005
基金项目: 

国家自然科学基金 81303115

国家自然科学基金 81774042

国家自然科学基金 81771353

广东省自然科学基金 S2013040016915

广州市科技计划 201604020003

中国博士后项目 BBK42913K09

中国博士后项目 201003345

中国博士后项目 BBH429151701

详细信息
    作者简介:

    程骁,女,1983年生,博士,副教授,研究方向为中西医结合脑血管病临床及基础,Email: chengxiaolucky@126.com

    通讯作者:

    钟金淑子,女,1956年生,博士,教授,研究方向为干细胞与脑血管病,Email: 28868599@qq.com

  • 中图分类号: R742

Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/ progenitor cells

More Information
  • 摘要:   目的  探讨氧糖剥夺-再灌注(OGD-R)星型胶质细胞内皮素(ET)-1过表达对神经干/祖细胞(NSPCs)增殖的影响。  方法  构建阴性对照星型胶质细胞(C6-Mock)和ET-1过表达的星形胶质细胞(C6-ET-1)OGD-R模型,并构建星型胶质细胞与NSPCs Transwell共培养体系。对星型胶质细胞及原代NSPCs进行形态观察及鉴定;将细胞分为以下4组进行共培养:C6-Mock+NSPCs组,OGD-R+C6-Mock+NSPCs组,C6-ET-1+NSPCs组,OGD-R+C6-ET-1+NSPCs组,共培养0、24、48、72 h,分析各组NSPCs神经球直径。  结果  C6-Mock及C6-ET-1均呈现Ⅰ型星型胶质细胞的纤维状形态,神经胶质酸性纤维蛋白(GFAP)表达于这两种细胞的胞质中。原代NSPCs的巢蛋白(nestin)染色阳性。共培养后的48 h及72 h,OGD-R+C6-Mock+NSPCs组神经球的直径明显大于C6-Mock+NSPCs组的直径,OGD-R+C6-ET-1+NSPCs组神经球的直径明显大于C6-ET-1+NSPCs组的直径,同时OGD-R+C6-ET-1+NSPCs组神经球的直径明显大于OGD-R+C6-Mock+NSPCs组的直径,差异均有统计学意义(均为P < 0.05)。  结论  OGD-R星型胶质细胞促进NSPCs的增殖,且ET-1过表达进一步促进了NSPCs的增殖。

     

  • 图  1  相差显微镜下C6-Mock和C6-ET-1细胞的形态(×200)

    Figure  1.  Morphology of C6-Mock and C6-ET-1 under phase contrast microscope

    图  2  荧光显微镜下C6-Mock和C6-ET-1的GFAP表达(免疫荧光,×400)

    Figure  2.  GFAP expression of C6-Mock and C6-ET-1 under fluorescence microscope

    图  3  相差显微镜和荧光显微镜下原代培养神经球的形态及nestin表达

    A图为相差显微镜下的NSPCs神经球;B图为荧光显微镜下的NSPCs神经球

    Figure  3.  Nestin expression and morphology of primary culture neurosphere under phase contrast microscope and fluorescence microscope

    图  4  各组细胞共培养不同时间神经球直径的比较

    与C6-Mock+NSPCs组比较,aP<0.05;与OGD-R+C6-Mock+NSPCs组比较,bP<0.05;与C6-ET-1+NSPCs组比较,cP<0.05

    Figure  4.  Comparison of the diameter of neurospheres among each group of cells at different time after co-culture

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出版历程
  • 收稿日期:  2018-02-15
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2018-03-15

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