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人脐带间充质干细胞对小鼠肝缺血-再灌注损伤后肝内CD4+T细胞的影响

周朝荣 吕海金 孙瑶 安玉玲 范明明 易慧敏

周朝荣, 吕海金, 孙瑶, 等. 人脐带间充质干细胞对小鼠肝缺血-再灌注损伤后肝内CD4+T细胞的影响[J]. 器官移植, 2018, 9(2): 103-109. doi: 10.3969/j.issn.1674-7445.2018.02.003
引用本文: 周朝荣, 吕海金, 孙瑶, 等. 人脐带间充质干细胞对小鼠肝缺血-再灌注损伤后肝内CD4+T细胞的影响[J]. 器官移植, 2018, 9(2): 103-109. doi: 10.3969/j.issn.1674-7445.2018.02.003
Zhou Chaorong, Lyu Haijin, Sun Yao, et al. Effect of human umbilical cord mesenchymal stem cells on CD4+ T cells in liver after hepatic ischemia-reperfusion injury in mice[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 103-109. doi: 10.3969/j.issn.1674-7445.2018.02.003
Citation: Zhou Chaorong, Lyu Haijin, Sun Yao, et al. Effect of human umbilical cord mesenchymal stem cells on CD4+ T cells in liver after hepatic ischemia-reperfusion injury in mice[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 103-109. doi: 10.3969/j.issn.1674-7445.2018.02.003

人脐带间充质干细胞对小鼠肝缺血-再灌注损伤后肝内CD4+T细胞的影响

doi: 10.3969/j.issn.1674-7445.2018.02.003
基金项目: 

广东省科技计划项目 2014A020211010

详细信息
    作者简介:

    周朝荣,男,1989年生,硕士研究生,研究方向为干细胞治疗,Email: zhouchr3@hotmail.com

    通讯作者:

    易慧敏,女,1975年生,博士,主任医师,研究方向为干细胞治疗,Email: ylhmin@hotmail.com

  • 中图分类号: R617

Effect of human umbilical cord mesenchymal stem cells on CD4+ T cells in liver after hepatic ischemia-reperfusion injury in mice

More Information
  • 摘要:   目的  探讨人脐带间充质干细胞(HUC-MSCs)对小鼠肝缺血-再灌注损伤(HIRI)后肝内CD4+T细胞的影响。  方法  将225只小鼠随机分为sham组、control组和MSC组,每组75只。其中MSC组和control组均为HIRI模型小鼠,MSC组通过下腔静脉注射HUC-MSCs,control组通过下腔静脉注射生理盐水,sham组仅进行开腹、关腹等操作,不行血管夹闭。分别于术后6、12、24 h,每组随机取15只小鼠,用于眼球取血法采血及取肝组织样本,每组剩下的30只小鼠用于肝内单核细胞的提取。比较各组小鼠不同时间点肝内单核细胞数量,CD4+T细胞比例、数量和阳性率;比较各组小鼠不同时间点血清和肝组织中白细胞介素(IL)-17含量,及肝组织内共刺激分子B7-1和B7-2信使核糖核酸(mRNA)表达水平。  结果  术后12、24 h,control组的肝内单核细胞数量明显高于sham组,而MSC组的肝内单核细胞数量明显低于control组(P < 0.01~0.05)。术后6、12、24 h,control组的CD4+T细胞比例、数量及阳性率均明显高于sham组(均为P < 0.01),MSC组的CD4+T细胞比例明显低于control组(P < 0.01~0.05);术后12、24 h,MSC组的CD4+T细胞数量和阳性率均明显低于control组(P < 0.01~0.05)。术后6、12、24 h,control组血清和肝组织中的IL-17含量均高于sham组(均为P < 0.01),而MSC组血清和肝组织中的IL-17含量均低于control组(均为P < 0.01)。术后6 h,control组B7-2的mRNA表达水平高于sham组(P < 0.05);术后12、24 h,control组B7-1和B7-2的mRNA表达水平均高于sham组(均为P < 0.01),而MSC组B7-1和B7-2的mRNA表达水平均低于control组(均为P < 0.01)。  结论  HUC-MSCs抑制HIRI后肝内CD4+T细胞的数量和IL-17的分泌,同时减少肝内单核细胞数量及B7-1和B7-2 mRNA表达,减轻HIRI。

     

  • 图  1  3组小鼠肝内单核细胞和CD4+T细胞的数量和比例

    A图为3组小鼠肝内CD4+T细胞的流式细胞图;B图为3组小鼠肝脏单核细胞数量的比较;C图为3组小鼠CD4+T细胞比例的比较;D组为3组小鼠CD4+T细胞数量的比较;与sham组比较,aP<0.01;与control组比较,bP<0.05,cP<0.01

    Figure  1.  The counts and ratio of monocytes and CD4+ T cells in liver of mice of three groups

    图  2  3组小鼠肝内CD4+ T细胞的阳性率

    A图为3组小鼠肝内CD4+T细胞的免疫组化图片(×200),箭头所示为CD4+T细胞;B图为3组小鼠CD4+T细胞阳性率的比较,与sham组比较,aP<0.01;与control组比较,bP<0.05,cP<0.01

    Figure  2.  The positive rate of CD4+ T cells in liver of mice of three groups

    图  3  3组小鼠血清和肝组织中IL-17含量的比较

    与sham组比较,aP<0.01;与control组比较,bP<0.01

    Figure  3.  Comparison of the content of IL-17 in serum and liver tissue of mice among three groups

    图  4  3组小鼠肝组织中B7-1和B7-2 mRNA表达水平的比较

    A图为3组小鼠肝组织中B7-1 mRNA表达水平的比较;B图为3组小鼠肝组织中B7-2 mRNA表达水平的比较;与sham组比较,aP<0.01,bP<0.05;与control组比较,cP<0.01

    Figure  4.  Comparison of the expression levels of B7-1 and B7-2 mRNA in liver tissue of mice among three groups

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出版历程
  • 收稿日期:  2017-12-20
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2018-03-15

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