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人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究

白剑 肖漓 苗兰英 林大勇 刘鸿 高钰 陈文 毕丽丽 孔祥瑞 黄海燕 石炳毅

白剑, 肖漓, 苗兰英, 等. 人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究[J]. 器官移植, 2018, 9(2): 97-102. doi: 10.3969/j.issn.1674-7445.2018.02.002
引用本文: 白剑, 肖漓, 苗兰英, 等. 人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究[J]. 器官移植, 2018, 9(2): 97-102. doi: 10.3969/j.issn.1674-7445.2018.02.002
Bai Jian, Xiao Li, Miao Lanying, et al. Experimental study on the in vitro induction of regulatory T cells by umbilical cord mesenchymal stem cells with positive human leukocyte antigen-G[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 97-102. doi: 10.3969/j.issn.1674-7445.2018.02.002
Citation: Bai Jian, Xiao Li, Miao Lanying, et al. Experimental study on the in vitro induction of regulatory T cells by umbilical cord mesenchymal stem cells with positive human leukocyte antigen-G[J]. ORGAN TRANSPLANTATION, 2018, 9(2): 97-102. doi: 10.3969/j.issn.1674-7445.2018.02.002

人类白细胞抗原-G阳性的脐带间充质干细胞 体外诱导调节性T细胞的实验研究

doi: 10.3969/j.issn.1674-7445.2018.02.002
基金项目: 

国家自然基金面上项目 81571555

辽宁省自然科学基金 2015020714

详细信息
    作者简介:

    白剑,男,1979年生,博士,助理研究员,研究方向为免疫调节、免疫耐受,Email: baijian988@hotmail.com

    通讯作者:

    石炳毅,男,1953年生,博士,主任医师,博士研究生导师,研究方向为器官移植,Email: shibingyi@medmail.com.cn

  • 中图分类号: R392.4

Experimental study on the in vitro induction of regulatory T cells by umbilical cord mesenchymal stem cells with positive human leukocyte antigen-G

More Information
  • 摘要:   目的  探讨人类白细胞抗原(HLA)-G阳性的脐带间充质干细胞在体外诱导调节性T细胞(Treg)产生的效果。  方法  从新生儿脐带中分离脐带间充质干细胞,采用脂质体转染的方式将PEGFP-N1-HLA-G质粒转染到脐带间充质干细胞中,设为PEGFP-N1-HLA-G组; 转染空载体PEGFP-N1质粒的脐带间充质干细胞设为PEGFP-N1组; 相同条件下,未加入空载体的脐带间充质干细胞设为空白对照组。采用流式细胞仪检测脐带间充质干细胞标志物; 采用蛋白质免疫印迹法鉴定各组细胞HLA-G蛋白的表达; 各组细胞与健康人外周血中CD4+T细胞混合培养24 h和48 h后,采用流式细胞仪检测CD4+ CD25+ Foxp3+Treg占全部T细胞的比例。  结果  脐带间充质干细胞CD45、CD34和HLA-DR呈阴性表达,CD29、CD44和CD105呈阳性表达; PEGFP-N1-HLA-G组可以表达HLA-G蛋白,与空白对照组和PEGFP-N1组比较差异均有统计学意义(均为P < 0.01)。PEGFP-N1-HLA-G组细胞在与CD4+T细胞混合培养24 h后,CD4+ CD25+ Foxp3+Treg占全部T细胞的(15.3±1.9)%,在培养48 h后,CD4+ CD25+ Foxp3+Treg占全部T细胞的(14.3±2.1)%,与空白对照组和PEGFP-N1组比较,差异均有统计学意义(均为P < 0.05)。  结论  HLA-G基因修饰后脐带间充质干细胞能够有效地在体外诱导CD4+ CD25+ Foxp3+Treg的产生。

     

  • 图  1  脐带间充质干细胞标志物的表达情况

    Figure  1.  The expression of markers of umbilical cord mesenchymal stem cells

    图  2  各组脐带间充质干细胞HLA-G蛋白的表达情况

    与空白对照组比较,aP<0.01;与PEGFP-N1组比较,bP<0.01

    Figure  2.  Expression of HLA-G protein in umbilical cord mesenchymal stem cells of each group

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出版历程
  • 收稿日期:  2017-11-01
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2018-03-15

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