Relationship between the expression of SDF-1, ICAM-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury
-
摘要:
目的 探讨肾缺血-再灌注损伤(IRI)大鼠基质细胞衍生因子(SDF)-1、细胞间黏附分子-1(ICAM-1)与肾小管坏死评分的相关性。 方法 将60只SD大鼠随机分成手术组、假手术组两组,每组各30只。根据手术后检测时间不同,每组再分为6个不同时段的亚组(1、6、12、24、48、72 h组),每个亚组有5只大鼠。手术组建立大鼠肾IRI模型,假手术组大鼠仅予游离双侧肾动脉后缝合切口。检测各个时间点肾功能、肾小管坏死评分及肾脏组织SDF-1、ICAM-1表达变化。对手术组大鼠肾组织SDF-1、ICAM-1表达与肾功能和肾小管坏死评分进行Pearson直线相关分析。 结果 手术组术后血尿素氮(BUN)、血清肌酐(Scr)较术前及相应时间段假手术亚组明显升高(均为P<0.05),且于术后12 h显著升高,高峰期在术后48 h。手术组肾小管坏死评分随时间的延长逐渐增高(均为P<0.05);肾小管坏死评分最高在手术48 h组(P<0.05)。与手术1 h组比较,手术6 h组大鼠肾组织SDF-1、ICAM-1表达开始明显增多(均为P<0.05);手术后48 h达高峰,于手术后72 h开始下降。手术组的肾组织SDF-1、ICAM-1表达与术后各时间段BUN、Scr、肾小管坏死评分呈正相关(r=0.614、0.662、0.751;0.640、0.703、0.785;均为P<0.05)。 结论 当大鼠肾组织发生IRI时,SDF-1、ICAM-1表达上调,BUN、Scr升高,肾小管坏死评分升高,而且SDF-1、ICAM-1的表达与BUN、Scr、肾小管坏死评分呈正相关,提示SDF-1、ICAM-1表达增高程度可以作为反映肾IRI后严重程度的指标。 -
关键词:
- 大鼠 /
- 肾 /
- 缺血-再灌注损伤 /
- 基质细胞衍生因子-1 /
- 细胞间黏附分子-1
Abstract:Objective To investigate the relationship between the expression of stromal cell derived factor (SDF)-1, intercellular adhesion molecule (ICAM)-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury (IRI). Methods Sixty Sprague-Dawley (SD) rats were randomly divided into operation group and sham operation group with 30 rats in each group. Then each group was divided into 6 subgroups (1 h,6 h,12 h,24 h, 48 h or 72 h) according to different time of measurement after operation with 5 rats in each subgroup. Renal IRI mode1s were built in rats of operation group. The bilateral renal arteries were dissected in rats of sham operation group and then the incision was sutured. The renal function, renal tubular necrosis score and the variation of SDF-1, ICAM-1 expression in renal tissues at different time points were measured. The relationship between the expression of SDF-1, ICAM-1 in renal tissues and renal tubular necrosis score in rats of operation group was analyzed by Pearson correlation analysis. Results The levels of urea nitrogen (BUN) and serum creatinine (Scr) after operation in each subgroup of operation group were significantly higher than those before operation and those in the corresponding subgroups of sham operation group(all in P<0.05). They increased significantly 12 h after operation and reached the peak at 48 h after operation. The renal tubular necrosis score in operation group increased gradually over time (all in P<0.05). The highest renal tubular necrosis score was in the 48 h operation subgroup (P<0.05). Compared with those in 1 h operation subgroup, the expression of SDF-1, ICAM-1 in rats' renal tissues of 6 h operation subgroup began to increase significantly, and they reached the peak at 48 h after operation and began to drop down at 72 h after operation. The expression of SDF-1, ICAM-1 in rats renal tissues in operation group were positively correlated with the levels of BUN, Scr and renal tubular necrosis score at different time points after operation (r=0.614, 0.662, 0.751;0.640, 0.703, 0.785;P<0.05). Conclusion When rat's renal tissue develops IRI, the expression of SDF-1, ICAM-1, BUN, Scr and renal tubular necrosis score increased.The expression of SDF-1, ICAM-1 are positively correlated with BUN, Scr and renal tubular necrosis score. The increased expression of SDF-1, ICAM-1 can serve as an indicator of the severity of renal IRI. -
表 1 各组大鼠手术前后不同时间的肾功能变化
Table 1. The change of renal function in each group of rats at different time point before and after surgery (x±s)
分 组 n BUN(mmol/L) Scr(μmol/L) 术前 术后 术前 术后 手术组 1 h组 5 6.31±0.65 16.37±1.12a,b 46.92±2.93 109.27±10.12a,b 6 h组 5 6.57±0.54 19.78±3.18a,b 46.01±1.37 122.84±11.86a,b 12 h组 5 7.00±0.33 24.23±2.35a,b 49.00±5.09 216.10±17.24a,b 24 h组 5 7.33±0.34 39.92±3.00a,b 46.65±2.67 251.20±12.26a,b 48 h组 5 7.21±0.42 47.28±2.64a,b 46.54±3.80 307.00±12.01a,b 72 h组 5 7.45±0.32 25.35±4.48a,b 47.01±2.13 223.00±12.34a,b 假手术组 1 h组 5 6.17±0.10 6.02±0.24 47.29±1.60 50.29±4.56 6 h组 5 6.35±0.23 6.38±0.78 49.02±2.51 50.13±3.04 12 h组 5 7.64±0.12 6.43±0.42 47.83±2.62 51.67±5.95 24 h组 5 7.07±0.45 6.83±0.52 49.92±3.40 49.34±3.23 48 h组 5 7.55±0.35 7.13±0.64 46.50±1.23 46.17±2.17 72 h组 5 6.78±0.40 7.38±0.50 48.63±2.97 52.00±2.95 注:与同组术前比较,aP<0.05;与假手术同时间亚组比较,bP<0.05 表 2 各组大鼠手术后不同时间的SDF-1、ICAM-1的表达
Table 2. SDF-1 and ICAM-1 expressions in each group of rats at different timepoint after surgery (μg/ml,(x±s))
分 组 n SDF-1 ICAM-1 手术组 1 h组 5 0.86±0.26 0.92±0.31 6 h组 5 1.06±0.15a 1.28±0.23a 12 h组 5 1.36±0.30a 1.45±0.33a 24 h组 5 2.03±0.22a 1.73±0.21a 48 h组 5 3.35±0.13a 2.17±0.14a 72 h组 5 3.11±0.28a 2.13±0.24a 假手术组 1 h组 5 0.53±0.32 0.72±0.11 6 h组 5 0.72±0.25 0.81±0.23 12 h组 5 0.66±0.27 0.79±0.31 24 h组 5 0.59±0.22 0.83±0.16 48 h组 5 0.78±0.16 0.82±0.22 72 h组 5 0.53±0.32 0.77±0.12 注:与手术1 h组比较,aP<0.05 表 3 各组大鼠术后不同时间段肾小管坏死评分
Table 3. Tubular necrosis score in each group of rats at different timepoint after surgery (x±s
时 间 n 手术组 n 假手术组 肾小管坏死评分 肾小管坏死评分 1 h 5 42.27±2.82a 5 14.25±0.16 6 h 5 44.30±3.50a 5 15.30±0.56 12 h 5 62.50±1.87a 5 14.80±0.75 24 h 5 97.30±1.63a 5 15.30±0.82 48 h 5 148.70±5.43a 5 15.20±0.75 72 h 5 122.50±3.78a 5 14.50±1.05 注:与假手术组同时间亚组比较,aP<0.05 -
[1] Kłoda K, Domański L, Pawlik A, et al. The impact of ICAM1 and VCAM1 gene polymorphisms on chronic allograft nephropathy and transplanted kidney function[J]. Transplant Proc,2013,45(6):2244-2247. doi: 10.1016/j.transproceed.2013.03.043 [2] Kłoda K, Domański L, Pawlik A, et al. The impact of ICAM1 and VCAM1 gene polymorphisms on long-term renal transplant function and recipient outcomes[J]. Ann Transplant,2013,18:231-237. doi: 10.12659/AOT.883917 [3] Mannam VK, Lewis RE, Cruse JM. The fate of renal allografts hinges on responses of the microvascular endothelium[J]. Exp Mol Pathol,2013,94(2):398-411. doi: 10.1016/j.yexmp.2012.06.002 [4] Cho HK, Kim SY, Seong JK, et al. Hepatitis B virus X increases immune cell recruitment by induction of chemokine SDF-1[J]. FEBS Lett,2014,588(5):733-739. doi: 10.1016/j.febslet.2014.01.017 [5] Flameng W, De Visscher G, Mesure L, et al. Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricularoutflow tract reconstruction eliminates immune response-related degeneration[J]. J Thorac Cardiovasc Surg,2014,147(4):1398-1404. doi: 10.1016/j.jtcvs.2013.06.022 [6] 易小敏, 张更, 马帅军. 大鼠急性肾缺血再灌注损伤模型的建立与评估[J]. 现代生物医学进展,2011, 11(21): 4027-4029. http://www.cnki.com.cn/Article/CJFDTOTAL-SWCX201121008.htmYi XM, Zhang G, Ma SJ. Establishment and evaluation of rat acute kidney ischemia/reperfusion model[J]. Prog Mod Biomed,2011, 11(21): 4027-4029. http://www.cnki.com.cn/Article/CJFDTOTAL-SWCX201121008.htm [7] Paller MS, Hoidal JR, Ferris TF. Oxygen free radicals in ischemic acute renal failure in the rat[J]. J Clin Invest,1984,74(4):1156-1164. doi: 10.1172/JCI111524 [8] Shayegi N, Meyer C, Lambert K, et al. CXCR4 blockade and sphingosine-1-phosphate activation facilitate engraftment of haematopoietic stem and progenitor cells in a non-myeloablative transplant model[J]. Br J Haematol,2014,164(3):409-413. doi: 10.1111/bjh.2014.164.issue-3 [9] Yang D, Sun S, Wang Z, et al. Stromal cell-derived factor-1 receptor CXCR4-overexpressing bone marrow mesenchymal stem cells accelerate wound healing by migrating into skin injury areas[J]. Cell Reprogram,2013,15(3):206-215. http://cn.bing.com/academic/profile?id=65100295&encoded=0&v=paper_preview&mkt=zh-cn [10] Xu X, Zhu F, Zhang M, et al. Stromal cell-derived factor-1 enhances wound healing through recruiting bone marrow-derived mesenchymalstem cells to the wound area and promoting neovascularization[J]. Cells Tissues Organs,2013,197(2):103-113. doi: 10.1159/000342921 [11] Xargay-Torrent S, López-Guerra M, Montraveta A, et al. Sorafenib inhibits cell migration and stroma-mediated bortezomib resistance by interfering B-cell receptor signaling and protein translation in mantle cell lymphoma[J]. Clin Cancer Res,2013,19(3):586-597. doi: 10.1158/1078-0432.CCR-12-1935 [12] Wang S, Cheng H, Dai G, et al. Umbilical cord mesenchymal stem cell transplantation significantly improves neurological function in patients with sequelae of traumatic brain injury[J]. Brain Res,2013,1532:76-84. doi: 10.1016/j.brainres.2013.08.001 [13] Wang L, Pasha Z, Wang S, et al. Protein kinase G1α overexpression increases stem cell survival and cardiac function after myocardial infarction[J]. PLoS One,2013,8(3):e60087. doi: 10.1371/journal.pone.0060087 [14] Virani S, Edwards AK, Thomas R, et al. Blocking of stromal cell-derived factor-1 reduces neoangiogenesis in human endometriosis lesions in a mouse model[J]. Am J Reprod Immunol,2013,70(5):386-397. http://cn.bing.com/academic/profile?id=2064946686&encoded=0&v=paper_preview&mkt=zh-cn [15] Tadakuma K, Tanaka N, Haraguchi Y, et al. A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage[J]. Biomaterials,2013,34(36):9018-9025. doi: 10.1016/j.biomaterials.2013.08.006 [16] Sun D, Narsinh K, Wang H, et al. Effect of autologous bone marrow mononuclear cells transplantation in diabetic patients with ST-segmentelevation myocardial infarction[J]. Int J Cardiol,2013,167(2):537-547. doi: 10.1016/j.ijcard.2012.01.068 [17] Suga A, Ueda K, Takemoto Y, et al. Significant role of bone marrow-derived cells in compensatory regenerative lung growth[J]. J Surg Res,2013,183(1):84-90. doi: 10.1016/j.jss.2012.12.013 [18] Stojanovic D, Cvetkovic T, Stojanovic M, et al. Crosstalk of inflammatory mediators and lipid parameters as early markers of renal dysfunction in stable renal transplant recipients with regard to immunosuppression[J]. Ann Transplant,2013,18:414-423. doi: 10.12659/AOT.889239 [19] 张力,赵雪云,刘胜春,等. 蛋白酶体抑制剂MG132减轻大鼠移植胰腺缺血再灌注损伤的作用[J].中华器官移植杂志,2013,34(6):358-361.Zhang L, Zhao XY, Liu SC, et al. Protective effect of MG132 on ischemia-reperfusion injury after pancreaticoduodenal transplantation in rats[J]. Chin J Organ Transplant,2013,34(6):358-361. [20] 王烨铭,陈静瑜,童继春,等.乌司他丁减轻无心跳大鼠供肺缺血再灌注损伤的作用及机制[J].中华器官移植杂志,2013,34(8):498-502.Wang YM, Chen JY, Tong JC, et al. Protective effects of ulinastatin on ischemia-reperfusion injury during rat non-heart beating donor lung transplantation[J]. Chin J Organ Transplant,2013,34(8):498-502.
计量
- 文章访问数: 108
- HTML全文浏览量: 49
- PDF下载量: 104
- 被引次数: 0