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治疗性高碳酸血症对肺移植缺血-再灌注损伤早期炎症因子的影响

王玲 拉佈旦白拉

王玲, 拉佈旦白拉. 治疗性高碳酸血症对肺移植缺血-再灌注损伤早期炎症因子的影响[J]. 器官移植, 2014, 5(1): 8-11. doi: 10.3969/j.issn.1674-7445.2014.01.003
引用本文: 王玲, 拉佈旦白拉. 治疗性高碳酸血症对肺移植缺血-再灌注损伤早期炎症因子的影响[J]. 器官移植, 2014, 5(1): 8-11. doi: 10.3969/j.issn.1674-7445.2014.01.003
Wang Ling, La Budanbaila. Effect of therapeutic hypercapnia on the early inflammatory factors after ischemia-reperfusion injury of lung transplantation[J]. ORGAN TRANSPLANTATION, 2014, 5(1): 8-11. doi: 10.3969/j.issn.1674-7445.2014.01.003
Citation: Wang Ling, La Budanbaila. Effect of therapeutic hypercapnia on the early inflammatory factors after ischemia-reperfusion injury of lung transplantation[J]. ORGAN TRANSPLANTATION, 2014, 5(1): 8-11. doi: 10.3969/j.issn.1674-7445.2014.01.003

治疗性高碳酸血症对肺移植缺血-再灌注损伤早期炎症因子的影响

doi: 10.3969/j.issn.1674-7445.2014.01.003
详细信息
    通讯作者:

    拉佈旦白拉,Email:labudan@163.com

  • 中图分类号: R617

Effect of therapeutic hypercapnia on the early inflammatory factors after ischemia-reperfusion injury of lung transplantation

  • 摘要:   目的   研究治疗性高碳酸血症对肺移植缺血-再灌注早期炎症因子的影响。   方 法   72只Wistar雄性大鼠,随机分为模型组(36只)和治疗组(36只)。两组基础状态给予50% N2+50% O2通气,模型组移植成功后给予50% N2+50% O2通气;治疗组移植成功后给予50% O2、8%CO2和42% N2通气,维持动脉血二氧化碳分压(PaCO2)在80~100 mmHg(10 mmHg=1.33 kPa)之间。记录受体大鼠机械通气1 min时MAP、PaCO2和动脉血氧分压(PaO2)作为基础值,再灌注期间每30 min记录1次,直至实验结束。分别于再灌注1、2、4 h取左肺下叶组织,采用酶联免疫吸附法测定肺组织中肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β的水平。   结果   与模型组比较,再灌注后各时间点治疗组受体鼠的MAP、PaO2均明显升高(均为P<0.05)。与模型组比较,治疗组各时间点移植肺组织中TNF-α和IL-1β水平均有显著下降(均为P<0.05)。   结论   治疗性高碳酸血症对肺移植缺血-再灌注损伤后早期炎症因子的释放有抑制作用。

     

  • 表  1  两组受体鼠再灌注后不同时间点平均动脉压与血气分析结果的比较

    Table  1.   Comparison of mean arterial pressure and arterial blood gas analysis of recipient rats between two groups at different time points after reperfusion (mmHg,x±s)

    项目 n 基础值 再灌注
    30 min 60 min 90 min 120 min 150 min 180 min 210 min 240 min
    MAP
    治疗组模型组 1212 110±10115±8 112±8b95±10a 113±12b97±8a 118±10b100±7a 117±12b102±11a 114±12b106±12a 112±10b103±13a 114±12b100±11a 112±14b99±11a
    PaO2
    治疗组模型组1212234±19231±15218±36b185±30a225±30b186±26a 232±32b183±30a240±38b178±38a242±33b176±40a245±32b174±42a 241±35b170±36a247±31b168±42a
    PaCO2
    治疗组模型组121231±434±382±5a,b37±484±7a,b39±590±8a,b42±687±5a,b40±589±8a,b44±3 88±6a,b46±485±5a,b43±482±7a,b42±5
    注:与同组基础值比较,aP<0.05;与模型组比较,bP<0.05
    下载: 导出CSV

    表  2  两组受体鼠再灌注后移植肺组织炎症因子的比较

    Table  2.   Comparison of inflammatory factors of transplant lung tissues of recipient rats between two groups after reperfusion (μg/L,x±s)

    组别 n TNF-α IL-1β
    再灌注1 h再灌注2 h再灌注4 h 再灌注1 h再灌注2 h再灌注4 h
    治疗组120.50±0.11a0.62±0.06a0.77±0.14a0.28±0.03a0.33±0.06a0.31±0.05a
    模型组120.76±0.050.82±0.050.89±0.160.32±0.050.37±0.050.33±0.07
    注:与模型组比较,aP<0.05
    下载: 导出CSV
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出版历程
  • 收稿日期:  2013-10-31
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2014-01-15

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