Objective   To compare the change features of anti-donor specific antibody (DSA) in different species of sentitized mice after skin transplantation.

  Methods   All mice were divided into the Balb/c→C57BL/6 (6 pairs) and Balb/c→C3H skin transplantation groups (6 pairs). At d0, d2, d4, d7, d13, d17, d28, d35, d42, d49 and d56 after skin transplantation, the serum sample was prepared for detection of DSA-IgG and DSA-IgM levels.

  Results   Moderate increase was noted in the DSA-IgG level in the sensitized mice within 1 week after skin transplantation. The IgG level was significantly increased within 1-4 week and peaked and stabilized within 4-8 week. No significant variation was observed in the DSA-IgM level at 8 weeks after skin transplantation. In the Balb/c→C57BL/6 skin transplantation group, the DSA-IgG level was significantly lower than that in the Balb/c→C3H group. Statistical significance was identified in the IgG levels between two groups at d2, d17, d28, d35, d42, d49 and d56 after skin transplantation (all P < 0.05). No statistical significance was noted in the DSA-IgM levels between two groups at each time point (all P>0.05).

  Conclusion   Advancing the time of renal transplantation after skin transplantation moderately in the Balb/c→C3H group, or changing to the lower immunoreactive combination of Balb/c→C57BL/6 are aimed to establish AMR mouse models with mild rejection reaction.