Abstract: Lung transplant biological sample is the important human biological resource in the basic research of organ transplantation. It plays a key role in revealing the mechanisms of pulmonary disease development, studying post-transplant complications, and promoting clinical translation. The construction of high quality biological banks is crucial for the in-depth development of basic and clinical research related to transplantation. However, the development of lung transplant biological banks both domestically and internationally lacks unified standards and norms currently, which limits the full scientific value of sample resources and their cross-institutional sharing. Therefore, Branch of Organ Transplantation of Chinese Medical Association has organized multidisciplinary experts to jointly develop this guideline, which systematically proposes the core principles and operational standards for the construction of lung transplant biological banks. It aims to comprehensively guide the standardized construction of lung transplant biological banks and to strengthen the standardized protection and efficient sharing of biological information resources. The introduction of this guideline may provide important technical support and implementation basis for the standardized development of lung transplant biological banks in China, and further promote multi-center collaboration and the integration and utilization of lung transplant biomedical resources.