Research report of living donor kidney harvesting in Bama miniature pigs with six gene modified

Xu Yong; Song Xiangyu; Wang Heng’en; Yang Shujun; Jia Zhibo; Wei Hao; Chen Shengfeng; Cui Mengyi; Ren Yanling; Peng Jiang; Sun Shengkun

doi:10.3969/j.issn.1674-7445.2023215

Volume 15 Issue 2

Mar. 2024

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Article Navigation > ORGAN TRANSPLANTATION > 2024 > 15(2): 229-235

Xu Yong, Song Xiangyu, Wang Heng’en, et al. Research report of living donor kidney harvesting in Bama miniature pigs with six gene modified[J]. ORGAN TRANSPLANTATION, 2024, 15(2): 229-235. doi: 10.3969/j.issn.1674-7445.2023215

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Research report of living donor kidney harvesting in Bama miniature pigs with six gene modified

doi: 10.3969/j.issn.1674-7445.2023215

1.
Department of Urology, the Third Medical Center of Chinese People’s Liberation Army General Hospital, Beijing 100000, China

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Corresponding author: Sun Shengkun , Email:sunshengkun@301hospital.com.cn
Received Date: 2023-10-14
Accepted Date: 2023-12-06

Available Online: 2024-01-17

Publish Date: 2024-03-15

Abstract

Abstract

Objective To summarize the experience and practical value of living donor kidney harvesting in Bama miniature pigs with six gene modified. Methods The left kidney of Bama miniature pigs with six gene modified was obtained by living donor kidney harvesting technique. First, the ureter was occluded, and then the inferior vena cava and abdominal aorta were freed. During the harvesting process, the ureter, renal vein and renal artery were exposed and freed in sequence. The vascular forceps were used at the abdominal aorta and inferior vena cava, and the renal artery and vein were immediately perfused with 4℃ renal preservation solution, and stored in ice normal saline for subsequent transplantation. Simultaneously, the donor abdominal aorta and inferior vena cava gap were sutured. The operation time, blood loss, warm and cold ischemia time, postoperative complications and the survival of donors and recipients were recorded. Results The left kidney of the genetically modified pig was successfully harvested. Intraoperative bleeding was 5 mL, warm ischemia time was 45 s, and cold ischemia time was 2.5 h. Neither donor nor recipient pig received blood transfusion, and urinary function of the kidney transplanted into the recipient was recovered. The donor survived for more than 8 months after the left kidney was resected. Conclusions Living donor kidney harvesting is safe and reliable in genetically modified pigs. Branch blood vessels could be processed during kidney harvesting, which shortens the process of kidney repair and the time of cold ischemia. Living donor kidney harvesting contributes to subsequent survival of donors and other scientific researches.
- Xenotransplantation,
- Living donor kidney,
- Nephrectomy,
- End-stage renal disease,
- Kidney transplantation,
- Donor kidney procurement,
- Genetically modified pig,
- Minimally invasive technique

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References

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Research report of living donor kidney harvesting in Bama miniature pigs with six gene modified

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