Volume 11 Issue 6
Jan.  2021
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Article Navigation >  ORGAN TRANSPLANTATION  > 2020  >  11(6): 692-697
Wang Li, Chen Xiaolong, Liu Huiling, et al. β-arrestin-2 inhibits autophagy by up-regulating PI3K/Akt signal to alleviate liver ischemia-reperfusion injury in mice[J]. ORGAN TRANSPLANTATION, 2020, 11(6): 692-697. doi: 10.3969/j.issn.1674-7445.2020.06.007
Citation: Wang Li, Chen Xiaolong, Liu Huiling, et al. β-arrestin-2 inhibits autophagy by up-regulating PI3K/Akt signal to alleviate liver ischemia-reperfusion injury in mice[J]. ORGAN TRANSPLANTATION, 2020, 11(6): 692-697. doi: 10.3969/j.issn.1674-7445.2020.06.007
shu

β-arrestin-2 inhibits autophagy by up-regulating PI3K/Akt signal to alleviate liver ischemia-reperfusion injury in mice

doi: 10.3969/j.issn.1674-7445.2020.06.007

  • Department of Hepatic Surgery, Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China

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  • Corresponding author: Wang Genshu, Email: wgsh168@163.com
  • Received Date: 2020-08-03
    Available Online: 2021-01-19
  • Publish Date: 2021-01-19
    Abstract
  •   Objective  To verify whether β-arrestin-2 inhibits autophagy by up-regulating PI3K/Akt signal to protect the liver from ischemia-reperfusion injury (IRI) in mice.  Methods  Twelve β-arrestin-2 knockout (KO) and twelve wild-type (WT) C57BL/6 mice were randomly divided into the KO+sham group, KO+IRI group, WT+sham group and WT+IRI group, six mice in each group. The mouse models with 70% liver IRI were established or sham operation was performed. Relevant experiments were carried out at 6 h after liver reperfusion or operation. The expression levels of apoptosis signal protein cleaved Caspase-3, proliferation signal protein Ki-67 and the PI3K/Akt signal protein p-Akt were detected by immunohistochemical staining.  Results  Immunohistochemical staining demonstrated that compared with the corresponding sham group, the positive cell count for cleaved Caspase-3, Ki-67 and p-Akt in liver tissues of mice was significantly increased in the KO+IRI and WT+IRI groups (all P < 0.01). Compared with the WT+IRI group, the positive cell count for cleaved Caspase-3 in liver tissues of mice was significantly increased, whereas the positive cell count forKi-67 and p-Akt was significantly decreased in the KO+IRI group (both P < 0.05).  Conclusions  β-arrestin-2 can mitigate the liver cell apoptosis and promote the repair of injury after IRI in mice. Moreover, β-arrestin-2 inhibits autophagy by up-regulating the PI3K/Akt signal to alleviate liver IRI in mice.

     

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