Screening and bioinformatics analysis of key autophagy-related genes in alcoholic hepatitis

Yuan Chao; Lian Qinghai; Ni Beibei; Xu Yan; Zhang Tong; Zhang Jian

doi:10.3969/j.issn.1674-7445.2023163

Volume 15 Issue 1

Jan. 2024

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Yuan Chao, Lian Qinghai, Ni Beibei, et al. Screening and bioinformatics analysis of key autophagy-related genes in alcoholic hepatitis[J]. ORGAN TRANSPLANTATION, 2024, 15(1): 90-101. doi: 10.3969/j.issn.1674-7445.2023163

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Screening and bioinformatics analysis of key autophagy-related genes in alcoholic hepatitis

doi: 10.3969/j.issn.1674-7445.2023163

1.
Department of Hepatic Surgery, Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China

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Corresponding author: Zhang Tong, Email: zhjeff72@sina.com
Received Date: 2023-08-24
Accepted Date: 2023-11-10

Available Online: 2023-11-29

Publish Date: 2024-01-11

Abstract

Abstract

Objective To screen key autophagy-related genes in alcoholic hepatitis (AH) and investigate potential biomarkers and therapeutic targets for AH. Methods Two AH gene chips in Gene Expression Omnibus (GEO) and autophagy-related data sets obtained from MSigDB and GeneCards databases were used, and the key genes were verified and obtained by weighted gene co-expression network analysis (WGCNA). The screened key genes were subject to gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interaction (PPI) and immune infiltration analyses. Messenger RNA (mRNA)- microRNA (miRNA) network was constructed to analyze the expression differences of key autophagy-related genes during different stages of AH, which were further validated by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) in the liver tissues of AH patients and mice. Results Eleven autophagy-related genes were screened in AH (EEF1A2, CFTR, SOX4, TREM2, CTHRC1, HSPB8, TUBB3, PRKAA2, RNASE1, MTCL1 and HGF), all of which were up-regulated. In the liver tissues of AH patients and mice, the relative expression levels of SOX4, TREM2, HSPB8 and PRKAA2 in the AH group were higher than those in the control group. Conclusions SOX4, TREM2, HSPB8 and PRKAA2 may be potential biomarkers and therapeutic targets for AH.

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Screening and bioinformatics analysis of key autophagy-related genes in alcoholic hepatitis

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Screening and bioinformatics analysis of key autophagy-related genes in alcoholic hepatitis

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