Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

Luo De; Liu Jiang; Zhou Pengcheng; Wang Piao; Li Xujia; Lin Haomin; Su Song

doi:10.3969/j.issn.1674-7445.2023132

Volume 14 Issue 6

Nov. 2023

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Luo De, Liu Jiang, Zhou Pengcheng, et al. Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney[J]. ORGAN TRANSPLANTATION, 2023, 14(6): 824-830. doi: 10.3969/j.issn.1674-7445.2023132

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Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

doi: 10.3969/j.issn.1674-7445.2023132

1.
Department of Hepatobiliary Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China

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Corresponding author: Su Song, Email: 13882778554@163.com
Received Date: 2023-07-05
Accepted Date: 2023-09-11

Available Online: 2023-09-19

Publish Date: 2023-11-09

Abstract

Abstract

Objective To evaluate the effect of hypothermic machine perfusion (HMP) on the expression levels of inflammatory cytokines in rat kidney. Methods Thirty male rats were randomly divided into the control (Control group), static cold storage group (SCS group) and HMP group, with 10 rats in each group. The velocity, intrarenal resistance and pH value of perfusion effluent were recorded during HMP. The expression levels of CXC chemokine ligand (CXCL)1, CXCL2, interferon (IFN)-β1, IFN-α4, CC chemokine ligand (CCL)2, CCL20, interleukin (IL)-17α, IL-17C and tumor necrosis factor (TNF)-α messenger RNA (mRNA) in renal tissues were evaluated by reverse transcription polymerase chain reaction (RT-PCR). Pathological changes of the kidney were observed by hematoxylin-eosin (HE) staining. Results During HMP, the velocity and intrarenal resistance remained stable, and the pH value of perfusion effluent was decreased slowly. RT-PCR showed that the relative expression levels of CXCL1, CXCL2, CCL2, CCL20, IL-17α, IL-17C and TNF-α mRNA in the SCS and HMP groups were higher compared with those in the Control group. Compared with the SCS group, the relative expression levels of CXCL1, CXCL2, CCL2, CCL20, IL-17α and TNF-α mRNA were up-regulated in the HMP group (all P<0.05). HE staining revealed that the morphology of renal cells was normal in the Control group, whereas evident epithelial necrosis, cytoplasmic vacuolation, brush border loss and epithelial shedding were observed in the SCS group. Compared with the SCS group, pathological changes in the HMP group were alleviated. Conclusions HMP may activate renal inflammation, and inhibiting the activation of inflammation during HMP is expected to further improve the effect of allograft preservation.
- Kidney transplantation,
- Hypothermic mechanical perfusion,
- Static cold storage,
- Inflammatory factor,
- Interleukin,
- Interferon,
- Tumor necrosis factor,
- Chemokine

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Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

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Effect of hypothermic machine perfusion on expression levels of inflammatory cytokines in rat kidney

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