Volume 12 Issue 1
Jan.  2021
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Cheng Haoyu, Yang Fan, Yang Yixin, et al. Application of quantitative detection of multiple-source cytomegalovirus DNA in diagnosis of cytomegalovirus pneumonia after allogeneic hematopoietic stem cell transplantation[J]. ORGAN TRANSPLANTATION, 2021, 12(1): 96-102. doi: 10.3969/j.issn.1674-7445.2021.01.015
Citation: Cheng Haoyu, Yang Fan, Yang Yixin, et al. Application of quantitative detection of multiple-source cytomegalovirus DNA in diagnosis of cytomegalovirus pneumonia after allogeneic hematopoietic stem cell transplantation[J]. ORGAN TRANSPLANTATION, 2021, 12(1): 96-102. doi: 10.3969/j.issn.1674-7445.2021.01.015
shu

Application of quantitative detection of multiple-source cytomegalovirus DNA in diagnosis of cytomegalovirus pneumonia after allogeneic hematopoietic stem cell transplantation

doi: 10.3969/j.issn.1674-7445.2021.01.015

  • Department of Hematology, Aerospace Center Hospital, Beijing 100049, China

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  • Corresponding author: Wang Jingbo, E-mail:dpwangjingbo@vip.sina.com
  • Received Date: 2020-09-20
    Available Online: 2021-01-19
  • Publish Date: 2021-01-19
    Abstract
  •   Objective  To evaluate the diagnostic value of quantitative detection of cytomegalovirus (CMV) DNA from different sources [plasma, sputum and bronchoalveolar lavage fluid(BALF)] for CMV pneumonia after allogeneic hematopoietic stem cell transplantation.  Methods  Clinical data of 405 recipients undergoing allogeneic hematopoietic stem cell transplantation were retrospectively analyzed. Among them, 19 recipients diagnosed with CMV pneumonia were assigned into the CMV pneumonia group, and 229 recipients with CMV viremia alone, 11 recipients without CMV pneumonia who received fiberoptic bronchoscopy and 16 recipients diagnosed with bacterial or fungal pneumonia based on pathogenic evidence receiving sputum culture were assigned into the control A, B and C groups, respectively. The incidence of CMV pneumonia was summarized. The CMV DNA load of specimens from different sources (plasma, sputum and BALF) of recipients with CMV pneumonia was analyzed. The clinical prognosis of recipients with CMV pneumonia was evaluated.  Results  Among 405 recipients undergoing allogeneic hematopoietic stem cell transplantation, 19 cases developed CMV pneumonia, and the overall incidence of CMV pneumonia was 4.7%(19/405). The CMV DNA load in the plasma, sputum and BALF of recipients with CMV pneumonia was higher than those in the control A, B and C groups (all P < 0.05). In the 19 recipients, 12 cases were cured after antiviral treatment and 7 died from treatment failure(3 cases abandoned treatment). The fatality was 37%(7/19).  Conclusions  Quantitative detection of CMV DNA in the plasma, sputum and BALF may increase the diagnostic rate of CMV pneumonia, thereby improving clinical prognosis of recipients undergoing allogeneic hematopoietic stem cell transplantation.

     

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