Analysis on dynamic changes of T lymphocyte subsets in recipients with stable graft status after lung transplantation

Lian Qiaoyan; Chen Ao; Xu Xin; Wei Bing; Cai Yuhang; Huang Danxia; He Jianxing; Ju Chunrong

doi:10.3969/j.issn.1674-7445.2021.01.013

Volume 12 Issue 1

Jan. 2021

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Lian Qiaoyan, Chen Ao, Xu Xin, et al. Analysis on dynamic changes of T lymphocyte subsets in recipients with stable graft status after lung transplantation[J]. ORGAN TRANSPLANTATION, 2021, 12(1): 83-89. doi: 10.3969/j.issn.1674-7445.2021.01.013

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Analysis on dynamic changes of T lymphocyte subsets in recipients with stable graft status after lung transplantation

doi: 10.3969/j.issn.1674-7445.2021.01.013

Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510012, China

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Corresponding author: Ju Chunrong, E-mail:juchunrong@126.com
Received Date: 2020-09-06
Available Online: 2021-01-19
Publish Date: 2021-01-19

Abstract

Abstract

Objective To analyze the dynamic changes and the influencing factors of T lymphocyte subsets in recipients with stable graft status within 1 year after lung transplantation. Methods Clinical data of 41 recipients with stable graft status after allogeneic lung transplantation were analyzed. The absolute value and ratio of T lymphocyte subsets in peripheral blood from recipients were measured by flow cytometry before operation, 2 weeks and each month (within 1 year) after operation, respectively. The effects of age, gender, body mass index (BMI), surgical method, incidence of primary graft dysfunction (PGD) after operation, and primary disease upon the absolute values of T lymphocytes were evaluated. Results Within 1 year after lung transplantation, the absolute values of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺T lymphocytes and CD4⁺/CD8⁺ ratio were changed over time (all P < 0.001). Compared with preoperative values, there was no statistical significance in the absolute values of CD3⁺ and CD3⁺CD4⁺T lymphocytes at 12 months after operation (P=0.659, 0.109), whereas the absolute value of CD3⁺CD8⁺T lymphocytes was increased (P=0.02) and the CD4⁺/CD8⁺ ratio was decreased (P < 0.001). Age, gender, BMI, surgical method and incidence of PGD after operation exerted no significant effect on the dynamic changes of absolute values of CD3⁺CD4⁺ and CD3⁺CD8⁺T lymphocytes (all P > 0.05). Primary disease before lung transplantation exerted no effect on the changes of CD3⁺CD4⁺T lymphocytes, whereas the postoperative absolute value of CD3⁺CD8⁺T lymphocytes was higher in recipients with infectious lung diseases (P < 0.05). Conclusions The absolute values of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺T lymphocytes in recipients with stable graft status after lung transplantation are relatively low in the early stage after lung transplantation, then gradually restore, and stabilize at 6 months after operation. Dynamic changes are not associated with age, gender, BMI, surgical method and incidence of PGD after operation of recipients.
- Lung transplantation,
- T lymphocyte subset,
- CD3,
- CD4,
- CD8,
- Immunosuppressant,
- Rejection,
- Infection

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References

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Analysis on dynamic changes of T lymphocyte subsets in recipients with stable graft status after lung transplantation

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