Effect and mechanism of human gingival mesenchymal stem cell on B cells

Zhang Kai; Chen Keyan; Li Kai; Ma Tieliang; Gu Jian

doi:10.3969/j.issn.1674-7445.2020.02.011

Volume 11 Issue 2

Mar. 2020

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Zhang Kai, Chen Keyan, Li Kai, et al. Effect and mechanism of human gingival mesenchymal stem cell on B cells[J]. ORGAN TRANSPLANTATION, 2020, 11(2): 253-258. doi: 10.3969/j.issn.1674-7445.2020.02.011

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Effect and mechanism of human gingival mesenchymal stem cell on B cells

doi: 10.3969/j.issn.1674-7445.2020.02.011

Department of Hepatobiliary and Laparoscopic Surgery, Yixing Hospital affiliated to Jiangsu University, Yixing 214200, China

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Corresponding author: Zhang Kai, Email: zk_104@163.com
Received Date: 2020-01-14
Available Online: 2021-01-19
Publish Date: 2020-03-15

Abstract

Abstract

Objective To investigate the regulating function of human gingival mesenchymal stem cell (GMSC) on the proliferation and differentiation of B cells and its underlying molecular mechanism. Methods GMSC were isolated and B cells were isolated from peripheral blood. GMSC or fibroblasts were co-cultured with B cells in vitro and assigned into the GMSC group and fibroblast group. The proliferation of B cells was detected in two groups. The expression of IgG1 and IgM in the cell supernatants was measured between two groups. The secretion of interleukin (IL)-6, Perforin, interferon (IFN)-γ and tumor necrosis factor (TNF)-α was compared between two groups. The expression levels ofIL-10 and transforming growth factor (TGF)-β in B cells were detected between two groups. The expression of PC-1 in B cells was measured in two groups. The signaling pathway involved with the regulating effect of GMSC on B cell function was investigated. The regulating effect of GMSC on the role of B cells in activating T cell function was assessed. Results Compared with the fibroblast group, the proliferation of B cells was significantly weakened in the GMSC group (P < 0.05). Co-culture of GMSC and B cells significantly inhibited the secretion of IgG1 and IgM from B cells and the secretion ofIL-6, Perforin, IFN-γ and TNF-α (all P < 0.05). Compared with the fibroblast group, the secretion of IL-10 and TGF-βwas significantly higher in the GMSC group (both P < 0.05). The expression level of PC-1 in the GMSC group was significantly down-regulated (P < 0.05). After adding ALK5, an inhibitor of TGF-β receptor, the inhibitory effect of GMSC upon B cells was significantly weakened (P < 0.05). Compared with the fibroblast group, the ability of B cells to activate and proliferate T cells was significantly attenuated in the GMSC group (P < 0.05). Conclusions GMSC can inhibit B cells and their mediated immune responses. The activation of B cells and other related functions can be suppressed through the TGF-β signaling pathway.
- Transplantation immunity,
- Chronic graft-versus-host disease,
- Gingival mesenchymal stem cell,
- Human peripheral blood mononuclear cell,
- B cell,
- T cell,
- Regulatory B cell,
- Transforming growth factor

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Effect and mechanism of human gingival mesenchymal stem cell on B cells

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