Volume 9 Issue 6
Nov.  2018
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Li Xuemei, Gu Baohong, Hu Jike, et al. Effect and mechanism research of selective deletion of Ndst1 in donor kidney on acute rejection after renal transplantation in mouse models[J]. ORGAN TRANSPLANTATION, 2018, 9(6): 424-429. doi: 10.3969/j.issn.1674-7445.2018.06.005
Citation: Li Xuemei, Gu Baohong, Hu Jike, et al. Effect and mechanism research of selective deletion of Ndst1 in donor kidney on acute rejection after renal transplantation in mouse models[J]. ORGAN TRANSPLANTATION, 2018, 9(6): 424-429. doi: 10.3969/j.issn.1674-7445.2018.06.005
shu

Effect and mechanism research of selective deletion of Ndst1 in donor kidney on acute rejection after renal transplantation in mouse models

doi: 10.3969/j.issn.1674-7445.2018.06.005

  • The Second Clinical Medical College, Lanzhou University, Lanzhou 730000, China

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  • Corresponding author: Chen Hao, Email:chenhaodrs@163.com
  • Received Date: 2018-09-20
    Available Online: 2021-01-19
  • Publish Date: 2018-11-15
    Abstract
  •   Objective  To investigate the effect and mechanism of selective deletion of N-deacetylase-N-sulfotransferase (Ndst) 1 in donor kidney on the acute rejection after renal transplantation in the recipient mice.  Methods  A mouse model undergoing renal transplantation was established. The mice were divided into 3 groups according to the strains of mice and treatment: wild type (WT) group (n=10), Ndst1-/- group (n=9) and myxoma virus T7 protein(M-T 7)treatment group (n=7). The mice were euthanized at postoperative 10 d. Pathological examination and the content of disaccharide [heparan sulfate (HS) and chondroitin sulfate (CS)] in glycosaminoglycans (GAGs) were analyzed. The correlation between pathological score and changes of disaccharide content was analyzed.  Results  Compared with the WT group, the overall pathological scores of early rejection of the transplanted kidney tissues were significantly lower in the Ndst1-/- and M-T7 treatment groups (both P < 0.05). Compared with the WT group, the percentage of 6-O sulfation and the content of D0S6 in HS were significantly increased, whereas the contents of D0A4 and D2A4 in CS were significantly decreased in the transplanted kidney tissues in the Ndst1-/- group (all P < 0.05). Compared with the WT group, the percentage of 6-O sulfation in HS, the percentages of 6-O sulfation and 2-O sulfation in CS and the contents of D0S6 and D2S6 in HS were significantly increased, whereas the content of D2A0 in HS, the contents of D0A4 and D2A4 in CS were significantly decreased in the transplanted kidney tissues in the M-T7 treatment group (all P < 0.05). The changes of disaccharide content in the transplanted kidney tissues of mice were significantly correlated with pathological scores related to acute rejection (all P < 0.05).  Conclusions  Selective deletion of Ndst1 of the donor kidney can alleviate acute rejection after renal transplantation, and the mechanism may be related to the increased 6-O sulfation ratio of disaccharide HS in the transplanted kidney tissues.

     

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