Volume 9 Issue 5
Sep.  2018
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2018  >  9(5): 372-378
Ding Handong, Liao Guiyi, Zhong Jinbiao, et al. Experimental study of protective effect of hypothermic reperfusion on renal ischemia-reperfusion injury in rabbits[J]. ORGAN TRANSPLANTATION, 2018, 9(5): 372-378. doi: 10.3969/j.issn.1674-7445.2018.05.009
Citation: Ding Handong, Liao Guiyi, Zhong Jinbiao, et al. Experimental study of protective effect of hypothermic reperfusion on renal ischemia-reperfusion injury in rabbits[J]. ORGAN TRANSPLANTATION, 2018, 9(5): 372-378. doi: 10.3969/j.issn.1674-7445.2018.05.009
shu

Experimental study of protective effect of hypothermic reperfusion on renal ischemia-reperfusion injury in rabbits

doi: 10.3969/j.issn.1674-7445.2018.05.009

  • Department of Urology, First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University, Anhui Province key Laboratory of Genitourinary Diseases, Hefei 230022, China

More Information
  • Corresponding author: Liao Guiyi, Email: liaoguiyi2@sina.com
  • Received Date: 2018-06-10
    Available Online: 2021-01-19
  • Publish Date: 2018-09-15
    Abstract
  •   Objective  To explore a novel method for establishing rabbit models with renal ischemia-reperfusion injury (IRI) under hypothermic, normothermic and hyperthermic environments and evaluate the effect of the reperfusion under hypothermic environment on renal IRI in rabbits.  Methods  Sixty healthy New Zealand rabbits were randomly and evenly divided into five groups:control group (A group, n=12), sham operation group (B group, n=12), hypothermic reperfusion group (C group, n=12), normothermic reperfusion group (D group, n=12) and hyperthermic reperfusion group (E group, n=12).The serum creatinine (Scr) and blood urea nitrogen (BUN) levels were measured daily within postoperative 7 d.The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in the renal tissues were measured at postoperative 1 d.Hematoxylin-eosin (HE) staining was performed to observe the histopathological changes at postoperative 1 d.Terminal deoxynucleotidyl transferase (TdT)-dUTP nick-end labeling (TUNEL staining) was adopted to detect the cellular apoptosis at postoperative 1 d.  Results  At 1 d after operation, the Scr and BUN levels in C, D and E groups were significantly higher than those in A and B groups (all P < 0.01).Compared with C group, the Scr and BUN levels were more notably increased in D and E groups (both P < 0.05).Within 7 d after surgery, the Scr and BUN levels tended to decline in C, D and E groups.Compared with D and E groups, the Scr and BUN levels were significantly lower in C group (both P < 0.05).Compared with A and B groups, the MDA contents were considerably increased, whereas the SOD activities were significantly decreased in C, D and E groups (all P < 0.01).Compared with C group, the MDA content was more significantly enhanced, whereas the SOD activity was remarkably decreased in D and E groups (both P < 0.01).At postoperative 1 d, the pathological examination of the renal tissues demonstrated that the renal morphology was normal in A and B groups, whereas significant renal damage was observed in D and E groups.Compared with D and E groups, the severity of renal injury was less in C group.TUNEL staining revealed that the quantity of positive renal tubular epithelial cells was significantly increased in D and E groups, and positive cells were also seen in the lumen.The quantity of positive cells in C group was significantly lower than those in D and E groups.  Conclusions  The reperfusion rabbit models under different temperature environments can be established by covering the kidney by ice soil and persistent administration of 37 ℃ and 40 ℃ saline.Hypothermic reperfusion exerts a protective effect on renal IRI.

     

    • FullText(HTML)
  • loading
    • References(18)
  • [1]
    ZUK A, BONVENTRE JV.Acute Kidney Injury[J].Annu Rev Med, 2016, 67:293-307.DOI: 10.1146/annurev-med-050214-013407.
    [2]
    PERICO N, CATTANEO D, SAYEGH MH, et al.Delayed graft function in kidney transplantation[J].Lancet, 2004, 364(9447):1814-1827. doi: 10.1016/S0140-6736(04)17406-0
    [3]
    YANG Y, SONG M, LIU Y, et al.Renoprotective approaches and strategies in acute kidney injury[J].Pharmacol Ther, 2016, 163:58-73.DOI: 10.1016/j.pharmthera.2016.03.015.
    [4]
    NIEMANN CU, FEINER J, SWAIN S, et al.Therapeutic hypothermia in deceased organ donors and kidney-graft function[J].N Engl J Med, 2015, 373(5):405-414.DOI: 10.1056/NEJMoa1501969.
    [5]
    CHENOUNE M, LIDOUREN F, ADAM C, et al.Ultrafast and whole-body cooling with total liquid ventilation induces favorable neurological and cardiac outcomes after cardiac arrest in rabbits[J].Circulation, 2011, 124(8):901-911, 1-7.DOI: 10.1161/CIRCULATIONAHA.111.039388.
    [6]
    林亚平, 刘琴, 陈楚淘, 等.针刺联合亚低温对脑缺血再灌注损伤大鼠脑组织MAPK/ERK通路及凋亡相关因子的影响[J].中南大学学报(医学版), 2017, 42(4):380-388.DOI: 10.11817/j.issn.1672-7347.2017.04.003.

    LIN YP, LIU Q, CHEN CT, et al.Effect of acupuncture combined with hypothermia on MAPK/ERK pathway and apoptosis related factors in rats with cerebral ischemia reperfusion injury[J].J Centr South Univ(Med Sci), 2017, 42(4):380-388.DOI: 10.11817/j.issn.1672-7347.2017.04.003.
    [7]
    FENG JZ, WANG WY, ZENG J, et al.Optimization of brain metabolism using metabolic-targeted therapeutic hypothermia can reduce mortality from traumatic brain injury[J].J Trauma Acute Care Surg, 2017, 83(2):296-304.DOI: 10.1097/TA.0000000000001522.
    [8]
    TISSIER R, COHEN MV, DOWNEY JM.Does mild hypothermia protect against reperfusion injury? the debate continues[J].Basic Res Cardiol, 2011, 106(5):691-695.DOI: 10.1007/s00395-011-0194-8.
    [9]
    GOLDFARB DA.Re:therapeutic hypothermia in deceased organ donors and kidney-graft function[J].J Urol, 2016, 195(5):1549.DOI: 10.1016/j.juro.2016.02.010.
    [10]
    余晓东, 廖波, 邓显忠, 等.一种新型实用的大鼠肾缺血再灌注损伤模型的建立[J].重庆医学, 2011, 40(13):1283-1284, 封3-封4.DOI: 10.3969/j.issn.1671-8348.2011.13.014.

    YU XD, LIAO B, DENG XZ, et al.Model construction of renal ischemia-reperfusion injury in rats[J].Chongqing Med, 2011, 40(13):1283-1284, cover3-4.DOI: 10.3969/j.issn.1671-8348.2011.13.014.
    [11]
    SALVADORI M, ROSSO G, BERTONI E.Update on ischemia-reperfusion injury in kidney transplantation:pathogenesis and treatment[J].World J Transplant, 2015, 5(2):52-67.DOI: 10.5500/wjt.v5.i2.52.
    [12]
    DELBRIDGE MS, SHRESTHA BM, RAFTERY AT, et al.The effect of body temperature in a rat model of renal ischemia-reperfusion injury[J].Transplant Proc, 2007, 39(10):2983-2985. doi: 10.1016/j.transproceed.2007.04.028
    [13]
    蔺艳, 何涛, 毛晓燕, 等.还原型谷胱甘肽对肾急性缺血再灌注性损伤的作用[J].实用医学杂志, 2016, 32(8):1233-1236.DOI:10.3969/j.issn.1006-5725.2016. 08.010.

    LIN Y, HE T, MAO XY, et al.Protective effect of reduced glutathione on kidney against acute ischemia-reperfusion injury[J].J Pract Med, 2016, 32(8):1233-1236.DOI: 10.3969/j.issn.1006-5725.2016.08.010.
    [14]
    AKHTAR MZ, SUTHERLAND AI, HUANG H, et al.The role of hypoxia-inducible factors in organ donation and transplantation:the current perspective and future opportunities[J].Am J Transplant, 2014, 14(7):1481-1487.DOI: 10.1111/ajt.12737.
    [15]
    AYODELE M, KOCH S.Ischemic preconditioning in the intensive care unit[J].Curr Treat Options Neurol, 2017, 19(6):24.DOI: 10.1007/s11940-017-0457-2.
    [16]
    艾娜, 谢席胜, 樊均明, 等.大鼠肾缺血再灌注损伤模型改良手术方式结果与评价[J].中国中西医结合肾病杂志, 2013, 14(2):104-106, 后插2.DOI: 10.3969/j.issn.1009-587X.2013.02.004.

    AI N, XIE XS, FAN JM, et al.The Improved model of the operation mode of renal ischemia reperfusion injury in rats and evaluation[J].Chin J Integr Trad West Nephrol, 2013, 14(2):104-106, back insertion 2.DOI: 10.3969/j.issn.1009-587X.2013.02.004.
    [17]
    韦星, 蔡明, 石炳毅, 等.新型肾脏冷缺血再灌注损伤动物模型的稳定性研究[J/CD].中华临床医师杂志(电子版), 2012, 6(5): 1207-1210.DOI: 10.3877/cma.j.issn.1674-0785.2012.05.030.

    WEI X, CAI M, SHI BY, et al.Stability of a new model of kidney cold ischemia reperfusion injury[J/CD].Chin J Clin (Electr Edit), 2012, 6(5): 1207-1210.DOI: 10.3877/cma.j.issn.1674-0785.2012.05.030.
    [18]
    PALLER MS, HOIDAL JR, FERRIS TF.Oxygen free radicals in ischemic acute renal failure in the rat[J].J Clin Invest, 1984, 74(4):1156-1164. doi: 10.1172/JCI111524
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (78) PDF downloads(11) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return