Effect of tacrolimus on short chain fatty acids in rats

Huang Zhongli; Jiang Yamei; Song Turun; Wang Xianding; Fan Yu; Liu Jinpeng; Wang Zhiling; Wang Li; Lin Tao

doi:10.3969/j.issn.1674-7445.2018.04.012

Volume 9 Issue 4

Jul. 2018

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Huang Zhongli, Jiang Yamei, Song Turun, et al. Effect of tacrolimus on short chain fatty acids in rats[J]. ORGAN TRANSPLANTATION, 2018, 9(4): 311-315. doi: 10.3969/j.issn.1674-7445.2018.04.012

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Huang Zhongli, Jiang Yamei, Song Turun, et al. Effect of tacrolimus on short chain fatty acids in rats[J]. ORGAN TRANSPLANTATION, 2018, 9(4): 311-315. doi: 10.3969/j.issn.1674-7445.2018.04.012

Citation:

Huang Zhongli, Jiang Yamei, Song Turun, et al. Effect of tacrolimus on short chain fatty acids in rats[J]. ORGAN TRANSPLANTATION, 2018, 9(4): 311-315. doi: 10.3969/j.issn.1674-7445.2018.04.012

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Effect of tacrolimus on short chain fatty acids in rats

doi: 10.3969/j.issn.1674-7445.2018.04.012

Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China

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Corresponding author: Lin Tao, Email:kidney5@163.com
Received Date: 2018-04-26
Available Online: 2021-01-19
Publish Date: 2018-07-15

Abstract

Abstract

Objective To investigate the changes of short chain fatty acids (SCFA) induced by tacrolimus (FK506) in rats and evaluate its effect on blood glucose levels. Methods Ten SD rats were divided into the FK506 group and control group (n=5 in each group). In the FK506 group, the rats were received a subcutaneous injection of FK506 (3 mg/kg) +sunflower oil solution containing 10% ethanol daily for consecutive 4 weeks. In the control group, the rats were received a subcutaneous injection of an equivalent amount of sunflower oil solution containing 10% ethanol for consecutive 4 weeks. During the drug injection period, the body mass of rats was measured every week in two groups. After the drug injection period, blood glucose level, SCFA content in the blood and feces samples were measured in two groups. Results Compared with the control group, the relative body mass of rats in the FK506 group was significantly lower at the 2^nd, 3^rd and 4^th weeks (all P < 0.01). Compared with the control group, the blood glucose levels of rats in the FK506 group were significantly increased at 0, 30, and 60 min after giving glucose (P < 0.01-0.05). Compared with the control group, the contents of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid and valeric acid in the feces sample were significantly lower in the FK506 group (P < 0.01-0.05). Conclusions FK506 can upregulate the blood glucose level in rats, which is probably induced by the decrease of SCFA content in rat feces.
- Rat,
- Tacrolimus,
- Blood glucose,
- Short chain fatty acids,
- Body mass,
- Post-transplant diabetes mellitus,
- Gut flora

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Effect of tacrolimus on short chain fatty acids in rats

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