Volume 9 Issue 3
May  2018
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2018  >  9(3): 188-193
Li Rui, Dong Hongli, Liu Baolin. Study of isolation efficiency of new islet isolation solution for mouse islets[J]. ORGAN TRANSPLANTATION, 2018, 9(3): 188-193. doi: 10.3969/j.issn.1674-7445.2018.03.004
Citation: Li Rui, Dong Hongli, Liu Baolin. Study of isolation efficiency of new islet isolation solution for mouse islets[J]. ORGAN TRANSPLANTATION, 2018, 9(3): 188-193. doi: 10.3969/j.issn.1674-7445.2018.03.004
shu

Study of isolation efficiency of new islet isolation solution for mouse islets

doi: 10.3969/j.issn.1674-7445.2018.03.004

  • Department of Hepatobiliary Surgery, the People's Hospital of China Medical University, Shenyang 110016, China

More Information
  • Corresponding author: Liu Baolin, Email: liubl55@hotmail.com
  • Received Date: 2018-01-21
    Available Online: 2021-01-19
  • Publish Date: 2018-05-15
    Abstract
  •   Objective  To investigate the isolation and protective effect of a new islet purification solution (IPS)- Optiprep solution on the islet in mouse models.  Methods  The digested pancreatic islets were divided into the IPS andUW groups according to the islet volume. The pancreatic islets were isolated by the continuous gradient density centrifugation using IPS-Optiprep or UW-Optiprep solutions. The purification efficiency and isolated islet activity of purification solution were compared between two groups. The diabetic mouse models were successfully induced and randomly assigned into three groups. In the experimental group (n=10), the mice received pancreatic islet transplantation using islets isolated and purified by the IPS-Optiprep solution. In the control group (n=10), the mice underwent pancreatic islet transplantation using islets isolated and purified by the UW-Optiprep solution. In the sham surgery group (n=5), the mice merely underwent surgery without pancreatic islet transplantation. Postoperative blood glucose levels were detected and compared among three groups. The blood glucose levels of intraperitoneal glucose tolerance test at postoperative 21 d were statistically compared between the experimental and control groups. The cost of the preparation of two isolation solutions was also compared.  Results  Compared with the UW group, the islet equivalent (IEQ), islet purity, recovery rate and islet integrity were significantly higher in the IPS group. Islet morphological observation revealed that the islet membrane was complete and the islet diameter in the IPS group was considerably larger than that in the UW group. The activity of purified islets in the UW group was significantly higher than that in the IPS group [(88±5)% vs. (84±3)%, P < 0.01]. Compared with the UW-Optiprep solution, identical in vivo islet function was obtained in the IPS-Optiprep solution. The cost of IPS-Optiprep solution was significantly less than that of the UW-Optiprep solution.  Conclusions  The new IPS-Optiprep solution yields higher islet isolation efficiency, purification, integrity and recovery rate and significantly reduces the purification cost compared with the UW-Optiprep solution. Nevertheless, IPS-Optiprep solution exerts a less protective effect on the activity of islet cells, which is probably correlated with the high islet integrity and the endotoxin in the IPS-Optiprep solution.

     

    • FullText(HTML)
  • loading
    • References(20)
  • [1]
    SHAPIRO AM, RICORDI C, HERING BJ, et al. International trial of the Edmonton protocol for islet transplantation[J]. N Engl J Med, 2006, 355(13):1318- 1330. DOI: 10.1056/NEJMoa061267.
    [2]
    王维.异种胰岛移植研究现状和走向临床的关键科学问题[J].器官移植, 2017, 8(6): 413-416. DOI: 10.3969/j.issn.1674-7445.2017.06.001.

    WANG W. Research status of islet xenotransplantation and key scientific problems toward clinic[J]. Organ Transplant, 2017, 8(6): 413-416. DOI: 10.3969/j.issn.1674-7445.2017.06.001.j.issn.1674-7445.2017.06.001.
    [3]
    JAMIOLKOWSKI RM, GUO LY, LI YR, et al. Islet transplantation in type I diabetes mellitus[J]. Yale J Biol Med, 2012, 85(1): 37-43. http://cn.bing.com/academic/profile?id=90b8bf7218e873a2879d420e27a77f0a&encoded=0&v=paper_preview&mkt=zh-cn
    [4]
    MERANI S, SHAPIRO AM. Current status of pancreatic islet transplantation[J]. Clin Sci (Lond), 2006, 110(6): 611-625. DOI: 10.1042/CS20050342.
    [5]
    KIN T. Islet isolation for clinical transplantation[J]. Adv Exp Med Biol, 2010, 654: 683-710. DOI: 10.1007/978- 90-481-3271-3_30.
    [6]
    NOGUCHI H, NAZIRUDDIN B, SHIMODA M, et al. Evaluation of osmolality of density gradient for human islet purification[J]. Cell Transplant, 2012, 21(2/3): 493- 500. DOI: 10.3727/096368911X605402.
    [7]
    MITA A, RICORDI C, MESSINGER S, et al. Antiproinflammatory effects of iodixanol (OptiPrep)- based density gradient purification on human islet preparations[J]. Cell Transplant, 2010, 19(12): 1537- 1546. DOI: 10.3727/096368910X516600.
    [8]
    LIU B, HAO J, PAN Y, et al. Increasing donor chimerism and inducing tolerance to islet allografts by post-transplant donor lymphocyte infusion[J]. Am J Transplant, 2006, 6(5 Pt 1): 933-946. DOI: 10.1111/j.1600-6143. 2006.01283.x.
    [9]
    吴瑛, 张勇, 姚合斌.高脂联合小剂量链脲佐菌素建立实验性2型糖尿病大鼠模型[J].转化医学杂志, 2017, 6(6): 355-357. DOI:10.3969/j.issn.2095-3097.2017.06.009.

    WU Y, ZHANG Y, YAO HB. Study of streptozotocin and high-fat diet inducing type 2 diabetes in a rat model[J]. Translat Med J, 2017, 6(6): 355-357. DOI: 10.3969/j.issn.2095-3097.2017.06.009.j.issn.2095-3097.2017.06.009.
    [10]
    MCCALL MD, MACIVER AH, PAWLICK R, et al. Histopaque provides optimal mouse islet purification kinetics: comparison study with ficoll, iodixanol and dextran[J]. Islets, 2011, 3(4): 144-149. doi: 10.4161/isl.3.4.15729
    [11]
    DELLÊ H, SAITO MH, YOSHIMOTO PM, et al. The use of iodixanol for the purification of rat pancreatic islets[J]. Transplant Proc, 2007, 39(2): 467-469. DOI: 10.1016/j.transproceed.2007.01.039.
    [12]
    RIDGWAY D, MANAS D, SHAW J, et al. Preservation of the donor pancreas for whole pancreas and islet transplantation[J]. Clin Transplant, 2010, 24(1): 1-19. DOI: 10.1111/j.1399-0012.2009.01151.x.
    [13]
    BAERTSCHIGER RM, BERNEY T, MOREL P. Organ preservation in pancreas and islet transplantation[J]. Curr Opin Organ Transplant, 2008, 13(1): 59-66. DOI: 10.1097/MOT.0b013e3282f44a63.
    [14]
    HUANG GC, ZHAO M, JONES P, et al. The development of new density gradient media for purifying human islets and islet-quality assessments[J]. Transplantation, 2004, 77(1): 143-145. DOI: 10.1097/01.TP.0000100401.62912.B2.
    [15]
    LAU J, HENRIKSNÄS J, SVENSSON J, et al. Oxygenation of islets and its role in transplantation[J]. Curr Opin Organ Transplant, 2009, 14(6): 688-693. DOI: 10.1097/MOT.0b013e32833239ff.
    [16]
    UMEADI C, KANDEEL F, AL-ABDULLAH IH. Ulinastatin is a novel protease inhibitor and neutral protease activator[J]. Transplant Proc, 2008, 40(2): 387- 389. DOI: 10.1016/j.transproceed.2008.01.034.
    [17]
    NOGUCHI H, UEDA M, NAKAI Y, et al. Modified twolayer preservation method (M-Kyoto/PFC) improves islet yields in islet isolation[J]. Am J Transplant, 2006, 6(3): 496-504. DOI: 10.1111/j.1600-6143.2006.01223.x.
    [18]
    NOGUCHI H, UEDA M, HAYASHI S, et al. Comparison of trypsin inhibitors in preservation solution for islet isolation[J]. Cell Transplant, 2009, 18(5): 541-547. http://cn.bing.com/academic/profile?id=2237d5f01fed996142d2686b4026b62b&encoded=0&v=paper_preview&mkt=zh-cn
    [19]
    NOGUCHI H. Pancreas procurement and preservation for islet transplantation: personal considerations[J]. J Transplant, 2011: 783168. DOI: 10.1155/2011/783168.
    [20]
    BRISSOVA M, POWERS AC. Revascularization of transplanted islets: can it be improved?[J]. Diabetes, 2008, 57(9): 2269-2271. DOI: 10.2337/db08-0814.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (116) PDF downloads(6) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return