Volume 8 Issue 3
May  2017
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Bao Jie, Zhang Juan, Wang Jing. MicroRNA-101 inhibits epithelial-mesenchymal transition in human liver carcinoma MHCC97H cells via USP22[J]. ORGAN TRANSPLANTATION, 2017, 8(3): 209-214. doi: 10.3969/j.issn.1674-7445.2017.03.007
Citation: Bao Jie, Zhang Juan, Wang Jing. MicroRNA-101 inhibits epithelial-mesenchymal transition in human liver carcinoma MHCC97H cells via USP22[J]. ORGAN TRANSPLANTATION, 2017, 8(3): 209-214. doi: 10.3969/j.issn.1674-7445.2017.03.007
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MicroRNA-101 inhibits epithelial-mesenchymal transition in human liver carcinoma MHCC97H cells via USP22

doi: 10.3969/j.issn.1674-7445.2017.03.007

  • Department of Internal Medicine, South China Normal University Hospital, Guangzhou 510630, China

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  • Corresponding author: Bao Jie, Email: 12706446@qq.com
  • Received Date: 2017-02-23
    Available Online: 2021-01-19
  • Publish Date: 2017-05-15
    Abstract
  •   Objective   To investigate the effect and underlying mechanism of micro ribonucleic acid (miR)-101 on the epithelial-mesenchymal transition in human hepatocellular carcinoma MHCC97H cells.   Methods   MHCC97H cell lines were transfected with miR-101 mimics (M101 group) and negative control mimic (NCM group), and the cell lines without transfection were used as the control group. The expression levels of miR-101 in cells of 3 groups were quantitatively measured using reverse transcription polymerase chain reaction (RT-PCR). Transwell assay was performed to evaluate the cell migration and invasion ability of 3 groups. The expression levels of vimentin, -catenin, E-cadherin and USP22 proteins in cells of 3 groups were measured by Western blot. The relationship between miR-101 and USP22 was analyzed by dual-luciferase assay.   Results   In the M101 group, the expression level of miR-101 was significantly up-regulated, which was approximately 761 times of that in the control group (P < 0.05). In the M101 group, the quantity of migrating cells was 15.7±1.6, significantly lower than 94.1±1.8 in the control group (P < 0.05). In the M101 group, the quantity of invasive cells was 9.1±0.4, significantly lower compared with 51.6±0.9 in the control group (P < 0.05). In the M101 group, the expression levels of vimentin and USP22 protein were significantly down-regulated, whereas the expression levels of -catenin and E-cadherin protein were significantly up-regulated. Dual-luciferase assay revealed that USP22 was the target gene of the downstream miR-101 signaling pathway.   Conclusion   miR-101 regulates the expression of epithelial-mesenchymal transition-related proteins and suppresses the epithelial-mesenchymal transition of hepatocellular carcinoma MHCC97H cells probably through down-regulating the expression level of USP22.

     

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