Volume 8 Issue 2
Mar.  2017
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2017  >  8(2): 154-160
Cao Yi, Lyu Lizhi, Jiang Yi, et al. Relationship between CNTN-1 and VEGF-C expression levels and recurrence, metastasis and prognosis of hepatocellular carcinoma patients after liver transplantation[J]. ORGAN TRANSPLANTATION, 2017, 8(2): 154-160. doi: 10.3969/j.issn.1674-7445.2017.02.012
Citation: Cao Yi, Lyu Lizhi, Jiang Yi, et al. Relationship between CNTN-1 and VEGF-C expression levels and recurrence, metastasis and prognosis of hepatocellular carcinoma patients after liver transplantation[J]. ORGAN TRANSPLANTATION, 2017, 8(2): 154-160. doi: 10.3969/j.issn.1674-7445.2017.02.012
shu

Relationship between CNTN-1 and VEGF-C expression levels and recurrence, metastasis and prognosis of hepatocellular carcinoma patients after liver transplantation

doi: 10.3969/j.issn.1674-7445.2017.02.012

  • Clinical Medical School, Fuzhou General Hospital of Nanjing Military Area Command, Fujian Medical University, Fuzhou 350025, China

More Information
  • Corresponding author: Lyu Lizhi, Email:fzzyllz@qq.com
  • Received Date: 2016-11-20
    Available Online: 2021-01-19
  • Publish Date: 2017-03-15
    Abstract
  •   Objective  To investigate the relationship between contactin (CNTN)-1 and vascular endothelial growth factor (VEGF)-C expression levels and the recurrence, metastasis and prognosis of hepatocellular carcinoma (HCC) patients after liver transplantation.  Methods  Clinical data and pathological specimen of 105 patients diagnosed with primary HCC undergoing orthotopic liver transplantation were collected. The expression levels of CNTN-1 and VEGF-C in the cancerous and para-cancerous liver tissues were quantitatively measured by immunohistochemical staining. The relationship between the CNTN-1 and VEGF-C expression levels and clinicopathological characteristics, postoperative recurrence, metastasis and prognosis was statistically analyzed.  Results  The high expression rate of CNTN-1 and VEGF-C in liver cancerous tissues was 46.7% and 39.0%, significantly higher compared with 11.4% and 19.0% in the para-cancerous tissues (both P < 0.05). Spearman correlation analysis revealed that the expression level of CNTN-1 was positively correlated with that of VEGF-C (P < 0.005). χ2 test demonstrated that the expression of CNTN-1 protein was positively correlated with the level of alpha fetoprotein (AFP) (P=0.017), tumor, node, metastasis (TNM) staging (all P < 0.001), and negatively correlated with the degree of differentiation (P < 0.001). High expression of VEGF-C was positively correlated with TNM staging (P < 0.001). Cox multivariate analysis revealed that overall survival rate was significantly correlated with gender, AFP, degree of tumor differentiation, microvascular invasion, tumor diameter and high expression of CNTN-1 (P < 0.05-0.001). The recurrence-free survival was correlated with TNM staging, envelope integrity and high CNTN-1 expression (P < 0.05-0.001). Kaplan-Meier survival analysis revealed that postoperative overall survival curve and recurrence-free survival curve significantly differed between patients with high and low expression levels of CNTN-1 (both P < 0.01). Recurrence-free survival curve significantly differed between patients with high and low expression levels of VEGF-C (P=0.005).  Conclusions  CNTN-1 protein is highly expressed in HCC tissues and correlated with the expression of VEGF-C. It is associated with postoperative recurrence and metastasis of HCC after liver transplantation and affects the clinical prognosis of patients.

     

    • FullText(HTML)
  • loading
    • References(17)
  • [1]
    Kew MC. Hepatocellular carcinoma: epidemiology and risk factors[J]. J Hepatocell Carcinoma, 2014, 1:115-125. DOI: 10.2147/JHC.S44381.
    [2]
    Liu P, Chen S, Wu W, et al. Contactin-1 (CNTN-1) overexpression is correlated with advanced clinical stage and lymph node metastasis in oesophageal squamous cell carcinomas.[J]. Jpn J Clin Oncol, 2012, 42(7):612-618. DOI: 10.1093/jjco/hys066.
    [3]
    Shi K, Xu D, Yang C, et al. Contactin 1 as a potential biomarker promotes cell proliferation and invasion in thyroid cancer[J]. Int J Clin Exp Pathol, 2015, 8(10): 12473-12481.
    [4]
    Yu JW, Wu SH, Lu RQ, et al. Expression and significances of contactin-1 in human gastric cancer.[J]. Gastroenterol Res Pract, 2013:210205. DOI: 10.1155/2013/210205.
    [5]
    Liu P, Zhou J, Zhu H, et al. VEGF-C promotes the development of esophageal cancer via regulating CNTN-1 expression[J]. Cytokine, 2011, 55(1):8-17. DOI: 10.1016/j.cyto.2011.03.008.
    [6]
    Al-Shareef H, Hiraoka SI, Tanaka N, et al. Use of NRP1, a novel biomarker, along with VEGF-C, VEGFR-3, CCR7 and SEMA3E, to predict lymph node metastasis in squamous cell carcinoma of the tongue[J]. Oncol Rep, 2016, 36(5):2444-2454. DOI: 10.3892/or.2016.5116.
    [7]
    Su CM, Su YH, Chiu CF, et al. Vascular endothelial growth factor-C upregulates cortactin and promotes metastasis of esophageal squamous cell carcinoma[J]. Ann Surg Oncol, 2014, 21 (Suppl 4):S767-S775. DOI: 10.1245/s10434-014-4009-7.
    [8]
    Liang B, Li Y. Prognostic significance of VEGF-C expression in patients with breast cancer: a Meta-analysis[J]. Iran J Public Health, 2014, 43(2):128-135. https://www.cabdirect.org/cabdirect/abstract/20143105821
    [9]
    Wang W, Li GY, Zhu JY, et al. Overexpression of AGGF1 is correlated with angiogenesis and poor prognosis of hepatocellular carcinoma[J]. Med Oncol, 2015, 32(4):131. DOI: 10.1007/s12032-015-0574-2.
    [10]
    Andreou A, Bahra M, Schmelzle M, et al. Predictive factors for extrahepatic recurrence of hepatocellular carcinoma following liver transplantation[J]. Clin Transplant, 2016, 30(7):819-827. DOI: 10.1111/ctr.12755.
    [11]
    Wu HM, Cao W, Ye D, et al. Contactin 1 (CNTN1) expression associates with regional lymph node metastasis, and is a novel predictor of prognosis in patients with oral squamous cell carcinoma[J]. Mol Med Rep, 2012, 6(2):265-270. DOI: 10.3892/mmr.2012.910.
    [12]
    Chen DH, Yu JW, Wu JG, et al. Significances of contactin-1 expression in human gastric cancer and knockdown of contactin-1 expression inhibits invasion and metastasis of MKN45 gastric cancer cells[J]. J Cancer Res Clin Oncol, 2015, 141(12):2109-2120. DOI: 10.1007/s00432-015-1973-7.
    [13]
    Li GY, Huang M, Pan TT, et al. Expression and prognostic significance of contactin 1 in human hepatocellular carcinoma[J]. Onco Targets Ther, 2016, 9:387-394. DOI: 10.2147/OTT.S97367.
    [14]
    Wang XL, Fang JP, Tang RY, et al. Different significance between intratumoral and peritumoral lymphatic vessel density in gastric cancer: a retrospective study of 123 cases[J]. BMC Cancer, 2010, 10:299. DOI: 10.1186/1471-2407-10-299.
    [15]
    Zong S, Li H, Shi Q, et al. Prognostic significance of VEGF-C immunohistochemical expression in colorectal cancer: a meta-analysis[J]. Clin Chim Acta, 2016, 458:106-114. DOI: 10.1016/j.cca.2016.04.037.
    [16]
    Chen H, Guan R, Lei Y, et al. Lymphangiogenesis in gastric cancer regulated through Akt/mTOR-VEGF-C/VEGF-D axis[J]. BMC Cancer, 2015, 15: 103. DOI: 10.1186/s12885-015-1109-0.
    [17]
    He M, Cheng Y, Li W, et al. Vascular endothelial growth factor C promotes cervical cancer metastasis via up-regulation and activation of RhoA/ROCK-2/moesin cascade[J]. BMC Cancer, 2010, 10:170. DOI: 10.1186/1471-2407-10-170.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(2)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (157) PDF downloads(5) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return