Volume 8 Issue 1
Jan.  2017
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2017  >  8(1): 27-33
Cao Weiwei, Li Xiao, Xu Jiru. Study of antibacterial effect of mononuclear cells in liver lavage solution[J]. ORGAN TRANSPLANTATION, 2017, 8(1): 27-33. doi: 10.3969/j.issn.1674-7445.2017.01.006
Citation: Cao Weiwei, Li Xiao, Xu Jiru. Study of antibacterial effect of mononuclear cells in liver lavage solution[J]. ORGAN TRANSPLANTATION, 2017, 8(1): 27-33. doi: 10.3969/j.issn.1674-7445.2017.01.006
shu

Study of antibacterial effect of mononuclear cells in liver lavage solution

doi: 10.3969/j.issn.1674-7445.2017.01.006

  • Department of Immunology and Pathogenic Biology, Medical College of Xi'an Jiaotong University, Xi'an 710061, China

More Information
  • Corresponding author: Xu Jiru, Email:xujiru@mail.xjtu.edu.cn
  • Received Date: 2016-10-18
    Available Online: 2021-01-19
  • Publish Date: 2017-01-15
    Abstract
  •   Objective  To evaluate the antibacterial effect of mononuclear cells (MCs) in the liver lavage solution.  Methods  For in vitro experiment, MCs were collected from the liver lavage solution of SD rats and divided into the supplement of interleukin (IL)-15 and non-supplement groups. The MCs were co-cultured with Pseudomonas aeruginosa (P. aeruginosa) for 4 h and then the supernatant was collected and MCs were lysed. The bacterial load in the lysate was detected after LB plate culture. The levels of interferon (IFN)-γ and tumor necrosis factor (TNF)-α in the supernatant were measured by enzyme-linked immune absorbent assay (ELISA). For in vivo experiment, 40 SD rats were administered via tracheal injection of P. aeruginosa solution at a dose of 1×109 CFU/mL and randomly divided into four groups (n=10). In the control group, physiological saline was given via gavage. In the immunosuppression group, tacrolimus (FK506) was delivered via gavage. In the MC group, MCs at a dose of 1.0×108 was given via intravenous injection after use of FK506. In the IL-15 pretreated-MC group, IL-15 pretreated-MCs at a dose of 1.0×108 were administered via intravenous injection after application of FK506. The lavage solution of pulmonary alveolus and the rat lung tissue were collected. The bacterial load was detected after LB plate culture. The expression of IFN-γ and TNF-α in the pulmonary alveolus and lung tissue were measured by ELISA and Western blot.  Results  Compared with MCs alone, IL-15 pretreated-MCs exhibited significantly higher antibacterial capability in vitro. The CFU was 35% of untreated MCs. The synthesis and release capabilities of IFN-γ and TNF-α were significantly enhanced. Compared with the control group, the quantity of immune cells in the lung tissue was decreased and the bacterial load in the lung tissue and the lavage solution of pulmonary alveolus was significantly increased, whereas the expression levels of IFN-γ and TNF-α tended to decline in the immunosuppression group. Administration of IL-15 pretreated-MCs significantly enhanced the quantity of immune cells in the lung tissue, decreased the bacterial load and increased the secretion of IFN-γ and TNF-α.  Conclusions  MCs in the liver lavage solution exhibit favorable antibacterial activity. Under immunosuppression condition, the defense capability of the host against the opportunistic pathogenic bacteria is significantly enhanced.

     

    • FullText(HTML)
  • loading
    • References(26)
  • [1]
    Kawecki D, Chmura A, Pacholczyk M, et al. Bacterial infections in the early period after liver transplantation: etiological agents and their susceptibility [J]. Med Sci Monit, 2009, 15(12): CR628-CR637.
    [2]
    Sullivan T, Weinberg A, Rana M, et al. The epidemiology and clinical features of clostridium difficile infection in liver transplant recipients [J]. Transplantation, 2016, 100(9): 1939-1943. DOI: 10.1097/TP.0000000000001309.
    [3]
    Kim SI, Kim YJ, Jun YH, et al. Epidemiology and risk factors for bacteremia in 144 consecutive living-donor liver transplant recipients [J]. Yonsei Med J, 2009, 50(1): 112-121. DOI: 10.3349/ymj.2009.50.1.112.
    [4]
    Iida T, Kaido T, Yagi S, et al. Posttransplant bacteremia in adult living donor liver transplant recipients [J]. Liver Transpl, 2010, 16(12): 1379-1385. DOI: 10.1002/lt.22165.
    [5]
    Siniscalchi A, Aurini L, Benini B, et al. Ventilator associated pneumonia following liver transplantation: etiology, risk factors and outcome [J]. World J Transplant, 2016, 6(2): 389-395. DOI: 10.5500/wjt.v6.i2.389.
    [6]
    Smith CT, Katz MG, Foley D, et al. Incidence and risk factors of incisional hernia formation following abdominal organ transplantation [J]. Surg Endosc, 2015, 29(2): 398-404. DOI: 10.1007/s00464-014-3682-8.
    [7]
    陈肖, 张红.肝移植围手术期预防感染的护理对策研究[J].河北医学, 2014, 21(5): 846-849. DOI: 10.3969 /j.issn.1006-6233.2015.05.050.

    Chen X, Zhang H. Nursing strategies for perioperative prophylaxis of infection in liver transplantation [J]. Hebei Med, 2014, 21(5): 846-849. 10.3969 /j.issn.1006-6233.2015.05.050. doi: 10.3969/j.issn.1006-6233.2015.05.050
    [8]
    刘建明, 杨永洁, 刘大钺, 等.肝移植术后医院感染流行特征分析[J].中华医院感染学杂志, 2011, 2l (19): 4022-4024. http://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY201119030.htm

    Liu JM, Yang YJ, Liu DY, et al. Epidemiological analysis of nosocomial infections after liver transplantation [J]. Chin J Nosocomiol, 2011, 2l (19): 4022-4024. http://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY201119030.htm
    [9]
    Fernández-Ruiz M, Silva JT, López-Medrano F, et al. Post-transplant monitoring of NK cell counts as a simple approach to predict the occurrence of opportunistic infection in liver transplant recipients [J]. Transpl Infect Dis, 2016, 18(4): 552-565. DOI: 10.1111/tid.12564.
    [10]
    Ohira M, Ishiyama K, Tanaka Y, et al. Adoptive immunotherapy with liver allograft-derived lymphocytes induce anti-HCV activity after liver transplantation in humans and humanized mice [J]. J Clin Invest, 2009, 119(11): 3226-3235. DOI: 10.1172/JCI38374.
    [11]
    Cheent K, Khakoo SI. Natural killer cells and hepatitis C: action and reaction [J]. Gut, 2011, 60(2): 268-278. DOI: 10.1136/gut.2010.212555.
    [12]
    Ohira M, Nishida S, Matsuura T, et al. Comparative analysis of T-cell depletion method for clinical immunotherapy-anti-hepatitis c effects of natural killer cells via interferon-γ production [J]. Transplant Proc, 2013, 45(5): 2045-2050. DOI: 10.1016/j.transproceed.2013.01.046.
    [13]
    Chen H, Li F, Zhan Y, et al. Circulating cytokine portraits can differentiate between allograft rejection and pulmonary infection in cardiac transplant rats [J]. Interact Cardiovasc Thorac Surg, 2016, 23(1): 118-124. DOI: 10.1093/icvts/ivw051.
    [14]
    Howell J, Sawhney R, Testro A, et al. Cyclosporine and tacrolimus have inhibitory effects on toll-like receptor signaling after liver transplantation [J]. Liver Transpl, 2013, 19(10): 1099-1107. DOI: 10.1002/lt.23712.
    [15]
    Ishiyama K, Ohdan H, Ohira M, et al. Difference in cytotoxicity against hepatocellular carcinoma between liver and periphery natural killer cells in humans [J]. Hepatology, 2006, 43(2): 362-372. doi: 10.1002/(ISSN)1527-3350
    [16]
    Sganga G, Spanu T, Bianco G, et al. Bacterial bloodstream infections in liver transplantation: etiologic agents and antimicrobial susceptibility profiles [J]. Transplant Proc, 2012, 44(7): 1973-1976. DOI: 10.1016/j. transproceed.2012.06.055.
    [17]
    Bodro M, Sabé N, Tubau F, et al. Extensively drug-resistant Pseudomonas aeruginosa bacteremia in solid organ transplant recipients [J]. Transplantation, 2015, 99(3): 616-622. DOI: 10.1097/TP.0000000000000366.
    [18]
    Sato A, Kaido T, Iida T, et al. Bundled strategies against infection after liver transplantation: lessons from multidrug-resistant pseudomonas aeruginosa [J]. Liver Transpl, 2016, 22(4): 436-445. DOI: 10.1002/lt.24407.
    [19]
    Davidson S, Maini MK, Wack A. Disease-promoting effects of type Ⅰ interferons in viral, bacterial, and coinfections [J]. Interferon Cytokine Res, 2015, 35(4): 252-264. DOI: 10.1089/jir.2014.0227.
    [20]
    Clifford V, He Y, Zufferey C, et al. Interferon gamma release assays for monitoring the response to treatment for tuberculosis: a systematic review [J]. Tuberculosis, 2015, 95 (6): 639-650. DOI: 10.1016/j.tube. 2015.07.002.
    [21]
    Morton B, Pennington SH, Gordon SB. Immunomodulatory adjuvant therapy in severe community-acquired pneumonia [J]. Expert Rev Respir Med, 2014, 8(5): 587-596. DOI: 10.1586/17476348. 2014.927736.
    [22]
    Davis CT, Rizzieri D. Immunotherapeutic applications of NK cells [J]. Pharmaceuticals, 2015, 8(2): 250-256. DOI: 10.3390/ph8020250.
    [23]
    Adib-Conquy M, Scott-Algara D, Cavaillon JM, et al. TLR-mediated activation of NK cells and their role in bacterial/viral immune responses in mammals [J]. Immunol Cell Biol, 2014, 92(3): 256-262. DOI: 10.1038/icb.2013.99.
    [24]
    Patidar M, Yadav N, Dalai SK. Interleukin 15: a key cytokine for immunotherapy [J]. Cytokine Growth Factor Rev, 2016, 31: 49-59. DOI: 10.1016/j.cytogfr.2016.06.001.
    [25]
    Marçais A, Cherfils-Vicini J, Viant C, et al. The metabolic checkpoint kinase mTOR is essential for IL-15 signaling during the development and activation of NK cells [J]. Nat Immunol, 2014, 15(8): 749-757. DOI: 10.1038/ni.2936.
    [26]
    Hamon MA, Quintin J. Innate immune memory in mammals [J]. Semin Immunol, 2016, 28(4): 351-358. DOI: 10.1016/j.smim.2016.05.003.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (116) PDF downloads(5) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return