Changes of renal resident dendritic cells during kidney ischemia-reperfusion injury

  Objective  To investigate the changes of renal resident dendritic cells (rDC) during kidney ischemia-reperfusion injury(IRI).

  Methods  C57BL/6J mice models with bilateral renal warm ischemia were established. The kidney tissue was prepared for single cell suspension at 24 h and 48 h after reperfusion. The changes in the percentage of CD45⁺ cells and CD11c⁺rDCs were evaluated by flow cytometry. The renal tissues of mice labeled with green fluorescent protein and diphtheria toxin receptor (CD11c⁺GDTR) were prepared for single cell suspension. The percentage and phenotype of CD11c⁺rDCs were analyzed by flow cytometry. CD11c⁺GDTR mice models with bilateral renal warm ischemia were established. The renal tissue was prepared for single cell suspension at 24 h after reperfusion. CD45⁺ cells was gathered by magnetic-activated cell separation (MACS). The expression levels of co-stimulatory molecules on the rDC surface were analyzed by flow cytometry.

  Results  At 24 h after reperfusion, the percentage of CD45⁺ cells in the kidney of C57BL/6J mice was significantly elevated, and further increased at 48 h after reperfusion. At 24 h after reperfusion, the quantity of CD11c⁺rDCs was equally increased, whereas the percentage of CD11c⁺rDCs in CD45⁺ cells was dramatically declined and restored at 48 h after reperfusion, slightly higher compared with that in the sham group. In healthy CD11c⁺GDTR mice, the percentage of CD45⁺ cells in the kidney was lower than 1%, consisting of approximately 40% of CD11c⁺rDCs, which mainly presented as CD11b^intF4/80^-MHCⅡ⁺. At 24 h after reperfusion, the percentage of CD11c⁺F4/80^- subset rDC surface co-stimulatory molecules was significantly enhanced, such as CD40, CD80 and CD86.

  Conclusions  Following warm IRI, the percentage and quantity of rDCs, and the expression level of rDC surface co-stimulatory molecule are significantly increased, prompting that renal rDC infiltration is increased and phenotype becomes matured.