Volume 7 Issue 3
May  2016
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Article Navigation >  ORGAN TRANSPLANTATION  > 2016  >  7(3): 191-195
Ma Xihui, Huang Haiyan, Gao Yu, et al. Establishment of cytometric bead assay combined detection on Cys C and NGAL and its application in CMV-infected patients after renal transplantation[J]. ORGAN TRANSPLANTATION, 2016, 7(3): 191-195. doi: 10.3969/j.issn.1674-7445.2016.03.006
Citation: Ma Xihui, Huang Haiyan, Gao Yu, et al. Establishment of cytometric bead assay combined detection on Cys C and NGAL and its application in CMV-infected patients after renal transplantation[J]. ORGAN TRANSPLANTATION, 2016, 7(3): 191-195. doi: 10.3969/j.issn.1674-7445.2016.03.006
shu

Establishment of cytometric bead assay combined detection on Cys C and NGAL and its application in CMV-infected patients after renal transplantation

doi: 10.3969/j.issn.1674-7445.2016.03.006

  • Graduate Faculty of Academy of Military Medical Sciences, Beijing 100850, China

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    Abstract
  •   Objective  To establish the method of cytometric bead assay in combined detection of cystatin C (Cys C) and neutrophil gelatinase-associated lipocalin (NGAL) and to preliminarily investigate the changes in the serum levels of Cys C and NGAL in cytomegalovirus (CMV)-infected patients after renal transplantation.   Methods  The experimental method was established as following. The effect and stability of microsphere capsulation were evaluated. The detection range of Cys C and NGAL concentration was improved. The concentrations of biotinylated antibody and PE-streptavidin were quantitatively measured. The correlation coefficient of the standard curve was calculated. The serum levels of Cys C and NGAL in the control (n=10, healthy person), negative CMV (n=10, CMV-pp65 negative patients after renal transplantation) and positive CMV groups (n=20, CMV-pp65 positive patients after renal transplantation) were detected using this method.   Results  The method of cytometric bead assay in combined detection of Cys C and NGAL was established successfully. In the control, negative and positive CMV groups, the serum levels of NGAL were 8 759(1 465) pg/ml, 10 472(856)pg/ml and 12 817(4 533)pg/ml with statistical significance (all P < 0.001). No statistical significance was noted in terms of Cys C concentration among three groups (all P > 0.05).  Conclusions  Combined application of cytometric bead assay detects the standard curve of Cys C and NGAL with high relevance and reproducibility. Preliminary results demonstrates that NGAL concentration is significantly elevated in CMV-infected patients following renal transplantation, hinting that CMV active infection probably leads to acute renal tubular injury.

     

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  • [1]
    Moncunill G, Campo JJ, Dobaño C. Quantification of multiple cytokines and chemokines using cytometric bead arrays[J].Methods Mol Biol, 2014, 1172:65-86. doi: 10.1007/978-1-4939-0928-5
    [2]
    Haase-Fielitz A, Haase M, Devarajan P. Neutrophil gelatinase-associated lipocalin as a biomarker of acute kidney injury:a critical evaluation of current status[J]. Ann Clin Biochem, 2014, 51(3): 335-351. doi: 10.1177/0004563214521795
    [3]
    肖漓, 石炳毅, 黄海燕, 等.肾移植受者外周血巨细胞病毒特异性CTL的比例及其意义探讨[J].中华医学杂志, 2014, 94(42):3289-3292. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-IGMD201410001A0P.htm

    Xiao L, Shi BY, Huang HY, et al. Study on the proportion and significance of cytomegalovirus specific CTL in peripheral blood of renal transplant recipients[J]. Natl Med J Chin, 2014, 94(42):3289-3292. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-IGMD201410001A0P.htm
    [4]
    Wacker-Gußmann A, Bühren K, Schultheiss C, et al. Prediction of contrast-induced nephropathy in patients with serum creatinine levels in the upper normal range by cystatin C: a prospective study in 374 patients [J]. AJR Am J Roentgenol, 2014, 202(2): 452-458. doi: 10.2214/AJR.13.10688
    [5]
    潘国权, 梁亚峰, 林蓓蓓, 等. NGAL、KIM-1和IL-18三者联合检测在儿童急性肾损伤早期诊断中的意义[J].中国现代医生, 2015, 53(9): 67-69. http://d.wanfangdata.com.cn/Periodical/zwkjzlml-yyws201509021

    Pan GQ, Liang YF, Lin BB, et al. Significance of combined detection of NGAL, KIM-1 and IL-18 in the early diagnosis of acute kidney injury in children[J]. Chin Mod Doct, 2015, 53(9): 67-69. http://d.wanfangdata.com.cn/Periodical/zwkjzlml-yyws201509021
    [6]
    Ghonemy TA, Amro GM. Plasma neutrophil gelatinase-associated lipocalin (NGAL) and plasma cystatin C (CysC) as biomarker of acute kidney injury after cardiac surgery[J]. Saudi J Kidney Dis Transpl, 2014, 25(3):582-588. doi: 10.4103/1319-2442.132194
    [7]
    Ma L, Zhang H, Hu K, et al. The imbalance between Tregs, Th17 cells and inflammatory cytokines among renal transplant recipients[J]. BMC Immunol, 2015, 16:56. doi: 10.1186/s12865-015-0118-8
    [8]
    Araújo IR, Ferrari TC, Teixeira-Carvalho A, et al. Cytokine signature in infective endocarditis[J]. PLoS One, 2015, 10(7):e0133631. doi: 10.1371/journal.pone.0133631
    [9]
    O'Carroll SJ, Kho DT, Wiltshire R, et al. Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells[J]. J Neuroinflammation, 2015, 12:131. doi: 10.1186/s12974-015-0346-0
    [10]
    Yan C, Li XY, Li B, et al. Expression of Toll-like receptor (TLR) 2 and TLR4 in the livers of mice infected by Clonorchis sinensis[J]. J Infect Dev Ctries, 2015, 9(10):1147-1155. http://d.scholar.cnki.net/detail/SJPDTEMP_U/SJPD1CB0F0CD9C2E10EAC35FEC44E5CF2475
    [11]
    Zhang F, Pang N, Zhu Y, et al. CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients[J]. BMC Infect Dis, 2015, 15:457. doi: 10.1186/s12879-015-1156-9
    [12]
    Sada-Ovalle I, Ocaña-Guzman R, Pérez-Patrigeón S, et al. Tim-3 blocking rescue macrophage and T cell function against Mycobacterium tuberculosis infection in HIV+ patients[J]. J Int AIDS Soc, 2015, 18:20078.
    [13]
    Wyns H, Croubels S, Vandekerckhove M, et al. Multiplex analysis of pro-inflammatory cytokines in serum of Actinobacillus pleuropneumoniae-infected pigs[J]. Res Vet Sci, 2015, 102:45-48. doi: 10.1016/j.rvsc.2015.07.006
    [14]
    肖漓, 石炳毅, 何秀云, 等.流式细胞微球捕获技术检测肾移植受者外周血细胞因子的应用[J].中华检验医学杂志, 2010, 33(12):1128-1132. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZHYX201108003184.htm

    Li X, Shi BY, He XY, et al. Determination of cytokines in peripheral blood by cytometric bead array in kidney transplantation recipients[J].Chin J Lab Med, 2010, 33(12):1128-1132. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZHYX201108003184.htm
    [15]
    李锦霞, 杨炳华, 何杨, 等.流式微球技术检测血小板特异性抗体[J].临床检验杂志, 2010, 28(2):110-112. http://cdmd.cnki.com.cn/Article/CDMD-10285-2010152800.htm

    Li JX, Yang BH, He Y, et al. Detection of platelet-associated autoantibodies against platelet-specific receptors by cytometric bead assay[J]. Chin J Clin Lab Sci, 2010, 28(2):110-112. http://cdmd.cnki.com.cn/Article/CDMD-10285-2010152800.htm
    [16]
    凌云, 孔欣, 陈宝安.流式微球技术检测血小板特异性抗体的研究进展[J].中国实验血液学杂志, 2015, 23(4):1208-1211. http://www.cnki.com.cn/Article/CJFDTOTAL-XYSY201504066.htm

    Ling Y, Kong X, Chen BA. Research progress on cytometric bead assay for platelet antibody detection[J]. J Exp Hematol, 2015, 23(4):1208-1211. http://www.cnki.com.cn/Article/CJFDTOTAL-XYSY201504066.htm
    [17]
    肖昌彬, 孔维军, 刘秋桃, 等.流式微球技术在小分子物质高通量快速检测中的应用[J].中国中药杂志, 2015, 40(18):3515-3523. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201518001.htm

    Xiao CB, Kong WJ, Liu QT, et al. Application of flow microsphere technique in rapid detection of small molecular weight[J].Chin J Chin Mater Med, 2015, 40(18):3515-3523. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201518001.htm
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