Volume 7 Issue 1
Jan.  2016
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Qiu Xiaochen, Li Yuanxin, Liang Yumei, et al. Comparative study on the preservation quality of intestinal grafts from donors of donation after cardiac death and cadaveric donors[J]. ORGAN TRANSPLANTATION, 2016, 7(1): 26-31. doi: 10.3969/j.issn.1674-7445.2016.01.005
Citation: Qiu Xiaochen, Li Yuanxin, Liang Yumei, et al. Comparative study on the preservation quality of intestinal grafts from donors of donation after cardiac death and cadaveric donors[J]. ORGAN TRANSPLANTATION, 2016, 7(1): 26-31. doi: 10.3969/j.issn.1674-7445.2016.01.005
shu

Comparative study on the preservation quality of intestinal grafts from donors of donation after cardiac death and cadaveric donors

doi: 10.3969/j.issn.1674-7445.2016.01.005

  • Department of General Surgery, the 309th Hospital of People's Liberation Army, Beijing 100091, China

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  • Corresponding author: Li Yuanxin, E-mail:liyuanxin1966@163.com
  • Received Date: 2015-11-18
    Available Online: 2021-01-19
  • Publish Date: 2016-01-15
    Abstract
  •   Objective  To compare the preservation quality of intestinal grafts from donors of donation after cardiac death (DCD) and cadaveric donors.  Methods  Quality of intestinal grafts from 7 cadaveric donors(group N) and 7 DCD donors(group DCD) in Beijing from 2013 to 2014 was evaluated. The grafts were preserved after perfusion and resection, and then intestinal tissue was collected 30 min and 6 h later. Meanwhile, histopathological examination and intestinal graft injury score (Chiu's integral method) were performed. The content of malondialdehyde (MDA) in intestinal tissue was detected by thiobarbituric acid assay, and the apoptosis of intestinal mucosa cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method.  Results  Intestinal graft injury scores for group N and DCD were (1.46±0.81) and (1.76±0.21) respectively at 30 min after preservation, and (3.86±0.42) and(4.17±0.71), respectively at 6 h after preservation(both in P > 0.05). Compared with the preservation of 30 min, intestinal graft injury scores increased significantly in both groups at 6 h after preservation (both in P < 0.05). The contents of MDA in intestinal tissue of the small intestinal graft in group N and DCD were(100±10)pmol/mg and (110±13) pmol/mg, respectively at 30 min after preservation (P > 0.05), and (170±18) pmol/mg and (310±29) pmol/ mg, respectively at 6 h after preservation, of which the difference was statistically significant between the two groups at the same time(P < 0.05). Compared with the preservation of 30 min, the contents of MDA increased significantly in both groups at 6 h after preservation (both in P < 0.05). The number of apoptotic intestinal mucosal cells in small intestinal grafts for group N and DCD was (9.78±2.56) and (15.78±2.84), respectively at 30 min after preservation (P > 0.05), and (31.32±1.38) and (53.42±1.95), respectively at 6 h after preservation, of which the difference was statistically significant between the two groups (P < 0.05). Compared with the preservation of 30 min, the number of apoptotic intestinal mucosal cells in small intestinal grafts increased significantly in both groups at 6 h after preservation(P < 0.05).  Conclusions  Preservation quality of small intestinal grafts in DCD donors is roughly equivalent to that in traditional cadaveric donors, which suggests that small intestinal grafts in DCD donors may be used in clinical intestinal transplantation.

     

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    • References(18)
  • [1]
    O'Keefe SJ. Nutritional issues in the short bowel syndrome:total parenteral nutrition, enteral nutrition and the role of transplantation[J]. Nestle Nutr Inst Workshop Ser, 2015, 82:75-90. https://www.researchgate.net/publication/283543533_Nutritional_Issues_in_the_Short_Bowel_Syndrome_-_Total_Parenteral_Nutrition_Enteral_Nutrition_and_the_Role_of_Transplantation
    [2]
    Silva JT, San-Juan R, Fernandez-Caamano B, et al. Infectious complications following small bowel transplantation [J].Am J Transplant, 2015, DOI: 10.1111/ajt.13535 [Epub ahead of print].
    [3]
    霍枫.公民心脏死亡器官捐献开启我国器官移植新时代[J].器官移植, 2013, 4(5):247-249. http://www.organtranspl.com/browse/detail/qkid/29/id/265.html

    Huo F. New time of organ transplantation started by donation after cardiac death of citizens in China[J]. Organ Transplant, 2013, 4(5):247-249. http://www.organtranspl.com/browse/detail/qkid/29/id/265.html
    [4]
    邓斐文, 陈焕伟, 甄作均, 等.腹主动脉联合门静脉快速灌注法在器官捐献供体肝肾联合获取中的应用[J].器官移植, 2015, 6(1):51-54, 58. http://www.organtranspl.com/browse/detail/qkid/32/id/115.html

    Deng HW, Chen HW, Zhen ZJ, et al. Application of fast perfusion through abdominal aorta and portal vein in combined liver and kidney procurement from organ donation[J]. Organ Transplant, 2015, 6(1):51-54, 58. http://www.organtranspl.com/browse/detail/qkid/32/id/115.html
    [5]
    柳勤龙, 宋军, 高振明, 等.原位灌洗联合快速切取腹腔器官的临床应用(附56例报告)[J].大连医科大学学报, 2006, 28(5): 395-397. http://www.cnki.com.cn/Article/CJFDTOTAL-DLYK200605014.htm

    Liu QL, Song J, Gao ZM, et al. Clinical application of surgical technique for combined liver and kidney harvesting:a report of 56 cases[J].J Dalian Univ, 2006, 28(5): 395-397. http://www.cnki.com.cn/Article/CJFDTOTAL-DLYK200605014.htm
    [6]
    蔡金贞, 傅志仁, 朱志军, 等.肝肾等多脏器联合切取的手术方法改进[J].肝胆胰外科杂志, 2005, 17(3): 180-182. http://www.cnki.com.cn/Article/CJFDTOTAL-GDYW200503002.htm

    Cai JZ, Fu ZR, Zhu ZJ, et al. Improvement of surgical technique for combined liver and kidney harvesting in clinical practice[J]. J Hepatopancreatobiliary Surg, 2005, 17(3): 180-182. http://www.cnki.com.cn/Article/CJFDTOTAL-GDYW200503002.htm
    [7]
    Osaki S, Locher MR, Lushaj EB, et al. Functional evaluation of human donation after cardiac death donor hearts using a continuous isolated myocardial perfusion technique: potential for expansion of the cardiac donor population[J]. J Thorac Cardiovasc Surg, 2014, 148(3):1123-1130. doi: 10.1016/j.jtcvs.2014.06.050
    [8]
    Ouaedackers JS, Beuk RJ, Bennet L, et al. An evaluation of methods for grading histologic injury following ischemia perfusion of the small bowel[J]. Transplant Proc, 2000, 32(6):1307-1310. doi: 10.1016/S0041-1345(00)01238-0
    [9]
    Palocaren MS. An overview of intestine and multivisceral transplantation[J]. Crit Care Nurs Clin North Am, 2011, 23(3):457-469. doi: 10.1016/j.ccell.2011.08.003
    [10]
    袁小鹏, 韩明, 王小平, 等.心脏死亡器官捐献供者肾移植29例报告[J].肾脏病与透析肾移植杂志, 2013, 22(4):336-339. http://www.cnki.com.cn/Article/CJFDTOTAL-SZBY201304006.htm

    Yuan XP, Han M, Wang XP, et al. Kidney transplantation from cardiac death donor: a report of 29 cases[J]. Chin J Nephrol Dial Transplant, 2013, 22(4):336-339. http://www.cnki.com.cn/Article/CJFDTOTAL-SZBY201304006.htm
    [11]
    马俊杰, 李光辉, 徐璐, 等.零点活组织检查冷冻切片组织病理学半定量评分评估DCD供肾损伤的意义[J].中华器官移植杂志, 2013, 34(10):582-586. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201310002

    Ma JJ, Li GH, Xu L, et al. Histopathological semi-quantitative evaluation of kidney injury of donation after cardiac death by cryosections of zero biopsy:a double centre and control clinical trial[J]. Chin J Organ Transplant, 2013, 34(10):582-586. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201310002
    [12]
    薛武军, 田普训, 项和立, 等.心脏死亡器官捐献供肾移植单中心60例经验总结[J].中华器官移植杂志, 2013, 34(7):387-391. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201307002

    Xue WJ, Tian PX, Xiang HL, et al. Donation after cardiac death donor renal transplantation: a single center 60 cases of DCD experience[J]. Chin J Organ Transplant, 2013, 34(7):387-391. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201307002
    [13]
    Cobianchi L, Zonta S, Vigano J, et al. Experimental small bowel transplantation from non-heart-beating donors: a large-animal study[J]. Transplant Proc, 2009, 41(1):55-56. doi: 10.1016/j.transproceed.2008.08.151
    [14]
    李元新, 李幼生, 李民.尸体供者小肠、肝和肾脏联合切取和保存技术[J/CD].中华移植杂志(电子版), 2010, 4(4): 277-284.

    Li YX, Li YS, Li M. Techniques for procurement and preservation of intestine, liver and kidneys grafts from the same cadaveric donor[J/CD]. Chin J Transplant(Electr Edit), 2010, 4(4): 277-284.
    [15]
    Jonecová Z, TóthŠ, Varga J, et al. The immediate response of jejunal mucosa to small bowel heterotopic allotransplatation in rats[J]. Tissue Cell, 2014, 46(1):21-26. doi: 10.1016/j.tice.2013.08.004
    [16]
    Wong YL, Lautenschläger I, Dombrowsky H, et al. Hydroxyethyl starch (HES 130/0.4) impairs intestinal barrier integrity and metabolic function:findings from a mouse model of the isolated perfused small intestine[J]. PLoS One, 2015, 10(3):e0121497. doi: 10.1371/journal.pone.0121497
    [17]
    Jiang LL, Zhang JJ, Zhang ZZ, et al. Effect of intraperitoneal resuscitation with different concentrations of sodium pyruvate on intestinal ischemia reperfusion injury in hemorrhagic shock rat[J]. Shock, 2015, DOI: 10.1097/SHK.0000000000000515 [Epub ahead of print].
    [18]
    Jonecova Z, Toth S, Maretta M, et al. Protective effect of ischemic preconditioning on the jejunal graft mucosa injury during cold preservation[J]. Exp Mol Pathol, 2015, 99(2):229-235. doi: 10.1016/j.yexmp.2015.06.020
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