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Zhang Lei, Chen Zheng, Zhang Yirui, et al. Application of ImmuKnow immune cell function assay in monitoring of immune function after renal transplantation[J]. ORGAN TRANSPLANTATION, 2015, 6(6): 429-433. doi: 10.3969/j.issn.1674-7445.2015.06.017
Citation: Zhang Lei, Chen Zheng, Zhang Yirui, et al. Application of ImmuKnow immune cell function assay in monitoring of immune function after renal transplantation[J]. ORGAN TRANSPLANTATION, 2015, 6(6): 429-433. doi: 10.3969/j.issn.1674-7445.2015.06.017
shu

Application of ImmuKnow immune cell function assay in monitoring of immune function after renal transplantation

doi: 10.3969/j.issn.1674-7445.2015.06.017

  • Department of Organ Transplantation, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China

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  • Corresponding author: Pan Guanghui, Email:panguanghui@126.com
  • Received Date: 2015-09-21
    Available Online: 2021-01-19
  • Publish Date: 2015-11-15
    Abstract
  •   Objective  To investigate the application value of ImmuKnow immune cell function assay in monitoring of immune function changes after renal transplantation.  Methods  One hundred and six patients with uraemia undergoing renal transplantation in the Department of Organ Transplantation of the Second Affiliated Hospital of Guangzhou Medical University from January 2013 to December 2014 were included. Blood specimens were collected before transplantation and at the occurrence of infection or acute rejection during 12 months after transplantation. ImmoKnow was used to determine the adenosine triphosphate(ATP) content in CD4+T cells. The ATP content of patients with renal transplantation at different clinical conditions were observed and compared, including periopreative group, stable group, acute rejection group and infecticn group(including severe pneumonia). The ratio of T cell subsets (CD4+T cells, CD8+T cells) and natural killer (NK) cells in peripheral blood were detected. Pearson correlation analysis was used to detect the association between ATP and the blood trough concentration of tacrolimus (FK506) and ciclosporin (CsA).  Results  The ATP content of the patients in the infection group was lower than that of the patients in the stable group (P < 0.001). The ATP content of patients with severe pneumonia was lower than that of patients with other infections (P < 0.05). The percentage of CD4+T cells of the patients in the infection group was lower than that of the patients in the postoperative stable group (P < 0.05). The ATP content was not associated with the postoperative blood trough concentration of FK506 and CsA.  Conclusions  ImmuKnow assay may be used to monitor the postoperative immune function of patients after renal transplantation. The detection of ATP content in CD4+T cells has hinting and pre-warning function for postoperative infection, especially for severe pneumonia.

     

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    • References(17)
  • [1]
    Zeevi A, Lunz J. Cylex ImmuKnow cell function assay[J]. Methods Mol Biol, 2013, 1034:343-351. doi: 10.1007/978-1-62703-493-7
    [2]
    薛武军, 田晓辉, 刘华, 等.检测肾移植受者CD4+T淋巴细胞内三磷腺苷的意义[J].器官移植, 2010, 1(1):41-44. http://www.organtranspl.com/browse/detail/qkid/44/id/640.html

    Xue WJ, Tian XH, Liu H, et al. Applications of the examination for CD4+ T cells' ATP in renal transplant recipient[J]. Organ Transplant, 2010, 1(1):41-44. http://www.organtranspl.com/browse/detail/qkid/44/id/640.html
    [3]
    Kowalski RJ, Post DR, Mannon RB, et al. Assessing relative risks of infection and rejection:a meta-analysis using an immune function assay[J]. Transplantation, 2006, 82(5):663-668. doi: 10.1097/01.tp.0000234837.02126.70
    [4]
    王宇成, 王敏敏, 茅幼英, 等.免疫细胞功能分析在监测肾移植受者免疫状态中的应用[J].中华肾脏病杂志, 2014, 30(5):345-350. http://cdmd.cnki.com.cn/Article/CDMD-10335-1011057982.htm

    Wang YC, Wang MM, Mao YY, et al. Application of immune cell functional assay in monitoring immune status in renal transplant recipients[J].Chin J Nephrol, 2014, 30(5):345-350. http://cdmd.cnki.com.cn/Article/CDMD-10335-1011057982.htm
    [5]
    Kowalski R, Post D, Schneider MC, et al. Immune cell function testing:an adjunct to therapeutic drug monitoring in transplant patient management[J]. Clin Transplant, 2003, 17(2):77-88. doi: 10.1034/j.1399-0012.2003.00013.x
    [6]
    Kobashigawa JA, Kiyosaki KK, Patel JK, et al. Benefit of immune monitoring in heart transplant patients using ATP production in activated lymphocytes[J]. J Heart Lung Transplant, 2010, 29(5):504-508. doi: 10.1016/j.healun.2009.12.015
    [7]
    Husain S, Raza K, Pilewski JM, et al. Experience with immune monitoring in lung transplant recipients:correlation of low immune function with infection[J]. Transplantation, 2009, 87(12):1852-1857. doi: 10.1097/TP.0b013e3181a75ad2
    [8]
    Cabrera R, Ararat M, Soldevila-Pico C, et al. Using an immune functional assay to differentiate acute cellular rejection from recurrent hepatitis C in liver transplant patients[J]. Liver Transpl, 2009, 15(2):216-222. doi: 10.1002/lt.v15:2
    [9]
    Bhorade SM, Janata K, Vigneswaran WT, et al. Cylex ImmuKnow assay levels are lower in lung transplant recipients with infection[J]. J Heart Lung Transplant, 2008, 27(9):990-994. doi: 10.1016/j.healun.2008.06.005
    [10]
    王庆华, 原欣翔, 王瑾, 等.细胞免疫能量测定在肾移植中的应用[J].中华器官移植杂志, 2010, 31(4):231-233.

    Wang QH, Yuan XX, Wang J, et al. Application of Immuknow immune cell function assay in kidney transplantation[J]. Chin J Organ Transplant, 2010, 31(4):231-233.
    [11]
    孙煦勇, 秦科, 农江, 等.肝肾联合移植术后T细胞亚群变化及其共刺激分子的表达[J].中华实验外科杂志, 2012, 29(10):1934-1937. http://d.wanfangdata.com.cn/periodical_zhsywk201210020.aspx

    Sun XY, Qin K, Nong J, et al. Changes in T lymphocytic subpopulations and expression of co-stimulatory molecules after combined liver and kidney transplantation[J]. Chin J Exp Surg, 2012, 29(10):1934-1937. http://d.wanfangdata.com.cn/periodical_zhsywk201210020.aspx
    [12]
    张辉, 何军, 李扬, 等. Immuknow法监测细胞免疫功能在诊断肾移植术后感染中的应用价值[J].中华泌尿外科杂志, 2013, 34(7):538-541. doi: 10.3760/cma.j.issn.1000-6702.2013.07.018

    Zhang H, He J, Li Y, et al. Application value of cell function assay using the Immuknow in infections after renal transplantation[J]. Chin J Urol, 2013, 34(7):538-541. doi: 10.3760/cma.j.issn.1000-6702.2013.07.018
    [13]
    Tada H, Tsuchiya N, Satoh S, et al. Impact of CYP3A5 and MDR1(ABCB1) C3435T polymorphisms on the pharmacokinetics of tacrolimus in renal transplant recipients[J]. Transplant Proc, 2005, 37(4):1730-1732. doi: 10.1016/j.transproceed.2005.02.073
    [14]
    刘军, 唐丽洁, 包瑾芳, 等. ImmuKnow免疫细胞功能监测在狼疮性肾炎并发感染中的价值及感染相关危险因素的初步分析[J].中华医学杂志, 2014, 94(6):424-427. http://med.wanfangdata.com.cn/paper/detail/periodicalpaper_zhyx201406007

    Liu J, Tang LJ, Bao JF, et al. Infection in lupus nephritis:risk factors and significance of immune cell functional assay[J].Natl Med J China, 2014, 94(6):424-427. http://med.wanfangdata.com.cn/paper/detail/periodicalpaper_zhyx201406007
    [15]
    Natsuda K, Soyama A, Takatsuki M, et al. The efficacy of the ImmuKnow assay for evaluating the immune status in human immunodeficiency virus and hepatitis C virus-coinfected patients[J]. Transplant Proc, 2014, 46(3):733-735. doi: 10.1016/j.transproceed.2013.11.119
    [16]
    Andrikopoulou E, Mather PJ. Current insights:use of Immuknow in heart transplant recipients[J]. Prog Transplant, 2014, 24(1):44-50. doi: 10.7182/pit2014664
    [17]
    Jo Y, Lim J, Kim Y, et al. CD4 T-cell function using Cylex Immu Know and lymphocyte subset recovery folloving allogeneic hematopoietic stem cell transplantation[J]. Transpl Immunol, 2015, 33(2):78-83. doi: 10.1016/j.trim.2015.09.001
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