Volume 5 Issue 5
Sep.  2014
Turn off MathJax
Article Contents
Article Navigation >  ORGAN TRANSPLANTATION  > 2014  >  5(5): 294-298
Ma Shuaijun, Zhang Geng, Cao Zhiqiang, et al. Relationship between the expression of SDF-1, ICAM-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury[J]. ORGAN TRANSPLANTATION, 2014, 5(5): 294-298. doi: 10.3969/j.issn.1674-7445.2014.05.007
Citation: Ma Shuaijun, Zhang Geng, Cao Zhiqiang, et al. Relationship between the expression of SDF-1, ICAM-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury[J]. ORGAN TRANSPLANTATION, 2014, 5(5): 294-298. doi: 10.3969/j.issn.1674-7445.2014.05.007
shu

Relationship between the expression of SDF-1, ICAM-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury

doi: 10.3969/j.issn.1674-7445.2014.05.007

  • Department of Urology, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China

  • Received Date: 2014-05-10
  • Publish Date: 2014-09-15
    Abstract
  •   Objective   To investigate the relationship between the expression of stromal cell derived factor (SDF)-1, intercellular adhesion molecule (ICAM)-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury (IRI).   Methods   Sixty Sprague-Dawley (SD) rats were randomly divided into operation group and sham operation group with 30 rats in each group. Then each group was divided into 6 subgroups (1 h,6 h,12 h,24 h, 48 h or 72 h) according to different time of measurement after operation with 5 rats in each subgroup. Renal IRI mode1s were built in rats of operation group. The bilateral renal arteries were dissected in rats of sham operation group and then the incision was sutured. The renal function, renal tubular necrosis score and the variation of SDF-1, ICAM-1 expression in renal tissues at different time points were measured. The relationship between the expression of SDF-1, ICAM-1 in renal tissues and renal tubular necrosis score in rats of operation group was analyzed by Pearson correlation analysis.   Results   The levels of urea nitrogen (BUN) and serum creatinine (Scr) after operation in each subgroup of operation group were significantly higher than those before operation and those in the corresponding subgroups of sham operation group(all in P<0.05). They increased significantly 12 h after operation and reached the peak at 48 h after operation. The renal tubular necrosis score in operation group increased gradually over time (all in P<0.05). The highest renal tubular necrosis score was in the 48 h operation subgroup (P<0.05). Compared with those in 1 h operation subgroup, the expression of SDF-1, ICAM-1 in rats' renal tissues of 6 h operation subgroup began to increase significantly, and they reached the peak at 48 h after operation and began to drop down at 72 h after operation. The expression of SDF-1, ICAM-1 in rats renal tissues in operation group were positively correlated with the levels of BUN, Scr and renal tubular necrosis score at different time points after operation (r=0.614, 0.662, 0.751;0.640, 0.703, 0.785;P<0.05).   Conclusion   When rat's renal tissue develops IRI, the expression of SDF-1, ICAM-1, BUN, Scr and renal tubular necrosis score increased.The expression of SDF-1, ICAM-1 are positively correlated with BUN, Scr and renal tubular necrosis score. The increased expression of SDF-1, ICAM-1 can serve as an indicator of the severity of renal IRI.

     

    • FullText(HTML)
  • loading
    • References(20)
  • [1]
    Kłoda K, Domański L, Pawlik A, et al. The impact of ICAM1 and VCAM1 gene polymorphisms on chronic allograft nephropathy and transplanted kidney function[J]. Transplant Proc,2013,45(6):2244-2247. doi: 10.1016/j.transproceed.2013.03.043
    [2]
    Kłoda K, Domański L, Pawlik A, et al. The impact of ICAM1 and VCAM1 gene polymorphisms on long-term renal transplant function and recipient outcomes[J]. Ann Transplant,2013,18:231-237. doi: 10.12659/AOT.883917
    [3]
    Mannam VK, Lewis RE, Cruse JM. The fate of renal allografts hinges on responses of the microvascular endothelium[J]. Exp Mol Pathol,2013,94(2):398-411. doi: 10.1016/j.yexmp.2012.06.002
    [4]
    Cho HK, Kim SY, Seong JK, et al. Hepatitis B virus X increases immune cell recruitment by induction of chemokine SDF-1[J]. FEBS Lett,2014,588(5):733-739. doi: 10.1016/j.febslet.2014.01.017
    [5]
    Flameng W, De Visscher G, Mesure L, et al. Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricularoutflow tract reconstruction eliminates immune response-related degeneration[J]. J Thorac Cardiovasc Surg,2014,147(4):1398-1404. doi: 10.1016/j.jtcvs.2013.06.022
    [6]
    易小敏, 张更, 马帅军. 大鼠急性肾缺血再灌注损伤模型的建立与评估[J]. 现代生物医学进展,2011, 11(21): 4027-4029. http://www.cnki.com.cn/Article/CJFDTOTAL-SWCX201121008.htm

    Yi XM, Zhang G, Ma SJ. Establishment and evaluation of rat acute kidney ischemia/reperfusion model[J]. Prog Mod Biomed,2011, 11(21): 4027-4029. http://www.cnki.com.cn/Article/CJFDTOTAL-SWCX201121008.htm
    [7]
    Paller MS, Hoidal JR, Ferris TF. Oxygen free radicals in ischemic acute renal failure in the rat[J]. J Clin Invest,1984,74(4):1156-1164. doi: 10.1172/JCI111524
    [8]
    Shayegi N, Meyer C, Lambert K, et al. CXCR4 blockade and sphingosine-1-phosphate activation facilitate engraftment of haematopoietic stem and progenitor cells in a non-myeloablative transplant model[J]. Br J Haematol,2014,164(3):409-413. doi: 10.1111/bjh.2014.164.issue-3
    [9]
    Yang D, Sun S, Wang Z, et al. Stromal cell-derived factor-1 receptor CXCR4-overexpressing bone marrow mesenchymal stem cells accelerate wound healing by migrating into skin injury areas[J]. Cell Reprogram,2013,15(3):206-215. http://cn.bing.com/academic/profile?id=65100295&encoded=0&v=paper_preview&mkt=zh-cn
    [10]
    Xu X, Zhu F, Zhang M, et al. Stromal cell-derived factor-1 enhances wound healing through recruiting bone marrow-derived mesenchymalstem cells to the wound area and promoting neovascularization[J]. Cells Tissues Organs,2013,197(2):103-113. doi: 10.1159/000342921
    [11]
    Xargay-Torrent S, López-Guerra M, Montraveta A, et al. Sorafenib inhibits cell migration and stroma-mediated bortezomib resistance by interfering B-cell receptor signaling and protein translation in mantle cell lymphoma[J]. Clin Cancer Res,2013,19(3):586-597. doi: 10.1158/1078-0432.CCR-12-1935
    [12]
    Wang S, Cheng H, Dai G, et al. Umbilical cord mesenchymal stem cell transplantation significantly improves neurological function in patients with sequelae of traumatic brain injury[J]. Brain Res,2013,1532:76-84. doi: 10.1016/j.brainres.2013.08.001
    [13]
    Wang L, Pasha Z, Wang S, et al. Protein kinase G1α overexpression increases stem cell survival and cardiac function after myocardial infarction[J]. PLoS One,2013,8(3):e60087. doi: 10.1371/journal.pone.0060087
    [14]
    Virani S, Edwards AK, Thomas R, et al. Blocking of stromal cell-derived factor-1 reduces neoangiogenesis in human endometriosis lesions in a mouse model[J]. Am J Reprod Immunol,2013,70(5):386-397. http://cn.bing.com/academic/profile?id=2064946686&encoded=0&v=paper_preview&mkt=zh-cn
    [15]
    Tadakuma K, Tanaka N, Haraguchi Y, et al. A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage[J]. Biomaterials,2013,34(36):9018-9025. doi: 10.1016/j.biomaterials.2013.08.006
    [16]
    Sun D, Narsinh K, Wang H, et al. Effect of autologous bone marrow mononuclear cells transplantation in diabetic patients with ST-segmentelevation myocardial infarction[J]. Int J Cardiol,2013,167(2):537-547. doi: 10.1016/j.ijcard.2012.01.068
    [17]
    Suga A, Ueda K, Takemoto Y, et al. Significant role of bone marrow-derived cells in compensatory regenerative lung growth[J]. J Surg Res,2013,183(1):84-90. doi: 10.1016/j.jss.2012.12.013
    [18]
    Stojanovic D, Cvetkovic T, Stojanovic M, et al. Crosstalk of inflammatory mediators and lipid parameters as early markers of renal dysfunction in stable renal transplant recipients with regard to immunosuppression[J]. Ann Transplant,2013,18:414-423. doi: 10.12659/AOT.889239
    [19]
    张力,赵雪云,刘胜春,等. 蛋白酶体抑制剂MG132减轻大鼠移植胰腺缺血再灌注损伤的作用[J].中华器官移植杂志,2013,34(6):358-361.

    Zhang L, Zhao XY, Liu SC, et al. Protective effect of MG132 on ischemia-reperfusion injury after pancreaticoduodenal transplantation in rats[J]. Chin J Organ Transplant,2013,34(6):358-361.
    [20]
    王烨铭,陈静瑜,童继春,等.乌司他丁减轻无心跳大鼠供肺缺血再灌注损伤的作用及机制[J].中华器官移植杂志,2013,34(8):498-502.

    Wang YM, Chen JY, Tong JC, et al. Protective effects of ulinastatin on ischemia-reperfusion injury during rat non-heart beating donor lung transplantation[J]. Chin J Organ Transplant,2013,34(8):498-502.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Tables(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (111) PDF downloads(104) Cited by()
    Proportional views
    Related

    WeChat Subscription Account

    Wechat Service Account

    Contact Us
    • Tel：020-38736410
    • Email: organtranspl@163.com
    • Address：The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou
    • Editorial Office of Organ Transplantation  粤ICP备2021030844号
    Links

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return