Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10

Wei Yuxiang; Wang Xiaogang; Xiao Li; Zhou Wenqiang; Zheng Dehua; Yu Tao; Shi Bingyi

doi:10.3969/j.issn.1674-7445.2014.04.009

Volume 5 Issue 4

Jul. 2014

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Wei Yuxiang, Wang Xiaogang, Xiao Li, et al. Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10[J]. ORGAN TRANSPLANTATION, 2014, 5(4): 237-241. doi: 10.3969/j.issn.1674-7445.2014.04.009

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Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10

doi: 10.3969/j.issn.1674-7445.2014.04.009

Organ Transplant Institute, Beijing Key Organ Transplantation and Immune Regulation Laboratory, the 309^th Hospital of Chinese People's Liberation Army, Beijing 100091, China

Received Date: 2014-05-08
Available Online: 2021-01-19
Publish Date: 2014-07-15

Abstract

Abstract

Objective To investigate the feasibility of immature dendritic cells (imDC) phagocytized psoralen ultraviolet A (PUVA)-treated splenic lymphocytes (PUVA-SP DC) in mice inducing B lymphocytes to be regulatory B cells(Breg) with high secretion of interleukin(IL)-10(IL-10⁺Breg). Methods Bone marrow-derived DC of mice was cultured. Spleen lymphocytes of mice were isolated and treated by PUVA, and turned to be PUVA-SP. The bone marrow-derived imDC was co-cultured with PUVA-SP in vitro to obtain PUVA-SP DC. Splenic B lymphocytes of mice were separated by anti-CD19 magnetic beads and co-cultured with different kinds of DC for 48 hours. The levels of interferon(IFN)-γ, transforming growth factor(TGF)-β, IL-12p70, and IL-10 in the culture supernatant of B lymphocytes, imDC, imDC+B lymphocytes, PUVA-SP DC and PUVA-SP DC+B lymphocytes were measured by enzyme-linked immune absorbent assay(ELISA). The accounts of IL-10⁺Breg in B lymphocytes, imDC+B lymphocytes, mDC+B lymphocytes and PUVA-SP DC+B lymphocytes were detected by flow cytometry. Results Compared with the other 4 groups, the level of IL-10 in cell culture supernatant of PUVA-SP DC+B lymphocytes was significantly higher(all in P<0.05). Compared with the other groups, the account of IL-10⁺Breg in PUVA-SP DC+B lymphocytes was significantly higher. Conclusions PUVA-SP DC can induce splenic B lymphocytes to differentiate into IL-10⁺Breg.
- Dendritic cell,
- Photochemical method in vitro,
- Regulatory B cell,
- Interleukin-10

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Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10

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Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10

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