Modified technique of renal transplantation model in rats

Li Cai; Zhu Yi; Ye Shaojun; Zhou Wei; Liu Hong; Niu Ying; She Xingguo; Ming Yingzi

doi:10.3969/j.issn.1674-7445.2014.03.007

Volume 5 Issue 3

May 2014

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Li Cai, Zhu Yi, Ye Shaojun, et al. Modified technique of renal transplantation model in rats[J]. ORGAN TRANSPLANTATION, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007

Citation:

Li Cai, Zhu Yi, Ye Shaojun, et al. Modified technique of renal transplantation model in rats[J]. ORGAN TRANSPLANTATION, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007

Citation:

Li Cai, Zhu Yi, Ye Shaojun, et al. Modified technique of renal transplantation model in rats[J]. ORGAN TRANSPLANTATION, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007

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Modified technique of renal transplantation model in rats

doi: 10.3969/j.issn.1674-7445.2014.03.007

Department of Transplantation, the Third Xiangya Hospital, Central South University, Changsha 410013, China

Received Date: 2014-02-10
Available Online: 2021-01-19
Publish Date: 2014-05-15

Abstract

Abstract

Objective To investigate modified technique of renal transplantation model in rats. Methods Sprague-Dawley rats were selected as donors (n=21) and Wistar rats as recipients (n=42). Renal allografts of both sides were harvested from the donors for renal transplantation. After resection of left kidney, end-to-end anastomosis of renal arteries between donor and recipient was performed by the assistance of home-made catheter. And end-to-end anastomosis between recipient's renal vein and donor's inferior vena cava was also performed. The donor's ureter with bladder patch was anastomosed to the recipient's bladder. Finally the right kidney was removed, cefminox(10 mg) was injected intraperitoneally, and then the abdominal cavity was closed. The operation data were recorded, including the operation time, artery and vein anastomosis time, cold and warm ischemia time and so on. Living for 3 days after operation was regarded as a success model. The success rate of modeling was calculated and the cause of death was analyzed. Results The operation time of donor was (32.7±5.6) min, and repair time for kidney was (4.2±1.1) min. The operation time of recipient was (42.3±42.3) min, including (10.1±3.2) min of the artery anastomosis time, (13.9±2.5) min of vein anastomosis time, (6.3±1.4) min of urinary tract reconstruction time. Warm ischemia time was (5.4±1.8) s, and cold ischemia time was (56.2±7.3) min. In 42 recipient rats, 40 rats were successful modeling and the success rate was 95.2%. Two rats died. One died of artery anastomosis hemorrhage, and the other died of diffuse peritonitis caused by urine leakage. Conclusions Renal transplantation model in rats with modified vascular end-to-end anastomosis has the characters of simple handling, short operation time and high success rate.
- Renal transplantation,
- Animal model,
- Rat,
- Surgery

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References

[1]	李衡, 纳宁, 黄正宇, 等.促红细胞生成素对大鼠移植肾缺血-再灌注损伤的防护作用[J].器官移植, 2013, 4(5):263-267, 273. http://www.organtranspl.com/browse/detail/qkid/66/id/267.html Li H, Na N, Huang ZY, et al. Protective effect of erythropoietin on graft ischemia-reperfusion injury after renal transplantation in rats[J]. Organ Transplant, 2013, 4(5):263-267, 273. http://www.organtranspl.com/browse/detail/qkid/66/id/267.html
[2]	Karatzas T, Santiago S, Xanthos T, et al. An easy and safe model of kidney transplantation in rats[J]. Microsurgery, 2007, 27(8):668-672. doi: 10.1002/(ISSN)1098-2752
[3]	袁清, 洪善娟, 蔡明, 等.水溶性一氧化碳释放分子抑制供肾树突状细胞活化减轻移植后排斥反应[J].中华器官移植杂志, 2012, 33(8):462-465. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZHYX201210002318.htm Yuan Q, Hong SJ, Cai M, et al. Water-soluble CO-releasing molecules inhibit activation of donor renal dendritic cells and suppress graft rejection[J]. Chin J Organ Transplant, 2012, 33(8):462-465. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZHYX201210002318.htm
[4]	王金国, 臧崇森, 王远涛, 等.大鼠肾移植急性排斥模型的建立[J].中国实验诊断学, 2010, 14(3):369-371. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSZD201003018.htm Wang JG, Zang CS, Wang YT, et al. The establishment of renal acute rejection model in rats[J]. Chin J Lab Diagn, 2010, 14(3):369-371. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSZD201003018.htm
[5]	Jayarajan S, Daller JA. Novel mouse kidney transplantation technique: waste not, want not[J]. J Surg Res, 2011, 168(2):175-176. doi: 10.1016/j.jss.2010.06.008
[6]	Timsit MO, Ge X, Yuan X, et al. Kidney transplantation in the rat: an institutional technique[J].Prog Urol(法文), 2011, 21(3):173-176. doi: 10.1016/j.purol.2010.06.005
[7]	邓新军, 刘永光, 郭颖, 等.大鼠右侧供肾移植中肾静脉吻合方式的比较研究[J].中华器官移植杂志, 2012, 33(12):746-749. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201212012 Deng XJ, Liu YG, Guo Y, et al. A comparison study on venous anastomosis for right donor kidney transplantation in rats[J]. Chin J Organ Transplant, 2012, 33(12):746-749. http://d.wanfangdata.com.cn/Periodical/zhqgyz98201212012
[8]	刘皎林, 周洪澜, 王医术, 等.改良的血管端端吻合法建立大鼠原位肾移植模型[J].中国实验诊断学, 2012, 16(2):229-232. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSZD201202015.htm Liu JL, Zhou HL, Wang YS, et al. Modified method of vascular anastomosis in rat model of orthotopic kidney transplantation[J]. Chin J Lab Diagn, 2012, 16(2):229-232. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSZD201202015.htm
[9]	Hoeger S, Fontana J, Jarczyk J, et al. Vagal stimulation in brain dead donor rats decreases chronic allograft nephropathy in recipients[J]. Nephrol Dial Transplant, 2014, 29(3):544-549. doi: 10.1093/ndt/gft451
[10]	Zarjou A, Guo L, Sanders PW, et al. A reproducible mouse model of chronic allograft nephropathy with vasculopathy[J]. Kidney Int, 2012, 82(11):1231-1235. doi: 10.1038/ki.2012.277
[11]	Erickson L, Wynn C, Pan F, et al. Microarray gene expression profiling of chronic allograft nephropathy in the rat kidney transplant model[J]. Transpl Immunol, 2012, 27(2/3):75-82. http://cn.bing.com/academic/profile?id=1974046468&encoded=0&v=paper_preview&mkt=zh-cn
[12]	Yang CC, Chen KH, Hsu SP, et al. Augmented renal prostacyclin by intrarenal bicistronic cyclo-oxygenase-1/prostacyclin synthase gene transfer attenuates renal ischemia-reperfusion injury[J]. Transplantation, 2013, 96(12):1043-1051. doi: 10.1097/TP.0b013e3182a77e52
[13]	Garcia-Cenador MB, Lorenzo-Gomez MF, Herrero-Payo JJ, et al. Cardiotrophin-1 administration protects from ischemia-reperfusion renal injury and inflammation[J]. Transplantation, 2013, 96(12):1034-1042. doi: 10.1097/TP.0b013e3182a74db4
[14]	Zhou S, Li E, He J, et al. Staged microvascular anastomosis training program for novices: transplantation of both kidneys from one rat donor[J]. Chin Med J, 2014, 127(4):712-717. http://cn.bing.com/academic/profile?id=2426379894&encoded=0&v=paper_preview&mkt=zh-cn
[15]	Lin M, Li L, Li L, et al. The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis[J]. BMC Complement Altern Med, 2014, 14(1):19. doi: 10.1186/1472-6882-14-19
[16]	Xie LB, Zeng DY, Wang XD, et al. Preconditioning with gabexate is superior to inosine for ameliorating acute renal ischemia-reperfusion injury in rats[J]. Transplant Proc, 2014, 46(1):40-45. doi: 10.1016/j.transproceed.2013.10.037
[17]	Neri F, Puviani L, Tsivian M, et al. Protective effect of an inhibitor of interleukin-8(meraxin) from ischemia and reperfusion injury in a rat model of kidney transplantation[J]. Transplant Proc, 2007, 39(6):1771-1772. doi: 10.1016/j.transproceed.2007.05.018

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Modified technique of renal transplantation model in rats

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