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大鼠双供体肝移植模型的改进

王丹 陈亚惊 马延龄 刘小龙 王博方 汪学艳 陈昊

王丹, 陈亚惊, 马延龄, 等. 大鼠双供体肝移植模型的改进[J]. 器官移植, 2020, 11(1): 72-75, 103. doi: 10.3969/j.issn.1674-7445.2020.01.011
引用本文: 王丹, 陈亚惊, 马延龄, 等. 大鼠双供体肝移植模型的改进[J]. 器官移植, 2020, 11(1): 72-75, 103. doi: 10.3969/j.issn.1674-7445.2020.01.011
Wang Dan, Chen Yajing, Ma Yanling, et al. Improvement of dual liver transplantation rat model[J]. ORGAN TRANSPLANTATION, 2020, 11(1): 72-75, 103. doi: 10.3969/j.issn.1674-7445.2020.01.011
Citation: Wang Dan, Chen Yajing, Ma Yanling, et al. Improvement of dual liver transplantation rat model[J]. ORGAN TRANSPLANTATION, 2020, 11(1): 72-75, 103. doi: 10.3969/j.issn.1674-7445.2020.01.011

大鼠双供体肝移植模型的改进

doi: 10.3969/j.issn.1674-7445.2020.01.011
基金项目: 

国家自然科学基金 81670594

国家自然科学基金 81470791

国家自然科学基金 81376597

详细信息
    作者简介:

    王丹,男,1994年生,硕士研究生,研究方向为器官移植和消化系肿瘤,Email:1215125895@qq.com

    通讯作者:

    陈昊,男,教授,博士研究生导师,研究方向为器官移植和消化系肿瘤,Email:chenhaodrs@163.com

  • 中图分类号: R617

Improvement of dual liver transplantation rat model

More Information
  • 摘要:   目的  建立改良的大鼠双供体肝移植模型。  方法  在经典供体Y型双髂静脉再通双侧肝移植物和受体门静脉和胆管的基础上,通过增加大鼠体质量、右侧移植物增加右下叶、适度的胆管长度、修剪Y型血管、“三角形法”吻合的方法,改进大鼠双供体肝移植模型。记录双供体肝移植手术时间、冷缺血时间、热缺血时间及无肝期; 观察受体的术后并发症发生情况; 分析受体术后7、30 d的存活情况。  结果  大鼠双供体肝移植的手术时间为(114±7)min,冷缺血时间为(36±3)min,热缺血时间为(9.7±1.6)min,无肝期为(19.9±2.2)min。受体大鼠术后并发症发生率为31%(5/16),包括2例腹腔积液、1例出血、1例胆漏、1例呼吸梗阻。受体大鼠术后7、30 d生存率分别为81%(13/16)、56%(9/16)。  结论  改良后的大鼠双供体肝移植技术,可以建立稳定的大鼠双供体肝移植模型,值得推广应用。

     

  • 图  1  供体获取的手术过程

    注:左侧移植物为右上叶,右侧移植物为右上叶+右下叶。

    Figure  1.  The surgical procedure of donor acquisition

    图  2  复合供体的肝上下腔静脉与受体下腔静脉吻合

    Figure  2.  Suprahepatic vena cava of the composite donors were anastomosed with inferior vena cava of the recipient

    图  3  双供体肝移植大鼠的生存曲线

    Figure  3.  Survival curves of double-donor liver transplantation rats

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出版历程
  • 收稿日期:  2019-10-15
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2020-01-15

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