Comparison of blood glucose-lowering function of transplant islets between subcutaneous adipose tissues of inguinal region and renal capsule in mice
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Abstract:
Objective To compare the effect of transplant islets between the subcutaneous inguinal white adipose tissues and renal capsule in the treatment of type 1 diabetes mellitus in mouse models. Methods The mice with type 1 diabetes mellitus undergoing islet transplantation were divided into the white adipose group (n=10) and renal capsule group (n=10). The islets were isolated, purified and transplanted to the subcutaneous white adipose tissues of inguinal region and renal capsule. The random blood glucose level and glucose tolerance function of the recipient mice in two groups were continuously monitored after operation. Islet grafts of the surviving recipient mice were harvested at postoperative 100 d for histopathological examination. Results In the white adipose group, the blood glucose levels of 6 recipient mice were restored to normal at 1 month after transplantation, whereas the blood glucose levels of the other 4 recipient mice were high, which died before the end of monitoring. In the renal capsule group, the blood glucose levels of 10 recipient mice returned to normal within 10 d after transplantation. Islet grafts of the recipient mice in two groups could lower the blood glucose levels, whereas the islet grafts in the white adipose group required a longer time to exert the effect. The glucose tolerance function of the mice in the renal capsule group was significantly better than that of those in white adipose group (P < 0.05). Histopathological examination demonstrated that the insulin of the islet grafts was normally expressed in two groups. Conclusions The islets transplanted into the subcutaneous white adipose tissues of inguinal region can play an effective role in regulating the changes of blood glucose level. Although the blood glucose-lowering function is slightly weaker than that of the islets graft in the renal capsule, it has multiple advantages resembling the ideal islet transplantation sites, which is a promising replacement site for islet transplantation. -
图 1 两组受体小鼠的胰岛移植位点
A图为将胰岛移植到小鼠左腿腹股沟皮下白色脂肪组织; B图为将胰岛移植到小鼠肾被膜下
Figure 1. Islet transplantation sites in two groups of recipient mice
图 2 两组受体小鼠胰岛移植后不同时间点的血糖水平
Figure 2. The blood glucose level at different time points after islet transplantation in two groups of recipient mice
图 3 两组受体小鼠胰岛移植后的糖耐量功能曲线
与肾被膜组比较, aP < 0.05，bP < 0.001
Figure 3. The glucose tolerance function curves after islet transplantation in two groups of recipient mice
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[1] OGURTSOVA K, DA ROCHA FERNANDES JD, HUANG Y, et al. IDF diabetes atlas: global estimates for the prevalence of diabetes for 2015 and 2040[J]. Diabetes Res Clin Pract, 2017, 128:40-50. DOI: 10.1016/j.diabres.2017.03.024. [2] WANG L, GAO P, ZHANG M, et al. Prevalence and ethnic pattern of diabetes and prediabetes in China in 2013[J]. JAMA, 2017, 317(24):2515-2523. DOI: 10.1001/jama.2017.7596. [3] 赵振平, 李镒冲, 王丽敏, 等. 2013年中国成人糖尿病前期的地理分布及相关因素分析[J].中华预防医学杂志, 2018, 52(2):158-164. DOI:10.3760/cma.j.issn.0253- 9624.2018.02.008.ZHAO ZP, LI YC, WANG LM, et al. Geographical variation and related factors in prediabetes prevalence in Chinese adults in 2013[J]. Chin J Prev Med, 2018, 52(2):158-164. DOI: 10.3760/cma.j.issn.0253-9624.2018.02.008. [4] MAFFI P, SECCHI A. Islet transplantation alone versus solitary pancreas transplantation: an outcome-driven choice?[J]. Curr Diab Rep, 2019, 19(5):26. DOI: 10.1007/s11892-019-1145-2. [5] BANDEIRAS C, HWA AJ, CABRAL JMS, et al. Economics of beta-cell replacement therapy[J]. Curr Diab Rep, 2019, 19(9):75. DOI: 10.1007/s11892-019-1203-9. [6] SHAPIRO AM, LAKEY JR, RYAN EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen[J]. N Engl J Med, 2000, 343(4):230-238. doi: 10.1056/NEJM200007273430401 [7] BAIDAL DA, RICORDI C, BERMAN DM, et al. Bioengineering of an intraabdominal endocrine pancreas[J]. N Engl J Med, 2017, 376(19):1887-1889. DOI: 10.1056/NEJMc1613959. [8] ANAZAWA T, OKAJIMA H, MASUI T, et al. Current state and future evolution of pancreatic islet transplantation[J]. Ann Gastroenterol Surg, 2018, 3(1):34-42. DOI: 10.1002/ags3.12214. [9] BOTTINO R, KNOLL MF, KNOLL CA, et al. The future of islet transplantation is now[J]. Front Med (Lausanne), 2018, 5:202. DOI: 10.3389/fmed.2018.00202. [10] MAFFI P, NANO R, MONTI P, et al. Islet allotransplantation in the bone marrow of patients with type 1 diabetes: a pilot randomized trial[J]. Transplantation, 2019, 103(4):839-851. DOI: 10.1097/TP.0000000000002416. [11] STICE MJ, DUNN TB, BELLIN MD, et al. Omental pouch technique for combined site islet autotransplantation following total pancreatectomy[J]. Cell Transplant, 2018, 27(10):1561-1568. DOI: 10.1177/0963689718798627. [12] YASUNAMI Y, NAKAFUSA Y, NITTA N, et al. A novel subcutaneous site of islet transplantation superior to the liver[J]. Transplantation, 2018, 102(6):945-952. DOI: 10.1097/TP.0000000000002162. [13] EKSER B, VAGEFI PA. Search for the best site in islet xenotransplantation[J]. Int J Surg, 2019, 70:106-107. DOI: 10.1016/j.ijsu.2019.08.001. [14] 杨娜, 张化冰, 许岭翎. 1型糖尿病的非胰岛素降糖药物治疗[J].中国医刊, 2019, 54(4):352-355. DOI: 10.3969/j.issn.1008-1070.2019.04.002.YANG N, ZHANG HB, XU LL. Non-insulin hypoglycemic agents for type 1 diabetes[J]. Chin J Med, 2019, 54(4):352-355. DOI: 10.3969/j.issn.1008-1070.2019.04.002. [15] PATHAK V, PATHAK NM, O' NEILL CL, et al. Therapies for type 1 diabetes: current scenario and future perspectives[J]. Clin Med Insights Endocrinol Diabetes, 2019, 12: 1179551419844521. DOI: 10.1177/1179551419844521. [16] NICLAUSS N, MEIER R, BÉDAT B, et al. Beta-cell replacement: pancreas and islet cell transplantation[J]. Endocr Dev, 2016, 31:146-162. DOI: 10.1159/000439412. [17] STOKES RA, CHENG K, LALWANI A, et al. Transplantation sites for human and murine islets[J]. Diabetologia, 2017, 60(10):1961-1971. DOI: 10.1007/s00125-017-4362-8. [18] ADDISON P, FATAKHOVA K, RODRIGUEZ RILO HL. Considerations for an alternative site of islet cell transplantation[J]. J Diabetes Sci Technol, 2019: 1932296819868495. DOI: 10.1177/1932296819868495. [19] SAKATA N, YOSHIMATSU G, KODAMA S. The spleen as an optimal site for islet transplantation and a source of mesenchymal stem cells[J]. Int J Mol Sci, 2018, 19(5): E1391. DOI: 10.3390/ijms19051391. [20] 尹注增, 刘荣.胰岛移植部位的选择及优缺点[J/CD].实用器官移植电子杂志, 2016, 4(6): 355-359. DOI: 10.3969/j.issn.2095-5332.2016.06.007.YIN ZZ, LIU R. Selection and advantages and disadvantages of islet transplantation site[J/CD]. Pract J Organ Transplant(Electr Vers), 2016, 4(6): 355-359. DOI: 10.3969/j.issn.2095-5332.2016.06.007. [21] SAKATA N, AOKI T, YOSHIMATSU G, et al. Strategy for clinical setting in intramuscular and subcutaneous islet transplantation[J]. Diabetes Metab Res Rev, 2014, 30(1):1-10. DOI: 10.1002/dmrr.2463. [22] LU Y, ZOU S, BERTERA S, et al. A method for islet transplantation to the omentum in mouse[J]. J Vis Exp, 2019 (143). DOI: 10.3791/57160. [23] WEAVER JD, HEADEN DM, AQUART J, et al. Vasculogenic hydrogel enhances islet survival, engraftment, and function in leading extrahepatic sites[J]. Sci Adv, 2017, 3(6):e1700184. DOI: 10.1126/sciadv.1700184. [24] MCCOY MG, SEO BR, CHOI S, et al. Collagen I hydrogel microstructure and composition conjointly regulate vascular network formation[J]. Acta Biomater, 2016, 44:200-208. DOI: 10.1016/j.actbio.2016.08.028. -
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