建立小鼠腹部心脏移植模型联合尾静脉注射的实践体会（附视频）

鲍志野; 朱嘉亿; 蹇骞; 潘崎; 刘博千; 张景旭; 赵柯艺; 易才彧; 刘浩

doi:10.3969/j.issn.1674-7445.2019.02.009

建立小鼠腹部心脏移植模型联合尾静脉注射的实践体会（附视频）

doi: 10.3969/j.issn.1674-7445.2019.02.009

110001 沈阳，中国医科大学附属第一医院肝胆外科暨器官移植科辽宁省器官移植重点实验室

基金项目:

国家自然科学基金项目 81373173

详细信息

作者简介:
鲍志野，男，1982年生，本科，主治医师，研究方向为移植免疫，Email：nobledafeiyu1989@163.com

通讯作者:
刘浩，男，1973年生，博士，副主任医师，副教授，研究方向为移植免疫和肝癌免疫，Email：liuhaodoctor@hotmail.com

中图分类号: R617, R-332
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出版历程
- 收稿日期: 2018-12-24
- 网络出版日期: 2021-01-19
- 刊出日期: 2019-03-15

Practice experience of establishment of abdominal heart transplantation model combined with tail vein injection in mice (with video demonstration)

Department of Hepatobiliary Surgery, the First Hospital of China Medical University, Key Laboratory of Organ Transplantation of Liaoning Province, Shenyang 110001, China

More Information

Corresponding author: Liu Hao, Email:liuhaodoctor@hotmail.com

摘要

摘要: 目的总结建立稳定的小鼠腹部心脏移植模型联合尾静脉注射的实践体会。方法实验练习包括50对供、受体为昆明小鼠的同系移植，40对供、受体为C57BL/6J小鼠的同系移植；正式实验包括10对供、受体为C57BL/6J小鼠的同系移植，30对以Balb/c小鼠为供体、C57BL/6J小鼠为受体的同种异系移植。记录手术过程中每个步骤的时间（包括供体心脏摘取和修整时间、受体血管吻合等）。术后每日观察移植心脏搏动持续时间及受体存活时间，同时记录移植小鼠的尾静脉注射所需时间。同系移植术后30 d、同种异系移植术后7 d行移植心脏病理学检查（各5只）。结果正式实验心脏移植成功率为90%。供体心脏摘取和修整时间为（13.9±0.6）min，受体冷缺血时间为（14.2±1.2）min，血管吻合时间为（34.2±3.1）min，总手术时间为（86.6±5.4）min，术后同系移植小鼠移植心脏存活时间达到100 d以上，术后30 d病理学检查显示仅有少量炎症细胞浸润。同种异系移植小鼠因发生排斥反应，存活时间为（7.2±0.5）d。术后7 d病理学检查示心肌大量炎症细胞浸润，呈急性细胞性排斥反应表现。尾静脉注射超过200次后，成功率达90%。结论本研究成功建立了稳定的小鼠腹部心脏移植模型。同时进行尾静脉注射可有效地利用预实验的小鼠。
- 腹部心脏移植 /
- 心脏移植 /
- 尾静脉注射 /
- 小鼠 /
- 移植免疫 /
- 器官移植 /
- 排斥反应 /
- 炎症细胞
Abstract: Objective To summarize the practice experience of establishing a stable abdominal heart transplantation model combined with tail vein injection in mice. Methods In the preliminary experiment, 50 pairs of donor and recipient Kunming mice received isotransplantation, 40 pairs of donor and recipient C57BL/6J mice underwent isotransplantation. In the formal experiment, 10 pairs of donor and recipient C57BL/6J mice received isotransplantation, 30 pairs of Balb/c mice as the donor and C57BL/6J mice as the recipient received allotransplantation. The time of each step of the heart transplantation (including harvesting and dressing of the donor heart, vascular anastomosis of the recipient, etc.) was recorded. The duration of transplanted heart beat and the survival time of the recipient was observed daily after operation. The time required for tail vein injection in the transplanted mice was recorded. Pathological examination of the transplanted heart was performed at 30 d after isotransplantation (n=5) and 7 d after allotransplantation (n=5). Results In the formal experiment, the success rate of heart transplantation was 90%. The harvesting and dressing time of donor heart was (13.9±0.6) min. The cold ischemia time of the recipient was (14.2±1.2) min. The vascular anastomosis time was (34.2±3.1) min. The total operation time was (86.6±5.4) min. Postoperatively, the transplanted heart of the mice undergoing isotransplantation survived longer than 100 d. Pathological examination at postoperative 30 d demonstrated only a slight amount of inflammatory cell infiltration. The survival time of the mice receiving allotransplantation was (7.2±0.5) d due to rejection reaction. At postoperative 7 d, pathological examination showed a large quantity of inflammatory cells infiltrating into the myocardium, manifested with acute cellular rejection. The success rate reached 90% after over 200 times of tail vein injection. Conclusions In this study, a stable mouse abdominal heart transplantation model is successfully established. The mouse models in the preliminary experiment can be utilized for tail vein injection.
- Abdominal heart transplantation /
- Heart transplantation /
- Tail vein injection /
- Mouse /
- Transplantation immunity /
- Organ transplantation /
- Rejection reaction /
- Inflammatory cell

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图 1 小鼠腹部心脏移植术后心脏组织病理学检查（HE，×100）

A图示同系移植术后30 d心肌组织变化；B图示同种异系移植术后7 d心肌组织变化

Figure 1. Pathological examination of heart tissue after abdominal heart transplantation in mice

下载: 全尺寸图片幻灯片

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