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摘要:
目的 探讨使用他克莫司（FK506）的大鼠体内短链脂肪酸（SCFA）的变化及其对血糖水平的影响。 方法 将10只SD大鼠分为FK506组和对照组，每组各5只。FK506组给予每日皮下注射FK506（3 mg/kg）+含10%乙醇的葵花油溶液，持续4周；对照组给予皮下注射等量的含10%乙醇的葵花油溶液，持续4周。给药期间每周测量2组大鼠体质量。给药结束后，检测两组大鼠的血糖水平、血液和粪便中的SCFA含量。 结果 与对照组比较，第2、3、4周FK506组大鼠的相对体质量均降低，差异均有统计学意义（均为P < 0.01）。与对照组比较，FK506组大鼠在给予葡萄糖后0、30、60 min时血糖水平均升高，差异均有统计学意义（P < 0.01～0.05）。与对照组比较，FK506组大鼠粪便中乙酸、丙酸、异丁酸、丁酸、异戊酸和戊酸含量均降低，差异均有统计学意义（P < 0.01～0.05）。 结论 FK506可导致大鼠血糖水平升高，这种升血糖的作用可能由大鼠粪便SCFA含量降低引起。 Abstract:Objective To investigate the changes of short chain fatty acids (SCFA) induced by tacrolimus (FK506) in rats and evaluate its effect on blood glucose levels. Methods Ten SD rats were divided into the FK506 group and control group (n=5 in each group). In the FK506 group, the rats were received a subcutaneous injection of FK506 (3 mg/kg) +sunflower oil solution containing 10% ethanol daily for consecutive 4 weeks. In the control group, the rats were received a subcutaneous injection of an equivalent amount of sunflower oil solution containing 10% ethanol for consecutive 4 weeks. During the drug injection period, the body mass of rats was measured every week in two groups. After the drug injection period, blood glucose level, SCFA content in the blood and feces samples were measured in two groups. Results Compared with the control group, the relative body mass of rats in the FK506 group was significantly lower at the 2nd, 3rd and 4th weeks (all P < 0.01). Compared with the control group, the blood glucose levels of rats in the FK506 group were significantly increased at 0, 30, and 60 min after giving glucose (P < 0.01-0.05). Compared with the control group, the contents of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid and valeric acid in the feces sample were significantly lower in the FK506 group (P < 0.01-0.05). Conclusions FK506 can upregulate the blood glucose level in rats, which is probably induced by the decrease of SCFA content in rat feces. -
Key words:
- Rat /
- Tacrolimus /
- Blood glucose /
- Short chain fatty acids /
- Body mass /
- Post-transplant diabetes mellitus /
- Gut flora
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图 1 两组大鼠给药不同时间相对体质量的变化
与第1周比较，aP < 0.05，bP < 0.01
Figure 1. Changes of relative body weight of rats in two groups at different dosing times
图 2 两组大鼠给药4周后血糖水平变化
与对照组比较，aP < 0.05，bP < 0.01
Figure 2. Changes of blood glucose levels of rats in two groups after 4 weeks of dosing
图 3 两组大鼠血液中SCFA含量的比较
Figure 3. Comparison of SCFA content in rat blood between two groups
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