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高通量测序分析肝移植急性排斥反应相关基因单核苷酸多态性位点的突变

鲍远大 易述红

鲍远大, 易述红. 高通量测序分析肝移植急性排斥反应相关基因单核苷酸多态性位点的突变[J]. 器官移植, 2018, 9(3): 205-210. doi: 10.3969/j.issn.1674-7445.2018.03.007
引用本文: 鲍远大, 易述红. 高通量测序分析肝移植急性排斥反应相关基因单核苷酸多态性位点的突变[J]. 器官移植, 2018, 9(3): 205-210. doi: 10.3969/j.issn.1674-7445.2018.03.007
Bao Yuanda, Yi Shuhong. High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation[J]. ORGAN TRANSPLANTATION, 2018, 9(3): 205-210. doi: 10.3969/j.issn.1674-7445.2018.03.007
Citation: Bao Yuanda, Yi Shuhong. High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation[J]. ORGAN TRANSPLANTATION, 2018, 9(3): 205-210. doi: 10.3969/j.issn.1674-7445.2018.03.007

高通量测序分析肝移植急性排斥反应相关基因单核苷酸多态性位点的突变

doi: 10.3969/j.issn.1674-7445.2018.03.007
基金项目: 

广东省自然科学基金 2016A030313224

广东省省级产业技术研究与开发专项资金项目 20169013

详细信息
    作者简介:

    鲍远大,男,1992年生,硕士研究生,研究方向为肝移植,Email:1013261488@qq.com

    通讯作者:

    易述红,男,1973年生,博士,主任医师,研究方向为肝移植,Email:yishuhong@163.com

  • 中图分类号: R617, R318.04

High-throughput sequencing analysis of single nucleotide polymorphism loci mutation in genes associated with acute rejection in liver transplantation

More Information
  • 摘要:   目的  利用高通量测序分析肝移植术后急性排斥反应相关度最高的基因单核苷酸多态性(SNP)位点的突变情况。  方法  收集同种异体原位肝移植术的68例受体外周血样本,根据有否发生急性排斥反应分为急性排斥组(13例)和非排斥组(55例)。通过查阅文献,最终确定与排斥反应发生相关的44个SNP突变位点。以44个SNP位点作为检测靶点,用高通量测序分析对两组受体外周血样本进行检测,经生物信息学分析出与急性排斥反应发生相关基因SNP位点的突变率。  结果  急性排斥组中的白细胞介素(IL)-10TT基因型、T等位基因、AA基因型、A等位基因的SNP位点突变率明显高于非排斥组;急性排斥组中的细胞趋化因子受体(CCR5AG基因型的SNP位点突变率明显低于非排斥组,CCR5 GG基因型的SNP位点突变率明显高于非排斥组;急性排斥组中的IL-4 CT基因型的SNP位点突变率明显高于非排斥组,IL-4 TT基因型的SNP位点突变率明显低于非排斥组;急性排斥组中核因子-κB抑制因子α(NF-κBIA)C等位基因的SNP位点突变率明显高于非排斥组;急性排斥组中维生素D受体(VDR)CC基因型和C等位基因的SNP位点突变率明显低于非排斥组,差异均有统计学意义(均为P < 0.05)。  结论  高通量测序分析发现肝移植术后急性排斥反应相关基因中,其SNP位点突变率较高的包括IL-10 TT基因型、T等位基因、AA基因型、A等位基因,CCR5 GG基因型、AG基因型,IL-4 CT基因型、TT基因型,NF-κBIA C等位基因,VDR CC基因型和C等位基因。

     

  • 表  1  两组肝移植受体SNP基因型分析

    Table  1.   SNP genotype analysis of liver transplant recipients in two groups

    基因 SNP rs号 基因型 突变率[n(%)] χ2 P
    急性排斥组(n=13) 非排斥组(n=55)
    IL-10 592C/T rs1800872 TT 9(69) 21(38) 4.11 < 0.05
          TG 4(31) 27(49) 1.42 > 0.05
          GG 0 7(13) 1.84 > 0.05
          T等位基因 22(85) 69(63) 4.55 < 0.05
          G等位基因 4(15) 41(37)    
    IL-10 819T/C rs1800871 AA 9(69) 21(38) 4.11 < 0.05
          AG 4(31) 27(49) 1.42 > 0.05
          GG 0 7(13) 1.84 > 0.05
          A等位基因 22(85) 69(63) 4.55 < 0.05
          G等位基因 4(15) 41(37)    
    CCR5 2459A/G rs1799987 AA 2(15) 6(11) 0.20 > 0.05
          AG 2(15) 30(55) 6.47 < 0.05
          GG 9(69) 19(35) 5.22 < 0.05
          A等位基因 6(23) 42(38) 2.10 > 0.05
          G等位基因 20(77) 68(62)    
    NF-κBIA 126G/A rs696 CC 8(62) 19(35) 3.20 > 0.05
          CT 5(39) 26(47) 0.33 > 0.05
          TT 0 10(18) 2.77 > 0.05
          C等位基因 21(81) 64(58) 4.58 < 0.05
          T等位基因 5(19) 46(42)    
    IL-4 590C/T rs2243250 CC 0 2(4) - > 0.05
          CT 9(69) 16(29) 5.66 < 0.05
          TT 4(31) 37(67) 5.85 < 0.05
          C等位基因 9(35) 20(18) 3.39 > 0.05
          T等位基因 17(65) 90(82)    
    VDR ApaI T > G rs7975232 CC 3(23) 32(58) 5.19 < 0.05
          CA 6(46) 19(35) 0.61 > 0.50
          AA 4(31) 4(7) 3.56 > 0.05
          C等位基因 12(46) 83(75) 8.57 < 0.05
          A等位基因 14(54) 27(25)    
      -为无χ2
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-03-05
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2018-05-15

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