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体外光分离置换疗法对皮肤移植小鼠IL-12p70和Th1/Th2类细胞因子产生的影响

魏玉香 肖漓 蔡明 钱叶勇 于涛 石炳毅

魏玉香, 肖漓, 蔡明, 等. 体外光分离置换疗法对皮肤移植小鼠IL-12p70和Th1/Th2类细胞因子产生的影响[J]. 器官移植, 2017, 8(5): 355-359. doi: 10.3969/j.issn.1674-7445.2017.05.004
引用本文: 魏玉香, 肖漓, 蔡明, 等. 体外光分离置换疗法对皮肤移植小鼠IL-12p70和Th1/Th2类细胞因子产生的影响[J]. 器官移植, 2017, 8(5): 355-359. doi: 10.3969/j.issn.1674-7445.2017.05.004
Wei Yuxiang, Xiao Li, Cai Ming, et al. Effect of extracorporeal photopheresis on the expression of IL-12p70 and Th1/Th2-like cytokines in mouse models with skin allograft[J]. ORGAN TRANSPLANTATION, 2017, 8(5): 355-359. doi: 10.3969/j.issn.1674-7445.2017.05.004
Citation: Wei Yuxiang, Xiao Li, Cai Ming, et al. Effect of extracorporeal photopheresis on the expression of IL-12p70 and Th1/Th2-like cytokines in mouse models with skin allograft[J]. ORGAN TRANSPLANTATION, 2017, 8(5): 355-359. doi: 10.3969/j.issn.1674-7445.2017.05.004

体外光分离置换疗法对皮肤移植小鼠IL-12p70和Th1/Th2类细胞因子产生的影响

doi: 10.3969/j.issn.1674-7445.2017.05.004
基金项目: 

国家自然科学基金 81370578

详细信息
    通讯作者:

    石炳毅,Email:shibingyi@medmail.com.cn

  • 中图分类号: R617, R392.4, R-332

Effect of extracorporeal photopheresis on the expression of IL-12p70 and Th1/Th2-like cytokines in mouse models with skin allograft

More Information
    Corresponding author: Shi Bingyi, Email: shibingyi@medmail.com.cn
  • 摘要:   目的  探讨输注体外光分离置换疗法处理的脾淋巴细胞对皮肤移植小鼠白细胞介素(IL)-12p70和辅助性T细胞(Th)1/Th2类细胞因子产生的影响。  方法  以C57BL/6小鼠为供体,BALB/c小鼠为受体,建立小鼠皮肤移植模型。分离C57BL/6和BALB/c小鼠脾淋巴细胞(CSP、BSP),制备经8-甲氧基补骨脂素联合长波紫外线(PUVA)处理的小鼠脾淋巴细胞(PUVA-SP)。根据受体静脉输注的成分将实验动物随机分为5组(每组12只):PUVA-BSP组、PUVA-CSP组、BSP组、CSP组及磷酸盐缓冲液(PBS)对照组,每组受体分别于术前7 d、手术当日和术后7 d按组别从尾静脉注入PUVA-BSP、PUVA-CSP、BSP、CSP或PBS。观察经PUVA处理脾淋巴细胞凋亡情况,检测受体小鼠外周血中IL-12p70和Th1/Th2类细胞因子产生的情况。  结果  移植术后,PUVA-BSP组和PUVA-CSP组小鼠外周血的IL-12p70表达水平明显低于BSP组、CSP组和PBS对照组(均为P < 0.01);PUVA-BSP组和PUVA-CSP组的Th1类细胞因子IL-2、干扰素(IFN)-γ表达水平均明显低于BSP组、CSP组和PBS对照组(均为P < 0.01);PUVA-BSP组和PUVA-CSP组的Th2类细胞因子IL-10表达水平明显高于BSP组、CSP组和PBS对照组(均为P < 0.01)。  结论  输注足够数量的PUVA-SP可诱导受体体内低表达IL-12p70,并且诱导产生Th2免疫偏移。

     

  • 图  1  各组小鼠外周血IL-12p70和Th1/Th2类细胞因子含量

    Figure  1.  The contents of IL-12p70 and Th1/Th2 cytokines in peripheral blood of mice in different groups

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出版历程
  • 收稿日期:  2017-06-24
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2017-09-15

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