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不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究

廖涛 赵大强 李思雯 韩飞 罗子寰 刘潇楠 孙启全

廖涛, 赵大强, 李思雯, 等. 不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究[J]. 器官移植, 2017, 8(3): 205-208. doi: 10.3969/j.issn.1674-7445.2017.03.006
引用本文: 廖涛, 赵大强, 李思雯, 等. 不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究[J]. 器官移植, 2017, 8(3): 205-208. doi: 10.3969/j.issn.1674-7445.2017.03.006
Liao Tao, Zhao Daqiang, Li Siwen, et al. Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation[J]. ORGAN TRANSPLANTATION, 2017, 8(3): 205-208. doi: 10.3969/j.issn.1674-7445.2017.03.006
Citation: Liao Tao, Zhao Daqiang, Li Siwen, et al. Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation[J]. ORGAN TRANSPLANTATION, 2017, 8(3): 205-208. doi: 10.3969/j.issn.1674-7445.2017.03.006

不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究

doi: 10.3969/j.issn.1674-7445.2017.03.006
基金项目: 

教育部博士点基金新教师类 20130171120100

国家自然科学基金面上项目 81470978

广东省自然科学基金重点项目 2015A030311040

详细信息
    通讯作者:

    孙启全,Email:sunqiq@mail.sysu.edu.cn

  • 中图分类号: R617, R392.4

Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation

More Information
  • 摘要:   目的   比较不同品系小鼠间皮肤移植致敏后抗供体特异性抗体(DSA)的变化特点。   方法   将小鼠分为Balb/c→C57BL/6皮肤移植组(6对)和Balb/c→C3H皮肤移植组(6对)。皮肤移植后收集d0、d2、d4、d7、d13、d17、d28、d35、d42、d49、d56时间点血清并检测DSA-IgG和DSA-IgM。   结果   小鼠皮肤移植致敏后1周内DSA-IgG水平升高不明显,1~4周内IgG水平持续快速增长,4~8周IgG水平处于高峰平台稳定。皮肤移植后8周内DSA-IgM无明显变化。Balb/c→C57BL/6皮肤移植组DSA-IgG水平明显低于Balb/c→C3H组,两组在d2、d17、d28、d35、d42、d49和d56时间点的IgG水平差异有统计学意义(均为P < 0.05),两组DSA-IgM在各时间点比较差异无统计学意义(均为P>0.05)。   结论   利用Balb/c→C3H适当提前皮肤移植后的肾移植手术时间,或改用免疫反应性更弱的Balb/c→C57BL/6组合有望建立排斥反应更弱的移植肾AMR模型。

     

  • 图  1  两组小鼠皮肤移植术后各个时间点的DSA-IgG、IgM变化趋势

    Figure  1.  Change trend of DSA-IgG and IgM of mice in two groups at each time point after skin transplantation

    表  1  两组小鼠皮肤移植术后各时间点DSA-IgG水平的比较

    Table  1.   Comparison of DSA-IgG levels of mice between two groups after skin transplantation at each time point (x ± s, ×104)

    时间点nBalb/c→C3H组Balb/c→C57BL/6组P
    d061.98±0.131.83±0.140.104
    d262.15±0.181.72±0.100.000
    d461.93±0.231.76±0.190.173
    d762.26±0.411.97±0.450.260
    d1363.57±0.273.14±0.640.153
    d1764.10±0.643.09±0.810.038
    d2867.79±1.294.42±1.690.003
    d3567.78±1.274.11±1.550.001
    d4267.85±2.204.06±1.600.007
    d4967.37±0.854.12±1.860.003
    d5668.17±3.454.02±1.380.021
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出版历程
  • 收稿日期:  2017-02-20
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2017-05-15

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