Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation
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摘要:
目的 比较不同品系小鼠间皮肤移植致敏后抗供体特异性抗体(DSA)的变化特点。 方法 将小鼠分为Balb/c→C57BL/6皮肤移植组(6对)和Balb/c→C3H皮肤移植组(6对)。皮肤移植后收集d0、d2、d4、d7、d13、d17、d28、d35、d42、d49、d56时间点血清并检测DSA-IgG和DSA-IgM。 结果 小鼠皮肤移植致敏后1周内DSA-IgG水平升高不明显,1~4周内IgG水平持续快速增长,4~8周IgG水平处于高峰平台稳定。皮肤移植后8周内DSA-IgM无明显变化。Balb/c→C57BL/6皮肤移植组DSA-IgG水平明显低于Balb/c→C3H组,两组在d2、d17、d28、d35、d42、d49和d56时间点的IgG水平差异有统计学意义(均为P < 0.05),两组DSA-IgM在各时间点比较差异无统计学意义(均为P>0.05)。 结论 利用Balb/c→C3H适当提前皮肤移植后的肾移植手术时间,或改用免疫反应性更弱的Balb/c→C57BL/6组合有望建立排斥反应更弱的移植肾AMR模型。 -
关键词:
- 皮肤移植 /
- 供体特异性抗体(DSA) /
- 抗体介导的排斥反应(AMR) /
- 小鼠 /
- 免疫反应 /
- 肾移植 /
- 动物模型 /
- 免疫球蛋白(Ig)
Abstract:Objective To compare the change features of anti-donor specific antibody (DSA) in different species of sentitized mice after skin transplantation. Methods All mice were divided into the Balb/c→C57BL/6 (6 pairs) and Balb/c→C3H skin transplantation groups (6 pairs). At d0, d2, d4, d7, d13, d17, d28, d35, d42, d49 and d56 after skin transplantation, the serum sample was prepared for detection of DSA-IgG and DSA-IgM levels. Results Moderate increase was noted in the DSA-IgG level in the sensitized mice within 1 week after skin transplantation. The IgG level was significantly increased within 1-4 week and peaked and stabilized within 4-8 week. No significant variation was observed in the DSA-IgM level at 8 weeks after skin transplantation. In the Balb/c→C57BL/6 skin transplantation group, the DSA-IgG level was significantly lower than that in the Balb/c→C3H group. Statistical significance was identified in the IgG levels between two groups at d2, d17, d28, d35, d42, d49 and d56 after skin transplantation (all P < 0.05). No statistical significance was noted in the DSA-IgM levels between two groups at each time point (all P>0.05). Conclusion Advancing the time of renal transplantation after skin transplantation moderately in the Balb/c→C3H group, or changing to the lower immunoreactive combination of Balb/c→C57BL/6 are aimed to establish AMR mouse models with mild rejection reaction. -
表 1 两组小鼠皮肤移植术后各时间点DSA-IgG水平的比较
Table 1. Comparison of DSA-IgG levels of mice between two groups after skin transplantation at each time point (x ± s, ×104)
时间点 n Balb/c→C3H组 Balb/c→C57BL/6组 P值 d0 6 1.98±0.13 1.83±0.14 0.104 d2 6 2.15±0.18 1.72±0.10 0.000 d4 6 1.93±0.23 1.76±0.19 0.173 d7 6 2.26±0.41 1.97±0.45 0.260 d13 6 3.57±0.27 3.14±0.64 0.153 d17 6 4.10±0.64 3.09±0.81 0.038 d28 6 7.79±1.29 4.42±1.69 0.003 d35 6 7.78±1.27 4.11±1.55 0.001 d42 6 7.85±2.20 4.06±1.60 0.007 d49 6 7.37±0.85 4.12±1.86 0.003 d56 6 8.17±3.45 4.02±1.38 0.021 -
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