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抗RANTES单抗联合环孢素诱导小鼠二次移植心脏长期存活

黄建 罗增荣 庄嘉伟 单忠贵 林链凤

黄建, 罗增荣, 庄嘉伟, 等. 抗RANTES单抗联合环孢素诱导小鼠二次移植心脏长期存活[J]. 器官移植, 2016, 7(6): 467-472. doi: 10.3969/j.issn.1674-7445.2016.06.011
引用本文: 黄建, 罗增荣, 庄嘉伟, 等. 抗RANTES单抗联合环孢素诱导小鼠二次移植心脏长期存活[J]. 器官移植, 2016, 7(6): 467-472. doi: 10.3969/j.issn.1674-7445.2016.06.011
Huang Jian, Luo Zengrong, Zhuang Jiawei, et al. Induction of long-term heart survival after secondary transplantation by anti-RANTES monoclonal antibody combined with ciclosporin in mouse models[J]. ORGAN TRANSPLANTATION, 2016, 7(6): 467-472. doi: 10.3969/j.issn.1674-7445.2016.06.011
Citation: Huang Jian, Luo Zengrong, Zhuang Jiawei, et al. Induction of long-term heart survival after secondary transplantation by anti-RANTES monoclonal antibody combined with ciclosporin in mouse models[J]. ORGAN TRANSPLANTATION, 2016, 7(6): 467-472. doi: 10.3969/j.issn.1674-7445.2016.06.011

抗RANTES单抗联合环孢素诱导小鼠二次移植心脏长期存活

doi: 10.3969/j.issn.1674-7445.2016.06.011
基金项目: 

厦门市科技计划项目 3502Z20134001

详细信息
    通讯作者:

    林链凤. E-mail:linlf5333@126.com.

  • 中图分类号: R617

Induction of long-term heart survival after secondary transplantation by anti-RANTES monoclonal antibody combined with ciclosporin in mouse models

More Information
  • 摘要:   目的   探讨抗RANTES单克隆抗体(单抗)联合环孢素(CsA)在抗小鼠心脏二次心脏移植排斥反应中的作用。   方法   以BALB/c小鼠为供体,C57BL/6小鼠为受体构建小鼠心脏二次移植模型。按随机数字表法分为4组:对照组(生理盐水组)、实验A组(抗RANTES单抗治疗组)、实验B组(CsA治疗组)及实验C组(抗RANTES单抗+CsA联合治疗组),每组6只。观察各组小鼠二次移植心脏存活时间;应用组织病理学观察各组移植心脏急性排斥反应的程度;采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测移植心脏组织中RANTES、白细胞介素(IL)-2、IL-10、干扰素(IFN)-γ和转录生长因子(TGF)-β基因的信使核糖核酸(mRNA)的相对表达量;采用酶链免疫吸附试验(ELISA)检测RANTES、IFN-γ、IL-2、IL-10和TGF-β在血清中的含量。   结果   所有实验小鼠二次心脏移植全部成功复跳。与对照组比较,实验A、B、C组的移植心脏存活时间明显延长(均为P<0.01)。移植心脏病理学染色可见,实验C组炎症细胞的浸润较对照组、实验A组、实验B组明显减少。与其它3组比较,实验C组的移植心脏组织RANTES、IFN-γ、IL-2的mRNA表达量均明显减少,而IL-10、TGF-β的mRNA表达量均明显增加(均为P<0.05)。与其它3组比较,实验C组的血清中RANTES、IL-2、IFN-γ含量均明显降低,而血清中IL-10、TGF-β含量均明显升高(均为P<0.05)。   结论   联合应用抗RANTES单抗和CsA可更有效诱导小鼠对二次心脏移植的免疫耐受,延长移植物的存活时间。

     

  • 图  1  各组小鼠二次移植心脏的Kaplan-Meier生存曲线

    Figure  1.  Kaplan-Meier survival curve of retransplantation heart of mice in each group

    图  2  各组小鼠二次移植心脏组织病理学改变

    注:A、A’图为对照组;B、B’图为实验A组;C、C’图为实验C组;D、D’图为实验D组;A~D图(×100);A’~D’图(×400)

    Figure  2.  Pathological changes of retransplantation heart tissue of mice in each group(HE)

    图  3  各组小鼠移植心脏组织相关因子mRNA相对表达量的比较

    注:与对照组比较,aP<0.05;与实验A组比较,bP<0.05;与实验B组比较,cP<0.05

    Figure  3.  Comparison of mRNA expression of cytokines in transplant heart tissues of mice in each group

    图  4  各组小鼠血清中相关因子浓度的比较

    注:与对照组比较,aP<0.05;与实验A组比较,bP<0.05;与实验B组比较,cP<0.05

    Figure  4.  Comparison of the serum concentration of cytokines in transplant heart tissues of mice in each group

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出版历程
  • 收稿日期:  2016-07-08
  • 网络出版日期:  2021-03-19
  • 刊出日期:  2016-11-15

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