Luminex技术监测抗HLA抗体对肾移植受者预后的影响

林华; 陈洁晶; 陈怀周; 莫秋菊; 眭维国

doi:10.3969/j.issn.1674-7445.2016.05.011

Luminex技术监测抗HLA抗体对肾移植受者预后的影响

doi: 10.3969/j.issn.1674-7445.2016.05.011

541002 广西桂林，解放军第181医院中心实验室广西代谢性疾病研究重点实验室

基金项目:

军队临床高新重大项目 2010gxjs 037

广西自然科学基金 2011GXNSFB018105

广西植物功能物质研究与利用重点实验室开放课题 FPRU2011-2

详细信息

通讯作者:
眭维国, Email：linhuayanbama@126.com

中图分类号: R617
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出版历程
- 收稿日期: 2016-06-10
- 网络出版日期: 2021-01-19
- 刊出日期: 2016-09-15

Effect of anti-HLA antibody detected by Luminex testing on clinical prognosis of renal transplantation recipients

Central Laboratory of the 181^st Hospital of People's Liberation Army, Guangxi Key Laboratory of Metabolic Disease Research, Guilin 541002, China

More Information

Corresponding author: Sui Weiguo, Email：linhuayanbama@126.com

摘要

摘要: 目的探讨Luminex技术检测受者体内抗人类白细胞抗原(HLA)抗体水平在预测肾移植受者预后的价值。方法选取2013年6月至2015年11月在解放军第181医院拟行肾移植患者1 105例(其中354例成功接受肾移植手术)，收集肾移植术前、术后血清标本共1 923例次。采用Luminex技术检测术前和术后的抗HLA抗体的阳性率和抗体荧光强度，同时并对移植受者进行移植肾功能检测。结果术前抗HLA抗体阳性血清样本占总数的51.0%(546/1 071)，其中抗HLA Ⅰ类抗体阳性占26.0%(279/1 071)，抗HLA Ⅱ类抗体阳性占24.9%(267/1 071)，其中抗HLA抗体Ⅰ、Ⅱ类均为阳性占11.4%(122/1 071)。354例肾移植患者中，术后出现抗HLA抗体者占17%(59/354)，其中单独抗HLA Ⅰ类抗体阳性者25例(术后新发阳性4例)，单独抗HLA Ⅱ类抗体阳性者占15例(术后新发阳性1例)，两者皆阳性者19例(术后新发阳性4例)。随访期间，术后抗HLA Ⅰ类、Ⅱ类及两种抗体均阳性者出现移植肾功能异常者分别为13例、5例和11例，其中术后抗体新发阳性者均出现移植肾功能异常。结论 Luminex技术可动态监测肾移植术后受者抗HLA抗体水平，而抗HLA抗体阳性对预测肾移植受者预后有重要的价值。
- 肾移植 /
- Luminex /
- 抗人类白细胞抗原抗体 /
- 移植物排斥
Abstract: Objective To investigate the value of anti-human leukocyte antigen (HLA) antibody level detected by Luminex testing in predicting clinical prognosis of renal transplantation recipients. Methods A total of 1 105 patients scheduled to undergo renal transplantation (354 successfully undergoing renal transplantation) in the 181^st Hospital of Peoples Liberation Army from June 2013 to November 2015 were selected. The serum samples were collected from 1 923 cases before and after renal transplantation. The positive rate and fluorescent intensity of anti-HLA antibody were detected by Luminex testing before and after renal transplantation. The renal function of recipients was also evaluated after renal transplantation. Results Prior to renal transplantation, 51.0% (546/1 071) of serum samples were positive for anti-HLA antibody, including 26.0% (279/1 071) positive for anti-HLA Ⅰ antibody, 24.9% (267/1 071) positive for anti-HLA Ⅱ antibody and 11.4% (122/1 071) positive for both anti-HLA Ⅰ and anti-HLA Ⅱ antibodies. Among 354 patients undergoing renal transplantation, 59 (17%) were positive for anti-HLA antibody after renal transplantation, including 25 (4 newly positive after surgery) positive for anti-HLA Ⅰ antibody, 15 (1 newly positive after surgery) positive for anti-HLA Ⅱ antibody and 19 (4 newly positive after surgery) positive for both anti-HLA Ⅰ and anti-HLA Ⅱ antibodies. During subsequent follow-up, 13 patients positive for anti-HLA Ⅰ antibody, 5 positive for anti-HLA Ⅱ antibody and 11 positive for both anti-HLA Ⅰ and anti-HLA Ⅱ antibodies developed transplant kidney dysfunction. All patients newly positive for anti-HLA antibody after renal transplantation presented with transplant kidney dysfunction. Conclusions Luminex testing can perform dynamic detection of the positive rate of anti-HLA antibody, which is important in predicting clinical prognosis of recipients after renal transplantation.
- Renal transplantation /
- Luminex /
- Anti-human leukocyte antigen(HLA) antibody /
- Graft rejection

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参考文献(17)

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