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经门静脉注射人脐带血间充质干细胞联合多巴胺受体2拮抗剂促进兔缺血型胆道病变胆管修复

洪春虹 赵辉 陈云浩 郑博文 顾世杰 任杰 易述红

洪春虹, 赵辉, 陈云浩, 等. 经门静脉注射人脐带血间充质干细胞联合多巴胺受体2拮抗剂促进兔缺血型胆道病变胆管修复[J]. 器官移植, 2016, 7(3): 177-181. doi: 10.3969/j.issn.1674-7445.2016.03.003
引用本文: 洪春虹, 赵辉, 陈云浩, 等. 经门静脉注射人脐带血间充质干细胞联合多巴胺受体2拮抗剂促进兔缺血型胆道病变胆管修复[J]. 器官移植, 2016, 7(3): 177-181. doi: 10.3969/j.issn.1674-7445.2016.03.003
Hong Chunhong, Zhao Hui, Chen Yunhao, et al. Intraportal injection of human umbilical cord blood-derived mesenchymal stem cells combined with dopamine-2 receptor antagonist accelerates the repairing of bile duct in rabbit models with ischemia-type biliary lesion[J]. ORGAN TRANSPLANTATION, 2016, 7(3): 177-181. doi: 10.3969/j.issn.1674-7445.2016.03.003
Citation: Hong Chunhong, Zhao Hui, Chen Yunhao, et al. Intraportal injection of human umbilical cord blood-derived mesenchymal stem cells combined with dopamine-2 receptor antagonist accelerates the repairing of bile duct in rabbit models with ischemia-type biliary lesion[J]. ORGAN TRANSPLANTATION, 2016, 7(3): 177-181. doi: 10.3969/j.issn.1674-7445.2016.03.003

经门静脉注射人脐带血间充质干细胞联合多巴胺受体2拮抗剂促进兔缺血型胆道病变胆管修复

doi: 10.3969/j.issn.1674-7445.2016.03.003
基金项目: 

2015广东省自然科学基金研究团队项目 2015A030312013

中国肝炎防治基金会天晴肝病研究基金 TQGB20140180

详细信息
    通讯作者:

    易述红,Email:yishuhong@163.com

  • 中图分类号: R617, R-332, R445.1

Intraportal injection of human umbilical cord blood-derived mesenchymal stem cells combined with dopamine-2 receptor antagonist accelerates the repairing of bile duct in rabbit models with ischemia-type biliary lesion

More Information
  • 摘要:   目的  探讨经门静脉注射间充质干细胞(MSC)联合多巴胺受体2拮抗剂(依替必利)促进兔缺血胆管病变(ITBL)胆管修复的作用。  方法  将24只新西兰兔随机分成4组,每组6只,分别为A组(假手术组)、B组(ITBL模型组)、C组(MSC组)、D组(MSC+依替必利组)。监测术前,术后1、4 d,术后1、2、3、4周肝功能指标的变化。术后4周行胆管造影及超声造影,并取肝组织行病理学检查。  结果  与A组比较,术后B组、C组和D组肝功能各指标均出现不同程度的升高(均为P < 0.05)。与B组比较,C组和D组的肝功能指标下降趋势更显著(均为P < 0.05),其中D组下降最明显。胆道超声结果示A~D组的门静脉管径/胆管管径比值分别为(3.2±0.3):1、(1.2±0.2):1、(2.1±0.1):1、(3.0±0.3):1;B组胆管扩张严重,C组程度较轻,D组基本正常。超声造影示D组胆管壁血供改善最显著。病理学检查结果显示D组胆管损伤程度最轻。  结论  经门静脉注射MSC联合多巴胺受体2拮抗剂可以显著促进兔ITBL胆管的修复。

     

  • 图  1  各组兔术后不同时间肝功能的变化

    Figure  1.  Changes of liver function of rabbits in each group at different time after operation

    图  2  各组兔的胆道普通超声表现

    A、B、C、D图分别为A、B、C、D组兔的胆道超声图片,红色箭头表示血管,白色箭头表示胆管

    Figure  2.  Appearance of conventional ultrasonography of bile duct of rabbits in each group

    图  3  各组兔的胆道超声造影表现

    A、B、C、D图分别为A、B、C、D组兔的胆道造影图片;白色箭头表示胆管壁,可见各组回声增强的程度不同

    Figure  3.  Appearance of contrast-enhanced ultrasonography of bile duct of rabbits in each group

    图  4  术后4周各组兔的肝组织病理学表现

    A、B、C、D图分别为A、B、C、D组兔的肝组织病理学检查图片

    Figure  4.  Histopathological changes of rabbits liver tissue in each group at 4 weeks after operation (HE,×200)

  • [1] Ding, DC, Shyu WC, Lin SZ. Mesenchymal stem cells[J]. Cell Transplant, 2011, 20(1): 5-14. doi: 10.3727/096368910X
    [2] Hocking AM, Gibran NS. Mesenchymal stem cells: paracrine signaling and differentiation during cutaneous wound repair[J].Exp Cell Res, 2010, 316(14): 2213-2219. doi: 10.1016/j.yexcr.2010.05.009
    [3] Hoeppner LH, Wang Y, Sharma A, et al. Dopamine D2 receptor agonists inhibit lung cancer progression by reducing angiogenesis and tumor infiltrating myeloid derived suppressor cells[J]. Mol Oncol, 2015, 9(1): 270-281. doi: 10.1016/j.molonc.2014.08.008
    [4] Moreno-Smith M, Lee SJ, Lu C, et al. Biologic effects of dopamine on tumor vasculature in ovarian carcinoma[J]. Neoplasia, 2013, 15(5): 502-510. doi: 10.1593/neo.121412
    [5] Murphy MB, Moncivais K, Caplan AI. Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine[J]. Exp Mol Med, 2013, 45: e54. doi: 10.1038/emm.2013.94
    [6] Peters MA, Walenkamp AM, Kema IP, et al. Dopamine and serotonin regulate tumor behavior by affecting angiogenesis[J]. Drug Resist Updat, 2014, 17(4/5/6): 96-104. http://www.researchgate.net/publication/265909024_Dopamine_and_serotonin_regulate_tumor_behavior_by_affecting_angiogenesis
    [7] Shome S, Rana T, Ganguly S, et al. Dopamine regulates angiogenesis in normal dermal wound tissues[J]. PLoS One, 2011, 6(9): e25215. doi: 10.1371/journal.pone.0025215
    [8] 王桥, 宋伟峰, 易述红, 等.人脐带间充质干细胞移植预防兔缺血性胆道病变的初步实验研究[J].器官移植, 2012, 3(3): 127-132. http://www.cnki.com.cn/Article/CJFDTOTAL-QGYZ201203004.htm

    Wang Q, Song WF, Yi SH, et al. Effect of human umbilical cord-derived mesenchymal stem cells transplantation on ischemic-type biliary lesions rabbit model[J]. Organ Transplant, 2012, 3(3):127-132. http://www.cnki.com.cn/Article/CJFDTOTAL-QGYZ201203004.htm
    [9] Sheng QS, Chen DZ, Lang R, et al. Establishment of an animal model of ischemic type intrahepatic biliary lesion in rabbits[J]. World J Gastroenterol, 2009, 15(6): 732-736. doi: 10.3748/wjg.15.732
    [10] 任杰, 郑博文, 郑荣琴, 等.普通超声及超声造影检测兔胆道缺血模型胆道异常的可行性研究[J].器官移植, 2013, 4(2): 102-106. http://www.organtranspl.com/browse/detail/qkid/63/id/312.html

    Ren J, Zheng BW, Zheng RQ, et al. Feasibility of accessing the abnormal bile duct in biliary ischemia of rabbit model by using conventional and contrast-enhanced ultrasonography[J]. Organ Transplant, 2013, 4(2):102-106. http://www.organtranspl.com/browse/detail/qkid/63/id/312.html
    [11] Lin Q, Wang L, Bai Y, et al. Effect of the co-culture of human bone marrow mesenchymal stromal cells with human umbilical vein endothelial cells in vitro[J]. J Recept Signal Transduct Res, 2016, 36(3): 221-224. doi: 10.3109/10799893.2015.1075043
    [12] Ferrari G, Cusella-D, Angelis G, et al. Muscle regeneration by bone marrow-derived myogenic progenitors[J]. Science, 1998, 279(5356): 1528-1530. doi: 10.1126/science.279.5356.1528
    [13] Maltman DJ, Hardy SA, Przyborski SA. Role of mesenchymal stem cells in neurogenesis and nervous system repair[J]. Neurochem Int, 2011, 59(3): 347-356. http://drcalapai.net/articles/6062
    [14] Wang S, Yang H, Tang Z, et al. Wound dressing model of human umbilical cord mesenchymal stem cells-alginates complex promotes skin wound healing by paracrine signaling[J]. Stem Cells Int, 2016: 3269267. https://www.researchgate.net/publication/288932668_Wound_Dressing_Model_of_Human_Umbilical_Cord_Mesenchymal_Stem_Cells-Alginates_Complex_Promotes_Skin_Wound_Healing_by_Paracrine_Signaling
    [15] Shome S, Dasgupta PS, Basu S. Dopamine regulates mobilization of mesenchymal stem cells during wound angiogenesis[J]. PLoS One, 2012, 7(2): p. e31682. doi: 10.1371/journal.pone.0031682
    [16] Li L, Xia Y, Wang Z, et al. Suppression of the PI3K-Akt pathway is involved in the decreased adhesion and migration of bone marrow-derived mesenchymal stem cells from non-obese diabetic mice[J]. Cell Biol Int, 2011, 35(9): 961-966. doi: 10.1042/CBI20100544
    [17] Ryu CH, Park SA, Kim SM, et al. Migration of human umbilical cord blood mesenchymal stem cells mediated by stromal cell-derived factor-1/CXCR4 axis via Akt, ERK, and p38 signal transduction pathways[J]. Biochem Biophys Res Commun, 2010, 398(1): 105-110. doi: 10.1016/j.bbrc.2010.06.043
    [18] Wu Y, Zhao RC, Tredget EE. Concise review: bone marrow-derived stem/progenitor cells in cutaneous repair and regeneration[J].Stem Cells, 2010, 28(5): 905-915. doi: 10.1002/stem.420/references
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出版历程
  • 收稿日期:  2016-03-09
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2016-05-15

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