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姜黄素预处理对肠缺血-再灌注损伤的作用研究

朱蕾 张丽

朱蕾, 张丽. 姜黄素预处理对肠缺血-再灌注损伤的作用研究[J]. 器官移植, 2016, 7(1): 39-43,60. doi: 10.3969/j.issn.1674-7445.2016.01.007
引用本文: 朱蕾, 张丽. 姜黄素预处理对肠缺血-再灌注损伤的作用研究[J]. 器官移植, 2016, 7(1): 39-43,60. doi: 10.3969/j.issn.1674-7445.2016.01.007
Zhu Lei, Zhang Li. Study on effects of curcumin pretreatment on intestinal ischemia-reperfusion injury[J]. ORGAN TRANSPLANTATION, 2016, 7(1): 39-43,60. doi: 10.3969/j.issn.1674-7445.2016.01.007
Citation: Zhu Lei, Zhang Li. Study on effects of curcumin pretreatment on intestinal ischemia-reperfusion injury[J]. ORGAN TRANSPLANTATION, 2016, 7(1): 39-43,60. doi: 10.3969/j.issn.1674-7445.2016.01.007

姜黄素预处理对肠缺血-再灌注损伤的作用研究

doi: 10.3969/j.issn.1674-7445.2016.01.007
基金项目: 

宜昌市科技基金 A11301-38

详细信息
    通讯作者:

    朱蕾,Email:2473582531@qq.com

  • 中图分类号: R617;R-332

Study on effects of curcumin pretreatment on intestinal ischemia-reperfusion injury

More Information
  • 摘要:   目的  探讨姜黄素预处理对大鼠肠缺血-再灌注损伤(IRI)的作用及其机制。   方法  将30只SD大鼠随机均分为假手术组(Sham组)、IRI组和姜黄素预处理组(Cur组),每组10只。Cur组术前1 h给予姜黄素腹腔注射(5 mg/kg),Sham组和IRI组则给予等体积生理盐水腹腔注射,IRI组和Cur组行小肠IRI手术。观察3组大鼠肠组织病理学变化。采用逆转录聚合酶链反应法检测肠组织白细胞介素(IL)-6、IL-8和肿瘤坏死因子(TNF)-α的信使RNA (mRNA)水平。采用酶链免疫吸附试验(ELISA)检测血清中IL-6、IL-8和TNF-α的蛋白表达水平。采用硫代巴比妥酸反应物法检测肠组织丙二醛(MDA)含量,采用ELISA检测肠组织过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)含量。采用蛋白印迹法检测肠组织中磷脂酰肌醇-3-羟激酶(PI3K)、蛋白激酶(AKT)和哺乳动物雷帕霉素靶蛋白(mTOR)的蛋白表达水平。   结果  与Sham组相比,IRI组肠组织损伤程度,肠组织与血清中的IL-6、IL-8、TNF-α、MDA、PI3K、AKT、mTOR的表达水平均明显升高,而肠组织CAT、GPx与SOD含量明显降低。与IRI组相比,Cur组肠组织损伤程度,肠组织与血清中的IL-6、IL-8、TNF-α、MDA、PI3K、AKT、mTOR的表达水平均明显降低,而肠组织CAT、GPx与SOD含量明显升高(均为P<0.05)。   结论  姜黄素对大鼠发生IRI的肠组织有保护作用,该保护作用可能与抑制PI3K/AKT/mTOR信号通路介导的氧化应激和炎症有关。

     

  • 图  1  3组大鼠肠组织病理学的变化

    注:A图为Sham组;B图为IRI组;C图为Cur组

    Figure  1.  Pathologic changes of intestinal tissue of rats in three groups (HE, ×100)

    图  2  3组大鼠肠组织中PI3K、AKT和mTOR的蛋白表达

    Figure  2.  Protein expression of PI3K、AKT and mTOR in intestinal tissue of rats in three groups

    表  1  本实验所用基因的引物序列

    Table  1.   Primer sequences of genes in the experiment

    基 因 种属 上游引物序列(5’to 3’) 下游引物序列(3’to 5’)
    TNF-α 大鼠 CTGAACTTCGGGGTGATCGG GGCTTGTCACTCGAATTTGAGA
    IL-8 大鼠 CTGCAAGAGACTTCCATCCAG AGTGGTATAGACAGGTCTGTTG
    IL-6 大鼠 AGCTTCCTTGTGCAAGTGTC GACAGCCCAGGTCAAAGGTT
    β-actin 大鼠 AGAGGGAAATCGTGCGTGAC CAATAGTGATGACCTGGCCGT
    下载: 导出CSV

    表  2  3组大鼠肠组织IL-6、IL-8和TNF-α的mRNA表达水平的比较

    Table  2.   Comparison of mRNA levels of IL-6, IL-8 and TNF-α in the intestinal tissue of rats in three groups (x±s)

    组 别 n IL-6 mRNA IL-8 mRNA TNF-α mRNA
    Sham组 10 1.0±0.1 1.0±0.2 1.0±0.1
    IRI组 10 6.0±0.8b 5.0±0.4a 14.5±2.8a
    Cur组 10 2.0±0.3c 2.5±0.3c 6.5±0.8c
    注:与Sham组比较,aP<0.05,bP<0.001;与IRI组比较,cP<0.05
    下载: 导出CSV

    表  3  3组大鼠血清中IL-6、IL-8和TNF-α蛋白表达水平的比较

    Table  3.   Comparison of protein expression levels of IL-6, IL-8 and TNF-α in serum of rats in three groups (x±s, ng/L)

    组 别 n IL-6 IL-8 TNF-α
    Sham组 10 100±20 100±10 100±20
    IRI组 10 900±100b 650±50a 1 700±150a
    Cur组 10 400±50c 350±40c 850±100c
    注:与Sham组比较,aP<0.05,bP<0.001;与IRI组比较,cP<0.05
    下载: 导出CSV

    表  4  3组大鼠肠组织中MDA、CAT、GPx和SOD含量的比较

    Table  4.   Comparison of MDA、CAT、GPx and SOD content in intestinal tissue of rats in three groups (x±s, ng/L)

    组 别 n MDA CAT GPx SOD
    Sham组 10 100±20 100±7 105±15 106±9
    IRI组 10 1 700±300b 35±5a 32±4a 28±5a
    Cur组 10 500±150c 51±5c 60±5c 75±7c
    注:与Sham组比较,aP<0.05,bP<0.001;与IRI组比较,cP<0.05
    下载: 导出CSV

    表  5  3组大鼠肠组织中PI3K、AKT和mTOR蛋白表达水平的比较

    Table  5.   Comparison of protein expression levels of PI3K, AKT and mTOR in intestinal tissue of rats in three groups (x±s)

    组 别 n PI3K AKT mTOR
    Sham组 10 0.07±0.03 0.06±0.02 0.13±0.02
    IRI组 10 0.31±0.03b 0.65±0.07a 0.55±0.05a
    Cur组 10 0.18±0.02d 0.29±0.04d 0.22±0.03c
    注:与Sham组比较,aP<0.05,bP<0.01;与IRI组比较,cP<0.05,dP<0.01
    下载: 导出CSV
  • [1] Gonzalez LM, Moeser AJ, Blikslager AT. Animal models of ischemia-reperfusion-induced intestinal injury: progress and promise for translational research[J]. Am J Physiol Gastrointest Liver Physiol,2015,308(2):G63-G75. doi: 10.1152/ajpgi.00112.2013
    [2] Oltean M. Intestinal preservation for transplantation: current status and alternatives for the future[J]. Curr Opin Organ Transplant,2015,20(3):308-313. doi: 10.1097/MOT.0000000000000187
    [3] Oltean M, Churchill TA. Organ-specific solutions and strategies for the intestinal preservation[J]. Int Rev Immunol,2014,33(3):234-244. doi: 10.3109/08830185.2013.853764
    [4] Lenaerts K, Ceulemans LJ, Hundscheid IH, et al. New insights in intestinal ischemia-reperfusion injury: implications for intestinal transplantation[J]. Curr Opin Organ Transplant,2013,18(3):298-303. doi: 10.1097/MOT.0b013e32835ef1eb
    [5] Wong DM, Moore RM, Brockus CW. Intestinal ischemia-reperfusion injury in horses: pathogenesis and therapeutics[J]. Compend Contin Educ Vet,2012,34(8):E5. http://cn.bing.com/academic/profile?id=2399267034&encoded=0&v=paper_preview&mkt=zh-cn
    [6] Bhattacharyya A, Chattopadhyay R, Mitra S, et al. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases[J]. Physiol Rev,2014,94(2):329-354. doi: 10.1152/physrev.00040.2012
    [7] Von Websky MW, Kalff JC, Schäfer N. Current knowledge on regulation and impairment of motility after intestinal transplantation[J]. Curr Opin Organ Transplant,2015,20(3):303-307. doi: 10.1097/MOT.0000000000000190
    [8] Gibellini L, Bianchini E, De Biasi S, et al. Natural compounds modulating mitochondrial functions[J]. Evid Based Complement Alternat Med,2015:527209. http://cn.bing.com/academic/profile?id=1564135916&encoded=0&v=paper_preview&mkt=zh-cn
    [9] Fiorentini D, Zambonin L, Vieceli Dalla Sega F, et al. Polyphenols as modulators of aquaporin family in health and disease[J]. Oxid Med Cell Longev,2015:196914. http://cn.bing.com/academic/profile?id=1559856110&encoded=0&v=paper_preview&mkt=zh-cn
    [10] Mahmood K, Zia KM, Zuber M, et al. Recent developments in curcumin and curcumin based polymeric materials for biomedical applications: a review[J]. Int J Biol Macromol,2015,81:877-890. doi: 10.1016/j.ijbiomac.2015.09.026
    [11] Ghosh S, Banerjee S, Sil PC. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: a recent update[J]. Food Chem Toxicol,2015,83:111-124. doi: 10.1016/j.fct.2015.05.022
    [12] Shanmugam MK, Rane G, Kanchi MM, et al. The multifaceted role of curcumin in cancer prevention and treatment[J]. Molecules,2015,20(2):2728-2769. doi: 10.3390/molecules20022728
    [13] Fan Z, Yao J, Li Y, et al. Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb[J]. Int J Clin Exp Pathol,2015,8(4):3451-3459. http://cn.bing.com/academic/profile?id=2418151191&encoded=0&v=paper_preview&mkt=zh-cn
    [14] Arumugam TV, Okun E, Tang SC, et al. Toll-like receptors in ischemia-reperfusion injury[J]. Shock,2009,32(1):4-16. doi: 10.1097/SHK.0b013e318193e333
    [15] Aivatidi C, Vourliotakis G, Georgopoulos S, et al. Oxidative stress during abdominal aortic aneurysm repair: biomarkers and antioxidant's protective effect: a review[J]. Eur Rev Med Pharmacol Sci, 2011,15(3):245-252. http://cn.bing.com/academic/profile?id=2098113086&encoded=0&v=paper_preview&mkt=zh-cn
    [16] 马帅军,张更,曹志强,等.肾缺血-再灌注损伤大鼠SDF-1、ICAM-1表达与肾小管坏死评分的相关性研究[J].器官移植,2014,5(5):294-298. http://www.organtranspl.com/browse/detail/qkid/82/id/192.html

    Ma SJ, Zhang G, Cao ZQ, et al. Relationship between the expression of SDF-1,ICAM-1 and renal tubular necrosis score in rats with renal ischemic reperfusion injury[J]. Organ Transplant, 2014,5(5):294-298. http://www.organtranspl.com/browse/detail/qkid/82/id/192.html
    [17] Huang CY, Hsiao JK, Lu YZ, et al. Anti-apoptotic PI3K/Akt signaling by sodium/glucose transporter 1 reduces epithelial barrier damage and bacterial translocation in intestinal ischemia[J]. Lab Invest,2011,91(2):294-309. doi: 10.1038/labinvest.2010.177
    [18] Hao F, Kang J, Cao Y, et al. Curcumin attenuates palmitate-induced apoptosis in MIN6 pancreatic β-cells through PI3K/Akt/FoxO1 andmitochondrial survival pathways[J]. Apoptosis,2015,20(11):1420-1432. doi: 10.1007/s10495-015-1150-0
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出版历程
  • 收稿日期:  2015-10-18
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2016-01-15

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