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心脏死亡器官捐献供者与尸体供者小肠移植物保存质量的比较研究

邱啸臣 李元新 梁玉梅 余琦

邱啸臣, 李元新, 梁玉梅, 等. 心脏死亡器官捐献供者与尸体供者小肠移植物保存质量的比较研究[J]. 器官移植, 2016, 7(1): 26-31. doi: 10.3969/j.issn.1674-7445.2016.01.005
引用本文: 邱啸臣, 李元新, 梁玉梅, 等. 心脏死亡器官捐献供者与尸体供者小肠移植物保存质量的比较研究[J]. 器官移植, 2016, 7(1): 26-31. doi: 10.3969/j.issn.1674-7445.2016.01.005
Qiu Xiaochen, Li Yuanxin, Liang Yumei, et al. Comparative study on the preservation quality of intestinal grafts from donors of donation after cardiac death and cadaveric donors[J]. ORGAN TRANSPLANTATION, 2016, 7(1): 26-31. doi: 10.3969/j.issn.1674-7445.2016.01.005
Citation: Qiu Xiaochen, Li Yuanxin, Liang Yumei, et al. Comparative study on the preservation quality of intestinal grafts from donors of donation after cardiac death and cadaveric donors[J]. ORGAN TRANSPLANTATION, 2016, 7(1): 26-31. doi: 10.3969/j.issn.1674-7445.2016.01.005

心脏死亡器官捐献供者与尸体供者小肠移植物保存质量的比较研究

doi: 10.3969/j.issn.1674-7445.2016.01.005
基金项目: 

首都卫生发展科研专项项目 首发2011-5007-05

详细信息
    通讯作者:

    李元新, Email:liyuanxin1966@163.com

  • 中图分类号: R617;R656.7

Comparative study on the preservation quality of intestinal grafts from donors of donation after cardiac death and cadaveric donors

More Information
  • 摘要:   目的  比较心脏死亡器官捐献(DCD)供者和传统尸体供者小肠移植物的保存质量。  方法  对2013年至2014年在北京地区获取的7例尸体供者(N组)和7例DCD供者(DCD组)的小肠移植物进行质量评估。移植物经灌注、切取, 于保存30 min和6 h采集肠组织, 行组织病理学检查及小肠移植物损伤评分(Chiu氏积分法), 采用硫代巴比妥酸法检测肠组织丙二醛(MDA)含量, 采用dUTP缺口末端标记(TUNEL)法检测肠黏膜细胞凋亡情况。  结果  保存30 min, N组和DCD组的小肠移植物损伤评分分别为(1.46±0.81)分和(1.76±0.21)分; 保存6 h, 两组相应为(3.86±0.42)分和(4.17±0.71)分(均为P > 0.05)。与保存30 min相比, 保存6 h的N组和DCD组的小肠损伤评分明显增加(均为P < 0.05)。保存30 min, N组和DCD组的小肠移植物肠组织中MDA含量分别为(100±10) pmol/mg、(110±13) pmol/mg (P > 0.05);保存6 h, N组和DCD组的MDA含量分别为(170±18) pmol/mg和(310±29) pmol/mg, 同一保存时间两组比较差异有统计学意义(P < 0.05)。与保存30 min相比, 两组小肠移植物切取保存6 h肠组织中的MDA含量明显增加(均为P < 0.05)。保存30 min, N组和DCD组的小肠移植物肠黏膜细胞凋亡数量分别为(9.78±2.56)个和(15.78±2.84)个(P > 0.05);保存6 h, N组和DCD组相应为(31.32±1.38)个和(53.42±1.95)个, 两组比较差异有统计学意义(P < 0.05)。两组小肠移植物肠黏膜细胞凋亡数量保存6 h后较保存30 min时明显增加(均为P < 0.05)。  结论  DCD供者与传统尸体供者的小肠移植物的保存质量相当, 提示DCD供者小肠移植物有可能应用于临床小肠移植。

     

  • 图  1  两组小肠移植物保存不同时间的病理学变化(HE,×40)

    注:A图为N组小肠移植物保存30 min;B图为DCD组小肠移植物保存30 min;C图为N组小肠移植物保存6 h;D图为DCD组小肠移植物保存6 h

    Figure  1.  Pathologic change of intestine grafts of two groups at different preservation time

    图  2  两组小肠移植物不同保存时间的细胞凋亡情况(TUNEL,×400)

    注:A图为N组小肠移植物保存30 min;B图为DCD组小肠移植物保存30 min;C图为N组小肠移植物保存6 h;D图为DCD组小肠移植物保存6 h

    Figure  2.  Cell apoptosis of intestine grafts of two groups at different preservation time

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出版历程
  • 收稿日期:  2015-11-18
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2016-01-15

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