磁性纳米复合物对人肝癌细胞增殖能力的影响

王子真; 胡刚; 吴新淮

doi:10.3969/j.issn.1674-7445.2015.06.016

磁性纳米复合物对人肝癌细胞增殖能力的影响

doi: 10.3969/j.issn.1674-7445.2015.06.016

100700 北京军区总医院放射科

基金项目:

国家自然科学基金 81302550

详细信息

通讯作者:
吴新淮, Email:wuxinzhun@126.com

中图分类号: R617;R657.3;R812
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出版历程
- 收稿日期: 2015-08-01
- 网络出版日期: 2021-01-19
- 刊出日期: 2015-11-15

Effect of magnetic nanocomposites on proliferation ability of human hepatoma carcinoma cells

Department of Radiology, Beijing Military General Hospital, Beijing 100700, China

More Information

Corresponding author: Wu Xinhuai, Email: wuxinzhun@126.com

摘要

摘要: 目的探讨磁性纳米复合物对人肝细胞癌(肝癌)细胞(HepG2细胞株)增殖能力的影响。方法以聚乙烯亚胺包被的磁性纳米复合物(PEI-SPIO)作为基因载体复合富含亮氨酸重复单位的G蛋白耦联受体(LGR)5-小干扰RNA (siRNA), 待细胞融合达60%时转染HepG2细胞建立PEI组, 等量的单纯LGR5-siRNA转染HepG2建立SI组, 另设未转染对照(Ctrl)组。采用MRI T2扫描检测纳米复合物进入细胞的效率, 细胞计数试剂盒(CCK)-8实验检测细胞增殖抑制率, 逆转录聚合酶链反应(RT-PCR)检测细胞LGR5的信使核糖核酸(mRNA)表达水平, 蛋白印迹法检测LGR5、cyclin D1蛋白表达。结果 PEI组HepG2细胞的MRI T2信号明显减低。与Ctrl组相比, PEI组HepG2细胞的细胞增殖抑制率明显升高, LGR5 mRNA的相对表达量和LGR5、cyclin D1蛋白相对表达量均明显降低(均为P < 0.05), 而SI组细胞的相应指标差异无统计学意义(均为P>0.05)。结论磁性纳米复合物PEI-SPIO复合LGR5-siRNA可有效转染HepG2细胞, 其机制可能是通过下调cyclin D1表达水平抑制人肝癌HepG2细胞的增殖能力。
- 纳米复合物 /
- 小干扰核糖核酸 /
- 肝癌细胞 /
- 细胞增殖 /
- 磁共振成像
Abstract: Objective To investigate the effect of magnetic nanocomposites on proliferation ability of human hepatoma carcinoma (HCC) cells(HepG2 cell line). Methods Leucine-rich repeat-containing G protein-coupled receptor (LGR) 5-small interfering ribonucleic acid (siRNA) was composited with polyethylenimine wrapped superparamagnetic iron oxide nanoparticle (PEI-SPIO) as the gene vector. PEI group was established by transfecting HepG2 cells when cell fusion reached 60% and SI group was established by transfecting HepG2 cells with equivalent simple LGR5-siRNA. Control(Ctrl) group was also established without transfecting. The efficiency of nanocomposites entering cells was scanned with MRI T2. The inhibition rate of cell proliferation was detected by (cell count kit, CCK)-8 assay. The expression level of messenger ribonucleic acid (mRNA) in LGR5 of cells was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and the protein expressions of LGR5 and cyclin D1 were detected by western blotting. Results MRI T2 signal of HepG2 cells in PEI group decreased significantly. Compared with Ctrl group, the inhibition rate of cell proliferation of HepG2 cells in PEI group was significantly increased. The relative expression of LGR5 mRNA and the relative expression of LGR5 and cyclin D1 protein were both significantly decreased (all in P < 0.05), while the corresponding indexes of the cells in SI group had no statistical significance (all in P>0.05). Conclusions Magnetic nanocomposites PEI-SPIO composited with LGR5-siRNA may effectively transfect HepG2 cells. Its mechanism may take effect through down-regulating the expression of cyclin D1 to inhibit the proliferation ability of hepatocellular carcinoma HepG2 cells.
- Nanocomposites /
- Small interfering ribonucleic acid /
- Hepatoma carcinoma cell /
- Cell proliferation /
- Magnetic resonance imaging

HTML全文

图 1 各组HepG2细胞MRI T2成像的比较

Figure 1. Comparison of MRI T2 imaging of HepG2 cells in each group

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图 2 各组HepG2细胞功能蛋白表达量的比较

Figure 2. Comparison of functional protein expression of HepG2 cells in each group

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