Repairment of sciatic nerve defect by Schwann cell of adult rat combined with acellular nerve grafts
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摘要:
目的 探讨成年许旺细胞(SC)复合去细胞神经支架构建的组织工程化人工神经移植治疗外周神经损伤的效果。 方法 从Wallerian变性1周的成年SD大鼠远侧端神经中分离培养得到SC, 复合去细胞神经支架构建组织工程化人工神经。移植分为SC+去细胞SD大鼠神经支架组(SC治疗组)和空细胞SD大鼠神经支架组(阴性对照组), 每组各5只。比较两组大鼠术后2、4、8周损伤侧坐骨神经功能指数(SFI), 术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率等修复效果的指标。 结果 SC治疗组术后2、4、8周损伤侧SFI, 术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率均优于或高于阴性对照组(均为P < 0.05)。 结论 采用成年SC复合去细胞神经支架的人工神经移植治疗外周神经缺损, 可有效促进损伤神经的功能恢复。 Abstract:Objective To investigate clinical efficacy of tissue-engineered artificial nerve grafts constructed by acellular nerve grafts combined with adult rat Schwann cell (SC) in the treatment of peripheral nerve injury. Methods SCs were isolated and cultured from the distal nerves of adult Sprague Dawley (SD) rats with 1-week Wallerian degeneration and then combined with acellular nerve grafts to construct tissue-engineered artificial nerve. All rats were divided into acellular nerve graft containing SCs (SC group) and nerve graft containing no cells groups (control group), five animals in each group. At 2-, 4-and 8-week after surgery, sciatic function index (SFI) of the affected side was compared between two groups. At postoperative 8 weeks, nerve conduction of sciatic nerve of the injured side, recovery rate of triceps surae wet weight and other relevant parameters were equally compared between two groups. Results In the SC group, SFI of the affected side at 2-, 4-and 8-week after surgery, nerve conduction of sciatic nerve at the injured side and recovery rate of triceps surae wet weight at postoperative 8 weeks were significantly better compared with those in the control group (all in P < 0.05). Conclusions Combined use of adult rat SCs and acellular nerve grafts effectively repairs peripheral nerve defects and accelerates functional recovery of injured nerves. -
Key words:
- Schwann cell /
- Transplantation /
- Acellular nerve grafts /
- Nerve regeneration
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图 1 相差显微镜下成年大鼠原代SC的形态(×50)
注：A图为相差显微镜下原代SC，细胞较小，呈2个长突起的梭形，也有细胞呈3个突起的三角形，胞体周围有亮晕，另一种成纤维细胞较宽大，呈扁平不规则形；B图为SC的S-100免疫细胞化学染色，SC呈棕色，胞体小，有细长的2个突起，少数的阳性细胞有3个突起，胞体轮廓清晰呈梭形或三角形，扁平不规则的成纤维细胞呈S-100阴性免疫反应
Figure 1. Morphology of adult primary SC under phase-contrast microscope
图 2 两组大鼠术后坐骨神经功能指数的比较
Figure 2. Comparison of the SFI of rats between two groups after surgery
图 3 两组大鼠术后8周肌复合动作电位的比较
Figure 3. Comparison of the CMAP of rats between two groups at 8 weeks after surgery
图 4 两组大鼠术后8周神经传导速度的比较
Figure 4. Comparison of the NCV of rats between two groups at 8 weeks after surgery
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