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成年大鼠骨髓间充质干细胞复合组织工程人工神经修复1 cm坐骨神经缺损

周丽娜 崔晓军 苏开鑫 王晓红 蔡雪彦 郭金华 纳青青

周丽娜, 崔晓军, 苏开鑫, 等. 成年大鼠骨髓间充质干细胞复合组织工程人工神经修复1 cm坐骨神经缺损[J]. 器官移植, 2015, 6(3): 157-161, 168. doi: 10.3969/j.issn.1674-7445.2015.03.006
引用本文: 周丽娜, 崔晓军, 苏开鑫, 等. 成年大鼠骨髓间充质干细胞复合组织工程人工神经修复1 cm坐骨神经缺损[J]. 器官移植, 2015, 6(3): 157-161, 168. doi: 10.3969/j.issn.1674-7445.2015.03.006
Zhou Lina, Cui Xiaojun, Su Kaixin, et al. Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve[J]. ORGAN TRANSPLANTATION, 2015, 6(3): 157-161, 168. doi: 10.3969/j.issn.1674-7445.2015.03.006
Citation: Zhou Lina, Cui Xiaojun, Su Kaixin, et al. Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve[J]. ORGAN TRANSPLANTATION, 2015, 6(3): 157-161, 168. doi: 10.3969/j.issn.1674-7445.2015.03.006

成年大鼠骨髓间充质干细胞复合组织工程人工神经修复1 cm坐骨神经缺损

doi: 10.3969/j.issn.1674-7445.2015.03.006
基金项目: 

国家自然科学基金 81401022

湛江市科技公关计划项目 2012C3102012

广东医学院科研基金项目青年基金 Q2009006

详细信息
    通讯作者:

    周丽娜, Email:21456783@qq.com

  • 中图分类号: R617

Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve

More Information
  • 摘要:   目的  探讨骨髓间充质干细胞(BMSC)作为组织工程化人工神经的种子细胞移植治疗外周神经损伤的效果。  方法  从成年大鼠的骨髓中分离培养得到BMSC, 复合去细胞神经支架构建"组织工程化人工神经"。移植后分为BMSC+去细胞SD大鼠神经导管组(BMSC治疗组)和空细胞SD大鼠神经导管组(阴性对照组), 每组各5只。比较两组大鼠术后2、4、8周损伤侧坐骨神经功能指数(SFI), 术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率等修复效果的指标。  结果  BMSC治疗组的坐骨神经修复术后2、4、8周损伤侧坐骨神经功能指数, 术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率均优于阴性对照组(均为P < 0.05)。  结论  BMSC复合去细胞神经支架的组织工程化人工神经可有效促进神经再生和功能恢复。

     

  • 图  1  相差显微镜下成年大鼠BMSC的形态(×50)

    注:A图为原代BMSC;B图为第10代BMSC

    Figure  1.  Morphology of BMSC of adult rats under phase contrast microscrope

    图  2  两组大鼠术后8周肌复合动作电位的比较

    Figure  2.  Comparison of the CMAP of rats between two groups at 8 weeks after surger

    图  3  两组大鼠术后8周神经传导速度的比较

    Figure  3.  Comparison of the NCV of rats between two groups at 8 weeks after surgery

    图  4  两组大鼠术后8周小腿三头肌湿重恢复率的比较

    Figure  4.  Comparison of the recovery rate of gastrocnemius wet weight of rats between two groups at 8 weeks after surgery

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出版历程
  • 收稿日期:  2015-01-25
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2015-05-15

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