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西罗莫司促进小鼠心脏移植模型中调节性T细胞的分化和增殖

谢江平 张夕凉 刘刚 吴仕和 王育红

谢江平, 张夕凉, 刘刚, 等. 西罗莫司促进小鼠心脏移植模型中调节性T细胞的分化和增殖[J]. 器官移植, 2015, 6(1): 26-30. doi: 10.3969/j.issn.1674-7445.2015.01.006
引用本文: 谢江平, 张夕凉, 刘刚, 等. 西罗莫司促进小鼠心脏移植模型中调节性T细胞的分化和增殖[J]. 器官移植, 2015, 6(1): 26-30. doi: 10.3969/j.issn.1674-7445.2015.01.006
Xie Jiangping, Zhang Xiliang, Liu Gang, et al. Sirolimus promotes differentiation and proliferation of regulatory T cells in mouse heart transplantation model[J]. ORGAN TRANSPLANTATION, 2015, 6(1): 26-30. doi: 10.3969/j.issn.1674-7445.2015.01.006
Citation: Xie Jiangping, Zhang Xiliang, Liu Gang, et al. Sirolimus promotes differentiation and proliferation of regulatory T cells in mouse heart transplantation model[J]. ORGAN TRANSPLANTATION, 2015, 6(1): 26-30. doi: 10.3969/j.issn.1674-7445.2015.01.006

西罗莫司促进小鼠心脏移植模型中调节性T细胞的分化和增殖

doi: 10.3969/j.issn.1674-7445.2015.01.006
详细信息
    通讯作者:

    吴仕和, Email:luckyy666@126.com

  • 中图分类号: R617

Sirolimus promotes differentiation and proliferation of regulatory T cells in mouse heart transplantation model

More Information
  • 摘要:   目的  探讨西罗莫司(SRL)对小鼠异位心脏移植模型的移植物存活时间以及脾脏中调节性T细胞(Treg)分化和增殖的影响。  方法  应用Cuff法建立雄性BALB/c→C57BL/6小鼠颈部异位心脏移植模型。术后随机分为3组各10只受体:对照组术后不予特殊药物治疗, SRL组术后1~14 d予SRL 10 mg/(kg·d)灌胃, 环孢素(CsA)组术后1~14 d予CsA 30 mg/(kg·d)灌胃。记录移植心脏存活时间, 于移植心停搏或术后14 d取脾, 分离单个核细胞, 上流式细胞仪检测CD4+CD25+Treg占CD4+T细胞比例(CD4+CD25+Treg%), 采用逆转录-聚合酶链反应(RT-PCR)法半定量检测Foxp3信使核糖核酸(mRNA)表达水平。  结果  与对照组比较, CsA组和SRL组均明显延长小鼠移植心的生存时间(均为P < 0.01), 而两者之间比较差异无统计学意义(P>0.05)。与对照组比较, CsA组脾脏中CD4+CD25+Treg%明显降低, 而SRL组则明显升高(均为P < 0.01), 后两组比较差异有统计学意义(P < 0.01)。SRL组脾脏T细胞的Foxp3 mRNA表达明显高于对照组和CsA组, 其中对照组亦明显高于CsA组(均为P < 0.01)。  结论  在小鼠心脏移植模型中, SRL明显延长移植物的存活期, 促进CD4+CD25+Treg的增殖与生长, 有利于免疫耐受的形成。

     

  • 图  1  各组小鼠心脏移植术后移植心Kaplan-Meier生存曲线

    Figure  1.  Kaplan-Meier curve of survival time of mice graft heart in each group after heart transplantation

    图  2  各组小鼠心脏移植术后14 d的脾脏CD4+CD25+Treg变化

    Figure  2.  Changes of CD4+CD25+Treg in spleen of mice in each group at 14 d after heart transplantation

    图  3  各组小鼠心脏移植术后14 d的脾脏Foxp3 mRNA表达的变化

    Figure  3.  Changes of expression of Foxp3 mRNA in spleen of mice in each group at 14 d after heart transplantation

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出版历程
  • 收稿日期:  2014-11-22
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2015-01-15

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