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大鼠肾移植模型手术改良技巧探讨

李偲 朱毅 叶少军 周威 刘洪 牛英 佘兴国 明英姿

李偲, 朱毅, 叶少军, 等. 大鼠肾移植模型手术改良技巧探讨[J]. 器官移植, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007
引用本文: 李偲, 朱毅, 叶少军, 等. 大鼠肾移植模型手术改良技巧探讨[J]. 器官移植, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007
Li Cai, Zhu Yi, Ye Shaojun, et al. Modified technique of renal transplantation model in rats[J]. ORGAN TRANSPLANTATION, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007
Citation: Li Cai, Zhu Yi, Ye Shaojun, et al. Modified technique of renal transplantation model in rats[J]. ORGAN TRANSPLANTATION, 2014, 5(3): 156-160. doi: 10.3969/j.issn.1674-7445.2014.03.007

大鼠肾移植模型手术改良技巧探讨

doi: 10.3969/j.issn.1674-7445.2014.03.007
基金项目: 

湖南省自然科学基金 12JJ3115

详细信息
    通讯作者:

    明英姿, Email:myz_china@aliyun.com

  • 中图分类号: R617

Modified technique of renal transplantation model in rats

  • 摘要:   目的  探讨大鼠肾移植模型手术的改良方法。  方法  供体Sprague-Dawley(SD)大鼠21只, 受体Wistar大鼠42只。采用双侧供肾。受体左肾切除后借助自制导管, 行受体肾动脉与供体肾动脉、受体肾静脉与供体下腔静脉端端吻合, 供体输尿管带膀胱瓣与受体膀胱吻合, 最后切除右肾, 腹腔内注入头孢米诺10 mg, 关腹。记录手术时间, 动、静脉吻合时间, 冷、热缺血时间等手术数据; 术后大鼠存活3 d认为模型建立成功, 计算建模成功率, 分析死亡原因。  结果  供体手术时间为(32.7±5.6)min, 供肾修整时间为(4.2±1.1)min。受体手术时间为(42.3±4.9)min, 其中动脉吻合时间为(10.1±3.2)min, 静脉吻合时间为(13.9±2.5)min, 尿路重建时间为(6.3±1.4)min。热缺血时间为(5.4±1.8)s, 冷缺血时间为(56.2±7.3)min。42只受体大鼠中, 建模成功40只, 成功率为95%。另2只受体大鼠死亡, 其中1只死于血管吻合口出血, 1只死于尿瘘引致的腹膜炎。  结论  采用改良的血管端端吻合法建立大鼠肾移植模型具有操作简单、手术时间短、成功率高的特点。

     

  • 图  1  修整后的SD大鼠供肾

    Figure  1.  The donor kidney of SD rat after repairment

    图  2  大鼠肾移植模型的供体与受体肾动、静脉吻合手术示意图

    注:A图为供、受体肾动脉内套入自制套管以备吻合;B图为将供、受体肾动脉吻合口两点各固定1针;C图为先连续缝合吻合口后壁,前壁间断缝合但不打结;D图为退出自制套管;E图为拉紧前壁缝合线并打结固定;F图为受体肾静脉与供体后腔静脉近心端吻合;G图为借助自制套管,受体肾静脉与供体后腔静脉先固定两点;H图为吻合后从供体后腔静脉远心端拔出套管;I图为结扎下腔静脉远心端

    Figure  2.  Schematic diagram of anastomosis surgery of renal artery and vein between donors and recipients of renal transplantation model in rats

    图  3  肾移植大鼠模型再灌注后的移植肾外观图

    注:图中可见开放动、静脉并复温后的移植肾,肾脏色泽红润,表面张力好,血供良好,血管吻合成功

    Figure  3.  External view of transplant kidney after reperfusion of renal transplantation model in rats

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出版历程
  • 收稿日期:  2014-02-10
  • 网络出版日期:  2021-01-19
  • 刊出日期:  2014-05-15

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