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生长抑制因子4表达水平与肝癌肝移植术后复发转移及预后的关系

罗来邦 李新长 张友福 徐志丹 张锡泉

罗来邦, 李新长, 张友福, 等. 生长抑制因子4表达水平与肝癌肝移植术后复发转移及预后的关系[J]. 器官移植, 2014, 5(2): 113-118. doi: 10.3969/j.issn.1674-7445.2014.02.012
引用本文: 罗来邦, 李新长, 张友福, 等. 生长抑制因子4表达水平与肝癌肝移植术后复发转移及预后的关系[J]. 器官移植, 2014, 5(2): 113-118. doi: 10.3969/j.issn.1674-7445.2014.02.012
Luo Laibang, Li Xinchang, Zhang Youfu, et al. Relationship between expression of inhibitor of growth 4 and recurrence/metastasis and prognosis of liver transplantation for hepatocellular carcinoma[J]. ORGAN TRANSPLANTATION, 2014, 5(2): 113-118. doi: 10.3969/j.issn.1674-7445.2014.02.012
Citation: Luo Laibang, Li Xinchang, Zhang Youfu, et al. Relationship between expression of inhibitor of growth 4 and recurrence/metastasis and prognosis of liver transplantation for hepatocellular carcinoma[J]. ORGAN TRANSPLANTATION, 2014, 5(2): 113-118. doi: 10.3969/j.issn.1674-7445.2014.02.012

生长抑制因子4表达水平与肝癌肝移植术后复发转移及预后的关系

doi: 10.3969/j.issn.1674-7445.2014.02.012
基金项目: 

基金项目 项目编码

详细信息
    通讯作者:

    李新长,Email:lixinchang863@163.com

  • 中图分类号: R617

Relationship between expression of inhibitor of growth 4 and recurrence/metastasis and prognosis of liver transplantation for hepatocellular carcinoma

  • 摘要:   目的   探讨生长抑制因子4(ING4)表达水平与原发性肝癌(肝癌)肝移植术后复发转移及预后的关系。   方法   选取2003年5月至2007年12月在江西省人民医院器官移植科实施肝癌肝移植手术的30例患者作为研究对象。组织标本取自手术切除的肝癌组织和相应癌旁1 cm外的癌旁肝组织(PCLT)。采用免疫组织化学(免疫组化)方法对肝癌组织和PCLT中ING4蛋白的表达进行定性检测,比较两者ING4蛋白的阳性表达率及表达强度的差异。采用蛋白质印迹法检测上述两组织ING4蛋白的表达水平。根据蛋白质印迹法结果,计算肝癌组织和PCLT的ING4蛋白表达量之比,以比值0.48为界,将30例患者分为ING4低表达组(15例)和ING4高表达组(15例),分析两组患者临床病理特征、肝癌肝移植术后复发转移和预后情况。   结果   免疫组化结果显示肝癌组织的ING4阳性表达率为63%(19/30),PCLT为93%(28/30),两者比较差异有统计学意义(P<0.05)。肝癌组织的ING4强阳性表达率为27%(8/30),PCLT为63%(19/30),两者比较差异有统计学意义(P<0.05)。蛋白质印迹法结果显示肝癌组织的ING4蛋白阳性率为70%(21/30),PCLT为97%(29/30),两者比较差异有统计学意义(P<0.05)。PCLT的ING4蛋白表达水平高于肝癌组织(197±31比109±24,P<0.05)。30对标本中有25对ING4表达水平在肝癌组织中下调,下调率为83%。在临床病理学特征方面,ING4低表达组和ING4高表达组在肿瘤结节数目(P=0.025)、静脉浸润(P=0.035)、肝外转移(P=0.017)和分化程度(P=0.025)的差异具有统计学意义。ING4高表达组的累计术后复发转移率低于ING4低表达组,生存率高于ING4低表达组(均为P<0.05),ING4高表达组中位生存时间长于ING4低表达组(720 d比540 d P<0.05)。   结论   ING4蛋白水平在肝癌组织中表达下调,且与肝癌肝移植术后复发转移有关,影响肝癌肝移植患者预后。

     

  • 图  1  肝癌组织和癌旁肝组织中ING4蛋白的表达(SABC法,×400)

    Figure  1.  Expression of ING4 protein in hepatocellular carcinoma tissue and pericarcinomatous liver tissue

    图  2  ING4蛋白在肝癌组织、癌旁肝组织和正常肝组织中的表达(蛋白印迹法)

    Figure  2.  Expression of ING4 protein in hepatocellular carcinoma tissue,pericarcinomatous liver tissue and normal liver tissue

    图  3  两组肝癌肝移植患者累计术后复发转移率的比较

    Figure  3.  Comparison of accumulative recurrence/metastasis rate between two groups after liver transplantation for hepatocelluar carcinoma

    图  4  两组肝癌肝移植患者术后生存率的比较

    Figure  4.  Comparison of survival rate between two groups after liver transplantation for hepatocelluar carcinoma

    表  1  ING4低表达组和高表达组肝癌患者临床病理学特征的比较

    Table  1.   Comparison of clinical pathological characteristics between ING4 low expression group and high expression group [n(%)]

    组 别n肿瘤结节数目静脉浸润肝外转移分化程度
    单个多个Ⅰ~Ⅱ级Ⅲ~Ⅳ级
    ING4低表达组155(33)10(67)7(47)8(53)6(40)9(60)3(20)12(80)
    ING4高表达组1512(80)3(20)1(7)14(93)0(0)15(100)10(67)5(33)
    P0.0250.0350.0170.025
    下载: 导出CSV
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出版历程
  • 收稿日期:  2013-10-17
  • 网络出版日期:  2021-01-26
  • 刊出日期:  2014-03-15

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